Abstract This experiment is to study and measure the enzyme activity of β-galactosidase in the different concentrations of o-Nitrophenylgalactoside (ONPG) using a spectrophotometer. The spectrophotometer was also set at 420nm‚ a wavelength which is best for recording the absorbance values for the experiment. From the results‚ 0.9mM ONPG solution has the highest absorbance and 0.1mM ONPG solution has the least. Also‚ 0.5mM ONPG solution has the highest rate of enzyme activity and it is the most efficient
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In Life in the Iron Mills the narrator’s purpose is to inform the readers of the cruel realities of the lower class in hopes of change in the social structure. Davis captivates the attention of the reader’s by vividly describing Industrial America from the nightmare fog that covers the town to the hellish life of an industrial worker. Additionally‚ she creates sympathy by sharing the story of Hugh Wolfe; an iron mill worker with the desire for more‚ but with no opportunities is left with no hope
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story line. The “ Life in the Iron-Mills” revolves around Hugh and Deborah Wolfe. It is taken place in the mid 1800s in an unknown factory ridden town. The narrator tells the story through first person as middle class citizen of unknown origin or gender. I believe this story mainly is a representation of the corrupt and unequal social structure that the author may have been living in at the time. The Story starts out with a detailed description of the “town of iron-works”‚ that they live in. The
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project and why are they important? There are three principles or characteristics that any project has. These are time‚ cost and quality. These three are called iron triangle. These are important because they are well known success measure criteria of any project. It places cost‚ time and money at the center of project. This is called Iron Triangle. This framework helps to evaluate and balance the competing demands of cost‚ time and quality within their projects. The quality management of a project
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Iron-Jawed Angels: Movie Analysis1 After watching “Iron Jawed Angels” I gained a strong sense of reality when witnessing what women had to do to achieve their independence and gain a place in a male dominated society. Up until the late 19th century‚ women were perceived as homemakers and were allowed only domestic duties in society but with the emerging industrial and political system women could now use their domestic skill to propel their voice in American government and society. “Dress up
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Enzyme Kinetics Examples and Problems 1. An enzyme is produced for producing a sun protection lotion. Given kinetic data for the enzyme reaction with Vm=2.5 mmol/m3.s‚ Km=8.9 mM and So=12mM‚ what would be the time required for 95% conversion in a batch reactor? 2. An enzyme was assayed at an initial substrate concentration of 10-5M. The Km’ for the substrate is 2x10-3M. At the end of 1 min‚ 2% of the substrate had been converted to product. a. What % of the substrate will be converted
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Chemistry 211 The Formula of Lead (II) Iodide The Analysis of a Chemical Reaction Introduction This experiment is based upon a discovery made through the isolation of PbI2 using a particular scientific method‚ which studies ratios of lead to iodine. Precipitation reactions occur when oppositely charged ions attract and form an insoluble ionic solid. This experiment examines the precipitation reaction between lead (II) nitrate‚ Pb(NO3)2‚ and potassium iodide‚ KI. Both are dissolved in water and
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amount of people who have decided to just not vote. It’s hard to believe it was unavailable to the country’s women until 1920‚ and not until after quite a fight and yet we still have so many people who take the responsibility to vote so lightly. Iron Jawed Angels is a movie that I had previously heard about and was on my list of movies I wanted to watch. I was excited that we had the opportunity to view it during this class. Before I had only heard in brief what women did to earn the right to vote
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------------------------------------------------- Magnetism From Wikipedia‚ the free encyclopedia This article is about magnetic materials. For information about objects and devices that produce a magnetic field‚ see magnet. For fields that magnets and currents produce‚ see magnetic field. For other uses of "Magnetism"‚ see Magnetism (disambiguation). Magnetic leads here. For other uses of magnetic‚ see Magnetic (disambiguation) A magnetic quadrupole Electromagnetism | | * Electricity
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types of forces involved. Two of the main forces experienced are called potential energy and kinetic energy. Potential energy is the energy an object has depending on the position of it. For example the higher an object is the higher the potential energy is and the lower an object is the less potential energy it will have. Kinetic energy is also a main type of force that is involved with rollercoasters. Kinetic energy is the energy an object will have as it is in motion. There are many different types
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