Activity of the Enzyme Catalase in breaking down Hydrogen Peroxide and the effect of various factors on Enzyme Activity Introduction The enzyme catalase is present in cells in order to speed the breakdown of hydrogen peroxide (H2O2)‚ which is a toxic chemical to the human body. When hydrogen peroxide is broken down‚ the end products are Water (H2O) and Oxygen (O2). In this report‚ the reaction of catalase to hydrogen peroxide is being tested. Furthermore‚ the effects of temperature‚ concentration
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I. Experiment 1 A. Description of Experiment: Three plastic beakers were used for this experiment. In one‚ a 20 mL of 1% hydrogen peroxide solution was made. In order to get this‚ the stock solution‚ which was 3%‚ was diluted using 10 mL of hydrogen peroxide and 20 mL of water. In the second beaker‚ a ¼ dilution of potato extract was made using 2 mL of potato extract and 6 mL of water. The third plastic beaker contained 8 mL of water. Using the first and second beaker the experimental assay
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LibraryPirate LibraryPirate Metric Prefixes Multiple 1‚000‚000‚000‚000‚000‚000 1‚000‚000‚000‚000‚000 1‚000‚000‚000‚000 1‚000‚000‚000 1‚000‚000 1‚000 100 10 1 0.1 0.01 0.001 0.000001 0.000000001 0.000000000001 0.000000000000001 0.000000000000000001 1018 1015 1012 109 106 103 102 101 1 10–1 10–2 10–3 10–6 10–9 10–12 10–15 10–18 Name exa peta tera giga mega kilo hecto deka — deci centi milli micro nano pico femto atto Abbreviation E P T G M k h da — d c m m n p f a Physical Constants Acceleration
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Lab Experiment 8: Oxidation Puzzle Post Lab Report for 2-ethyl-1‚3-hexandiol Calculations Theoretical yield: 1.857g Product Yield: 1.055g ----> Percent yield = (1.055g/1.87g) x 100% = 56.41% Starting amount of diol: 1.184g ----> Percent Yield = (1.184g/1.87g) x 100% = 63.32% Spectroscopy O-H (Stretch‚ H-bonded) C-H (Stretch) C-H (2720-2820 cm-1) Carbonyl C-O (Stretch) Product wavelength cm-1 3422 Strong‚ Broad 2877‚2936‚2964 Strong‚ Medium None Present 1705 Strong
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Grade 12 Biology (SBI 4U1) Nicole Mikulis Unit: Biochemistry Sept 14 2012 Lab: Effect of temperature and pH on catalase activity BACKGROUND Catalase is an enzyme that detoxifies chemicals that might harm the cell such as hydrogen peroxide (H2O2). The enzyme breaks H2O2 into water and oxygen. The production of the oxygen gas bubbles serves as evidence that the catalase enzyme is working. As catalase is breaking the bonds between H2O2‚ it is releasing energy in
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Period 12 Chemical Aspects of Life & Spit Lab ABSTRACT: The objective for the Spit lab was to test two different types of crackers for the presence or absence of starch and of reducing sugars. Also‚ to test the chewed cracker‚ the one that didn’t have a reducing sugar‚ for the presence or absence of a reducing sugar with the saliva in it. Adding on‚ another part of the objective is to determine the effect of amylase on starch. For the Chemical Aspects lab‚ the objectives were to test for the presence
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your mass to 2 decimal places on your lab sheet. 4. Zero the balance. 5. Add 3-4 grams of zinc to the vial and determine the mass of the zinc. Record on your lab sheet. 6. Go to one of the burets set up around the classroom. 7. Look at the buret and record the volume on your lab sheet. 8. Add 10 to 15mL of acid to your vial and record the ending volume on your lab sheet. 9. Make a drawing in the space provided of the vial and its contents on the lab sheet. Be sure to label all the chemicals
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Name: Derell Ruan Form: 4B1 SBA: Chemistry Aim: To determine which gas‚ Ammonia or hydrogen chloride defuses faster. Hypothesis: Ammonia will defuse faster than hydrogen chloride. Materials Equipment: Chemicals: * 2 retort clamp and stand Ammonia * 1 ½ m glass tube * 2 250cm3 beakers * Cotton Wool * Stop clock * Meter
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beakers A dozen filter paper disks Test tube rack Hydrogen peroxide (H2O2) Potato extract Forceps Thermometer Hot plate Large beaker Ice cubes Graduated cylinder Stopwatch Procedure: Step 1 Place 10 mL of potato extract in a small beaker and check the temperature to ensure that it is at room temperature (20-25°C). Step 2 Using forceps dip a filter paper disk into the extract and drop it into the test tube with 10mL of hydrogen peroxide. Step 3 Allow the filter paper disk to sink to the
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Pre Lab Questions 1. What are the basic units of length‚ mass‚ volume‚ and temperature in the SI system? Length= meters (m) Volume= kilograms (kg) Temperature= Kelvin (K) 2. What decimal power do the following abbreviations represent: a) M=10^6 b) K=10^3 c) M=10 ^-3 d) N=10^-9 e) µ=10^-6 3. What is the number of significant figures in each of the following measured quantities? a) 351 g 3 b) 0.0100 mL 4 c) 1.010 mL 4 d) 3.72 x 10^-3 cm 3 4. What is the length of a crystal
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