1. We isolated Vibrio fischeri DNA to pGEM genomic DNA using a phenol chloroform purification method. 2. Vibrio fischeri DNA and pGEM DNA was cut with restriction enzyme Sal I to prepare both for ligation. 3. Vibrio fischeri and pGEM DNA were ligated with T4 ligase. 4. E.coli DH5α competent cells were prepared for us so that transformation of the recombinant plasmids and the competent cells through heat shock transformation. 5. The transformation was then plated on LB/amp/X-gal plates. 6. The
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Nervous System and Sensory Organs • dorsal roots and ventral roots - connect Spinal nerves to the spinal cord • medulla -responsible for many involuntary functions such as heartbeat and breathing‚ primary communication pathway between the spinal cord and the rest of the brain‚ • cerebellum - receives input from multiple sensory receptor types and uses this information in coordination of complex body movements • pons- communication between lower and higher brain regions • midbrain- processes
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EXPERIMENT 1: PLATE CLUTCH | 1. INTRODUCTION Friction is to be avoided in some parts of machines‚ but is wanted in such items as clutches‚ brakes and belt-driven pulley systems. The apparatus used for this experiment is a simulation of a clutch‚ the pressure being provided by vertical weight on a plate. In engaging a clutch between a running engine and a stationary machine there is bound to be some wear‚ and it is evident that the wear must be uniform on the surface of the friction plate. As
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Under Microscope Lab Report A. The purpose of the investigation is to determine the time an onion cell spends in each part of the cell cycle. B. The significance of this investigation is to help us understand and know how long cells are undergoing mitosis. My personal interest is to know the phases of mitosis in cells in order to someday help find cures for cancer. C. The cell cycle is a chronological set of events‚ culminating in cell growth and division into two daughter cells. Some of the reasons
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Cell Surface Glycoprotein Receptor Analysis Using Concanavalin A Purpose: During this experiment we compared the hemagglutination reaction of control Con A solution at 2 mg/ml in Con A buffer with the hemagglutination reaction of your own purified Con A sample that you diluted previously at 2 mg/ml in Con A buffer. The purpose of this lab was to determine the strength of the reaction by performing serial dilutions on both the Con A sample and the control Con A sample‚ and determine through observations
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sin2 t + cos2 t = 1 Identities expressing trig functions in terms of their complements cos t = sin([pic]/2 – t) sin t = cos([pic]/2 – t) cot t = tan([pic]/2 – t) tan t = cot([pic]/2 – t) csc t = sec([pic]/2 – t) sec t = csc([pic]/2 – t) Periodicity of trig functions. Sine‚ cosine‚ secant‚ and cosecant have period 2[pic] while tangent and cotangent have period[pic]. sin (t + 2[pic]) = sin t cos (t + 2[pic]) = cos t tan (t + [pic]) = tan t Identities
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of certain insectivorous plants‚ e.g. Drosera‚ Dionaea the tentacles show variation in movements on coming in contact with an insect. The touch stimulus due to the insect is transmitted to the entire leaf and all the tentacles bend over the insect. 2/DIGESTION AND ABSORPTION=The small intestine is where the majority of food digestion occurs. At this point carbohydrates and proteins are already partially digested‚ while lipids have not yet begun. Chyme leaves the stomach and enters the duodenum (the
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Lab Report Experiment 1 & 2 CHM-101 Amele Takpara Partner: Jessamyn Dupree ------------------------------------------------- Experiment 1 ------------------------------------------------- (Pre-Lab Questions) 1. In the design of a Bunsen burner‚ explain the purpose of a. the gas control valve The gas control valve regulates the rate at which methane enters the burner. b. and the air vents. The air vents control the rate at which air enters the burner 2. Why is a luminous
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Unit 2: Earth Materials LAB: 2-3 Igneous Rock Identification Vocabulary: Igneous: Produced under the conditions involving intense heat. Intrusive: Igneous rock that forms beneath Earth’s surface. Extrusive: Igneous rock that forms at the Earth Surface. Felsic: Igneous rock rich in potassium feldspar and quartz and light color. Mafic: Igneous rocks that are rich in dark Plagioclase feldspar‚ pyroxene and
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Chapter 15 Managing Global Systems LEARNING OBJECTIVESS After reading this chapter‚ you will be able to answer the following questions: 1. What major factors are driving the internationalization of business? 2. What are the alternative strategies for developing global businesses? 3. How can information systems support different global business strategies? 4. What are the challenges posed by global information systems and management solutions for these challenges? 5. What are the issues and
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