Abstract: Enzymes‚ molecules that speed up chemical reactions‚ are specific to one substrate. In this experiment the substrate hydrogen peroxide and the enzyme catalase will be used. The higher the concentration of potato extract‚ or catalase‚ the faster the reaction and the more substrate present will result in a decrease in the time of the reaction. The amount of concentrations of enzymes and substrates are changed to determine if the reaction is further catalyzed by a greater concentration of
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Abstract In this experiment‚ my lab partners and I tested how time effects a catalase reaction. The amount of hydrogen peroxide was recorded after the reaction for the certain time given has taken place. We used sulfuric acid to stop the reaction with the catalase from occurring. This process is known as denaturing (Campbell 152). The potassium permanganate in this experiment was used as hydrogen peroxide indicator. It determined the amount of hydrogen peroxide remaining after the reaction occurred
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1. Are any of the lab values in Table 1 out of normal range? Are they too high or too low? Her serum creatinine is high. Creatinine is completely filtered from the blood (not as well of a marker for kidney function as inulin because some is secreted‚ but still a good marker of kidney function) and excreted in urine so for her to have more than 0.6-1.2mg/dL in her blood is not normal. Her blood urea nitrogen (BUN) levels are also very high. They should only be around 7-18 mg/dL. Her serum calcium
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bait “Glow sticks have been mentioned briefly in the literature as a means to increase capture rates of aquatic amphibians” (Kristine L. Grayson 1996). In this process of chemiluminescence what happens “in Chemiluminescent Systems wherein a hydrogen peroxide component and an oxalate ester-fluorescer component are mixed and reacted to produce light” (Victor B World
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Unknown Lab Report Microbiology Unknown A Sonia Kabra November 26‚ 2014 Introduction There are numerous reasons for identifying unknown bacteria. Some of these organisms have distinct qualities that set them apart from one another‚ such as the exposure to certain environments. Through out the semester in the laboratory‚ we are able to encounter some of the few microorganisms that we as humans have come into contact with. With the knowledge gained from the sessions in the laboratory‚ we can now
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facilitating reactions that are necessary for an organism to live. Enzymes can be very specific to what environment they function best in1. Numerous environmental impacts were tested for the enzyme peroxidase which catalyzes the decomposition of hydrogen peroxide. The basic decomposition reaction was carried out first without any environmental alterations. The hypothesis for this reaction was supported. The enzyme caused the amount of absorbance increase as time went on at a fairly constant rate (Figure 1)
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heated to a temperature above 70ºC‚ the enzyme will be dead when it is boiled. Based on the effects of the hydroxylamine treated extract‚ the original hypothesis is again accepted. Because the inhibitor‚ hydroxylamine‚ blocked the substrate of hydrogen peroxide from entering the active site‚ the absorbance units decreased compared to the normal extract absorbance units. Further work that would be needed to test explanations would include a more variety of temperatures as well as other enzymes and compare
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Toxicology Lab 1. In this investigation‚ a wide range of concentrations of Sodium Chloride (NaCl) solution were created and the effects that they had on radish seeds were tested. This ultimately created a doseresponse experiment in which it was detectable whether or not radish seeds were a reliable bioassay for the toxicity of NaCl. The goal of this experiment was to determine a correlation between toxicity and seed germination/radicle
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factors affecting the kinetics of reaction between peroxodisulfate (vi) and iodide d. del prado1 and j. belano2 1 department of food science and nutrition‚ college of home economics 2 department of food science and nutrition‚ college of home economics university of the philppines‚ diliman‚ quezon city 1101‚ philippines date submitted: january 7‚ 2013 ------------------------------------------------- ------------------------------------------------- ABSTRACT -------------------------------------------------
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LAB REPORT NUMBER TWO DATE: 3/25/2010 inal attachment Lab Experiment number 11 PURPOSE: To learn the Gram stain technique‚ the reason for the stain‚ and how to identify the results of the organisms stained. MATERIALS: Bunsen burner‚ inoculating loop‚ staining tray‚ glass slides‚ bibulous paper‚ lens paper‚ oil‚ and microscope METHODS: Apply Crystal Violet (Primary stain) for 1 minute. Rinse with D-water Apply Iodine (Mordant) for 1 minute. Rinse with D-water. Apply Alcohol (Decolorize) for
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