Pablo Cuate Biochemistry Lab Report Objective: The purpose of this lab is to analyze enzyme activity at pH neutral for acid phosphatase as the colorless p-nitrophenylphosphate (PNPP) substrate concentration changes. We will empirically determine the concentration of production formation‚ Vmax and Km from absorbance of yellow PNP in the reaction. Results Figure 1 This figure indicates a positive linear trend for the concentration of PNP and absorbance. Table 1: Absorbance Value for Acid Phosphatase
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Abstract To determine the rate law with respect to bleach and blue dye‚ a series of graphic and algebraic manipulations were done on the data obtained in the kinetic trace experiment in order to determine the different components that made up the rate law. Using absorbance spectroscopy to monitor concentration over time‚ rate order of the dye was found to be 1st order through the integrated rate law and through the proportionality method; the order of the bleach was also determined to be 1st order
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Another postulate of the kinetic molecular theory is that gas particles are always in motion‚ like the other states of matter. But they are different in that they undergo random translational movement. In solids‚ the particles mainly experience vibrational motion and in liquids they mainly vibrate and rotate‚ with some translational motion. Gas particles move rapidly in straight lines‚ unless acted upon by another particle or the walls of a container. This continuous contact with the container leads
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Chemistry 12: Reaction Kinetics Review 1. Looking at the expressions for reaction rate‚ write expressions with which you could express rates for the following reactions. (Hint: look at what happens to reactants and products.) Recall that solid or liquids can lose or gain mass‚ gases can lose or gain volume and aqueous solutions can increase or decrease in concentration. ("a" is done as an example.) a) Mg(s) + 2HCl(aq) H2(g) + MgCl2(aq) reaction rate = mass of Mg consumed unit time b) c) or
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Abstract This experiment is to study and measure the enzyme activity of β-galactosidase in the different concentrations of o-Nitrophenylgalactoside (ONPG) using a spectrophotometer. The spectrophotometer was also set at 420nm‚ a wavelength which is best for recording the absorbance values for the experiment. From the results‚ 0.9mM ONPG solution has the highest absorbance and 0.1mM ONPG solution has the least. Also‚ 0.5mM ONPG solution has the highest rate of enzyme activity and it is the most efficient
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EXPERIMENT 3-CHEMICAL KINETICS: THE IODINE-CLOCK REACTION J.CHAN1 and C.CABANLIG2 1NATIONAL INSTITUTE OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY‚ COLLEGE OF SCIENCE 2NATIONAL INSTITUTE OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY‚ COLLEGE OF SCIENCE UNIVERSITY OF THE PHILIPPINES‚ DILIMAN‚ QUEZON CITY 1101‚ PHILIPPINES DATE SUBMITTED: JANUARY 8‚ 2013 DATE PERFORMED: DECEMBER 5‚ 2012 ------------------------------------------------- ABSTRACT The kinetics of the reaction between persulfate (S2O82-)
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It was in August 1998 that the first chinks in the Kinetic Honda Motors Ltd. (Kinetic Honda) armor were reported by Business India. Both Honda and the Firodias of Kinetic were quick to deny rumors of a split‚ though reports of the Firodias quietly raising resources to buy out Honda’s stake kept surfacing. The Firodias were even reported to have securitised the assets of their two-wheeler finance company - 20th Century Kinetic Finance (TCKF) - to raise this money. Trouble had been brewing since
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A Study of Azeotrope and Acetone/Chloroform Liquid-Vapor Phase Diagram Abstract: Liquid-vapor phase of acetone/chloroform was studied through distilling a series of mixtures with different mole fraction. When the mixtures were boiling‚ their vapor was condensed through a water column and collected in a receiving container. Refractive index was collected for starting mixture‚ distillate and residue for each sample. A boiling temperature versus acetone’s mole fraction was constructed to show the
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Printedin the Netherlands. Kinetics of growth and sugar consumption in yeasts J ohannes R van Dijken‚ Ruud A. Weusthuis & Jack T. Pronk D epartment of Microbiology and Enzymology‚ Kluyver Laboratory of Biotechnology‚ Julianalaan 67‚ 2628 BC Delft‚ The Netherlands K ey words: a lcoholic fermentation‚ chemostat culture‚ Crabtree effect‚ respiration‚ Saccharornyces cerevisiae‚ y easts A bstract A n overview is presented of the steady- and transient state kinetics of growth and formation of
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.Potential energy and kinetic is what makes the roller coaster ascends up the hill and it descends Roller coasters the change of ds down the hill. Potential and kinetic energy is what makes the roller coaster move. What some people may not realize is that the roller coaster has no engine. When you’re going down hill they use different types of wheels to keep the ride smooth. A roller coaster is not pulled by cable. The 3 types of wheels are running wheels which guides the roller coaster‚ Friction
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