NAME: Aleema Chelsea Chinchamee LAB PARTNERS: Karishma Ramrattan‚ Vishma Ramsumair and Sharona Badree ID #: 814003081 DATE (of lab session): Tuesday 24th March‚ 2015 DEMONSTRATOR: Maurissa Course Code & Title: BIOL 1362- Biochemistry I Title of Lab: Investigating Enzymatic Activity in Sweet Potato‚ Irish Potato Extract and Milk. Aims: 1. To determine the effect of ascorbic acid on Polyphenol Oxidase (Phenolase). 2. To determine the level of specificity of Phenolase using the
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SCH4U7-02 Monday‚ October 21‚ 2013 Designing an Experiment to Investigate Factors Affecting Rate of Reaction Introduction Hydrogen Peroxide (H2O2)‚ is the most simple peroxide and commonly used in several household items such as toothpaste or as an alternative to bleach. However hydrogen peroxide it is a very dangerous substance when accumulated in large amounts. If that situation occurs this substance must be decomposed. But how can we decompose this reaction? Well it decomposes by itself
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shape of the enzyme‚ not allowing it to do its function. Testing on peroxidase- an enzyme- will be done on an animal enzyme (chicken liver) and a plant enzyme (potato). For testing Hydrogen Peroxide (H2O2) will be poured over the enzymes. When the enzymes in the potato or liver combine with the substrate Hydrogen Peroxide the enzymes break and form a chemical reaction 2H2O2 à 2H2O + O2. The more bubbling occurring‚ the more of a reaction there is. Hypothesis- 1. The rate of reaction in the potato
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breaking apart‚ is sped up. An enzyme breaks apart the substrate in the active site of the enzyme; this is where the magic happens. Substrate is what is being broken apart by the enzyme. In this case‚ the enzyme is catalase and the substrate is hydrogen peroxide‚ or 2H2O2. As the catalase and the substrate interact‚ the substrate is brought into the catalase and broken apart into water and oxygen. This process may be able to happen naturally‚ but the enzyme‚ catalase‚ speeds up the process.
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the empirical formula CH2 O? 1) CH3OH 2) CH3CH2OH 3) HCOOH 4) CH3COOH 10. Which list consists of types of chemical formulas? 1) atoms‚ ions‚ molecules 2) metals‚ nonmetals‚ metalloids 3) empirical‚ molecular‚ structural 4) synthesis‚ decomposition‚ neutralization 11. What is the total number of neon atoms contained in 20.2 grams of neon gas? 3) 3.01 × 1023 1) 1.01 × 1024 2) 2.02 × 1024 4) 6.02 × 1023 12. Given the balanced equation: X + Cl2 - C2H5Cl + HCl Which molecule is represented
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temperature and concentrations of different components are altered. In this lab experiment‚ we will be doing an in-depth research of exactly what happens to the enzymes‚ when it happens‚ and why it denatures the way it does. b. Hypothesis Materials and Methods a. Materials 50ml beaker of fresh potato catalase Reaction chamber Ring stand and clamp 10ml graduated cylinder 100ml graduated cylinder 3% hydrogen peroxide Pan (water bath) Pipette Hot plate Ice Thermometer Boiled Catalase
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(n.d.). Retrieved October 29‚ 2013‚ from http://prezi.com/wii0fgw48euc/potatoe-enzyme-lab/ What enzyme is present in liver and potatoes. (n.d.). Retrieved October 29‚ 2013‚ from http://wiki.answers.com/Q/What_enzyme_is_present_in_liver_and_potatoes#slide2 What Is the Optimum PH for Catalase - Ask.com. (n.d.). Retrieved October 29‚ 2013
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The purpose of this lab is to determine the rate of reaction under different circumstances. Different variables will be manipulated to discover their effects on a particular reaction. Changes in temperature‚ pH‚ and enzyme concentration are examples of factors that have the potential to affect the initial rate of an enzyme catalyzed reaction in a controlled experiment‚ whether it be speeding the reaction up or slowing it down. Part I of the experiment establishes a baseline that can be used to compare
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optimum operation of enzymes. These condition include temperature‚ enzyme concentration‚ substrate concentration‚ acidity‚ salinity‚ and any present activators/inhibitors. In this particular lab‚ temperature was the environmental factor studied. More specifically‚ the enzyme catalase and its substrate hydrogen peroxide were tested under different temperatures. It was discovered that‚ temperature can affect the optimum operation of enzymes; The enzyme catalase has an optimum operation condition (in this
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Enzyme Lab Experiments Problem: How can we demonstrate how enzymes work? What happens if we alter the environment of an enzyme? Materials: G;lucose Test Strips Test Tubes Pipettes Raw Hamburg Lettuce Potato Raw Liver Chalk Beakers Dairy Lactose Tablet Water Sugar Solo Cups Hot Plate Knife Gloves Skim Milk Glow Sticks Peroxide Hypothesis: 1. If we change the environment via temperature the glow stick will Its intensity will change 2. If hydrogen peroxide is added to a certain food liver
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