Lab Report (Scientific Paper) 2: Bacterial Transformation;DNA Extraction Part I & II:Total Genomic Extraction & Plasmid Extraction;Electrophoresis By:Chris Foster Abstract: We conducted three experiments that included a Bacterial Transformation‚ a two process DNA extraction‚ and a final procedure using gel electrophoresis. The Bacterial Transformation lab was performed to prepare the plasmid into a bacteria and to use that bacteria to amplify the plasmid in order to make large quantities
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nucleotides) 4. In prokaryotes‚ DNA molecules are located in the c. cytoplasm (no nucleus) 5. The diagram below shows the process of DNA a. Replication 6. The main enzyme involved in linking individual nucleotides into DNA molecules is d. DNA polymerase 7. The process by which the genetic code of DNA is copied into a strand of RNA is called b. transcription 8. In messenger RNA‚ each codon specifies a particular c. amino acid 9. Changes in the DNA sequence that affect genetic information
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Figure 20.1 1) Which enzyme was used to produce the molecule in Figure 20.1? A) ligase B) transcriptase C) a restriction enzyme D) RNA polymerase E) DNA polymerase Answer: C Topic: Concept 20.1 Skill: Application/Analysis 2) Assume that you are trying to insert a gene into a plasmid. Someone gives you a preparation of genomic DNA that has been cut with restriction enzyme X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with
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Epigenetics: interaction of DNA methylation and chromatin Epigenetics is a field where advances are being made daily. Epigenetics is defined as “heritable changes in gene expression that occur without a change in DNA sequence‚” as stated by Dr. Alan Wolffe. A way in which we can understand this definition is by taking the analogy of a card game. The cards‚ the DNA sequence‚ have been dealt and will not change‚ however we need to understand how to play the cards‚ the rules‚ which is epigenetics
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Discuss the chain of events that led to the discovery of DNA as the heritable substance and the discovery of the structure of DNA. Ans> The chain of events led to the discovery of DNA as the heritable substance and the discovery of the structure of DNA. In the late nineteenth century‚ a German biochemist Friedrich Miescher found the nucleic acids which are long-chain polymers of nucleotides‚ and are made up of sugar‚ phosphoric acid‚ and several nitrogen-containing bases. Later it was found
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________________________ Student Exploration: Building DNA Vocabulary: double helix‚ DNA‚ enzyme‚ lagging strand‚ leading strand‚ mutation‚ nitrogenous base‚ nucleoside‚ nucleotide‚ replication Prior Knowledge Questions (Do these BEFORE using the Gizmo.) DNA is an incredible molecule that forms the basis of life on Earth. DNA molecules contain instructions for building every living organism on Earth‚ from the tiniest bacterium to a massive blue whale. DNA also has the ability to replicate‚ or make
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errors in DNA or from the harming impacts of mutagens‚ for example‚ chemicals and radiation‚ which respond with DNA and change the structures of individual nucleotides. All cells possess DNA-repair enzymes that attempt to minimize the number of mutations that occur. These enzymes work in two ways. Some are pre-replicative and look the DNA for nucleotides with irregular structures‚ these being replaced before replication happens; others are post-replicative and check recently synthesized DNA for mistakes
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formation which precedes gamete production in plants Haploid gametes (sperm ovum) - sexual reproduction DNA in a cell replicates only once‚ but cell divides twice The Cell Cycle Interphase G1: Protein synthesis and growth (10 hours) Preparation for DNA replication (e.g. growths of mitochondria) Differentiation‚ only selected genes are used to perform different functions in each cell S: DNA Replication (9 hours) G2: short gap before mitosis‚ organelles and proteins for mitosis are made (4 hours)
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Chapter 18 Chapter 19 Chapter 20 Chapter 21 Chapter 22 Chapter 23 Genetics: An Introduction...............................................................................................1 DNA: The Genetic Material ............................................................................................9 DNA Replication............................................................................................................17
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acid. ! C. Nirenberg - Deciphered first codon ! D. There is redundancy (multiple codons for one amino acid) but not ambiguity ! (one code specifies for two amino acids) ! E. Polyribosome - Clusters of ribosomes on same mRNA. III. Protein Synthesis ! A. DNA directs protein synthesis through RNA ! B. mRNA carries blueprint for a particular protein out of the nucleus. ! ! 1. Transcription - Copying of the genetic
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