BCH 369L–Biochemistry Laboratory Fall 2016 Module C Orlando Martinez uteid: olm298 Introduction The purpose of this lab is to implement the technique of gel electrophoresis in the purification and size determination of various proteins and DNA fragments. In order to do this‚ a polyacrylamide gel will be prepared and placed in a buffer-containing gel electrophoresis apparatus. Next‚ an aliquot of acid phosphatase and a molecular weight marker (Composed of Phosphorylase B‚ bovine serum albumin‚ ovalbumin
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was used to obtain colonies which were resuspended into a CaCl2 solution that was previously kept on an ice bath. The rainbow transformation mixture containing the plasmid DNA was then added to half of the E. coli cells. These cells were later placed into a water bath set to 42ºC and “heat shocked” to promote the entrance of DNA into the cells. Moreover‚ a Recovery Broth was added to the sample and the sample was left undisturbed for 30 minutes at room temperature so the “heat shocked” bacteria would
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couple of decades‚ biologists knew the nucleus contained DNA and proteins. The prevailing opinion was that the genetic material was proteins‚ and not DNA. The reason for this belief was that proteins are more complex than DNA. This is because A) proteins have a greater variety of three-dimensional forms than does DNA. B) proteins have two different levels of structural organization; DNA has four. C) proteins are made of 20 amino acids and DNA is made of four nucleotides. D) Only A and C are correct
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future is determined by their DNA. I wouldn’t want to live in this world‚ because your future and personal like/personality‚ you‚ are already chosen‚ so you have no choice of want you want your future to be like. In the movie GATTACA‚ Vincent has a dream to go to space but he can’t because of his DNA sequence. So he decides to become someone else in order to get a good job and fulfill his dream. Jerome has perfect DNA but he is disabled‚ so he agrees to give his DNA (skin cells‚ hair‚ nail cells
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Recombinant DNA Technology How to Mess with DNA for Fun and Profit OR OR How to Make a Mouse Glow in the Dark What the heck is Recombinant DNA? Recombinant DNA is what you get when you combine DNA from two different sources. For example: Mouse + Human DNA Human + Bacterial DNA Viral + Bacterial DNA Human + (other) Human DNA (It’s sort of like Frankenstein-DNA!) Why Make Recombinant DNA? Recombinant DNA Technology May Allow Us To: • Cure or treat disease • Genetically modify
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Essay on Evolution There are many mechanisms that lead to evolutionary change. One of the most important mechanism in evolution is natural selection which is the differential success in the reproduction of different phenotypes resulting from the interaction of organisms with their environment. Natural selection occurs when a environment makes a individual adapt to that certain environment by variations that arise by mutation and genetic recombination. Also it favors certain traits in a individual
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Chapter 20: DNA Technology Biotechnology: Use of living organisms to perform tasks. * Wine & cheese * Selective breeding * Antibiotic production * Recombinant DNA Restriction Enzymes * Bacterial enzymes: cut up foreign DNA * Specific: only but at recognition sequences * Palindromic: cut at the same base sequence on each strand‚ but in the opposite direction * The exposed bases provide “sticky ends” * H-bond to compliment bases of segments cut with same restriction
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detection is described in this study. The detection is based on competitive binding‚ where gold nanoparticles (AuNPs)‚ with average size of ~10 nm confirmed using UV-Vis‚ TEM and AFM images‚ are used as visualizing agents. These are bioconjugated with DNA which competitively binds with its complementary strand either in the sample or in the test line of the LFSB. The detection scheme reduces the number of probes which effectively lowers the cost for the design of the test strip. The whole test took less
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result of the fathers mitochondrial DNA being destroyed during the fertilisation. The mitochondria creates energy that powers more than 90% of the human body (United Mitochondrial Disease Foundation‚ 2012). Although for example red blood
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CHEM120 Week 7 iLab: DNA‚ mRNA‚ and Protein (30 points) Complete the two questions below. Each question has four parts. This assignment is two pages long. Question 1: For the following double-stranded DNA sequence‚ -CATTGACCGTAA- -GTAACTGGCATT- Answer the following questions: a) Assume that RNA polymerase will read the top strand of DNA as the “template” to synthesize mRNA. What will be the sequence of the mRNA synthesized? (3 points) The new mRNA sequence will be GUAACUGGCAUU
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