Lecture 3: Enzyme kinetics Tue 17 Jan 2006 with the collaboration of Luna De Ferrari 1 Images from: D. L. Nelson‚ Lehninger Principles of Biochemistry‚ IV Edition‚ W. H. Freeman ed. A. Cornish-Bowden Fundamentals of Enzyme Kinetics‚ Portland Press‚ 2004 A. Cornish-Bowden Enzyme Kinetics‚ IRL Press‚ 1988 Computational Systems Biology Summary: • • • • • • 2 Simple enzyme kinetics Steady-state rate equations Reactions of two substrates Inhibition of enzyme activity pH
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Immobilization of Enzymes And Their Applications By‚ Shudhangshu Shekhar Kundu Introduction: What an enzyme? Enzymes are protein molecules‚ which serve to accelerate the chemical reactions of living cells (often by several orders of magnitude). Without enzymes‚ most biochemical reactions would be too slow to even carry out life processes. Enzymes display
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The bonding of an enzyme to its substrate forms an enzyme-substrate complex. The catalytic action of the enzyme converts its substrate into the product or products of the reaction. Each reaction is extremely specific‚ distinguishing between closely related compounds‚ including isomers. For example‚ the enzyme sucrase will only act on sucrose and will not bind to any other disaccharide. The molecular recognition of enzymes is due to the fact that they are proteins‚ which are defined as being macromolecules
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Factors Affecting Enzymes The activity of an Enzyme is affected by its environmental conditions. Changing these alter the rate of reaction caused by the enzyme. In nature‚ organisms adjust the conditions of their enzymes to produce an Optimum rate of reaction‚ where necessary‚ or they may have enzymes which adapted to function well in extreme conditions where they live.Increasing temperature increases the Kinetic Energy. In a fluid‚ this means that there are more random collisions between molecules
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Part 1 of 1 - 40.85/ 100.00001 Points Question 1 of 22 4.55/ 4.55 Points Record your answer from Lab Exercise #1‚ Step 1‚ Question 1.What time in hours:minutes:seconds GMT did the P waves arrive? A. 01:48:00 Correct B. 01:49:00 C. 01:50:00 D. 01:51:00 Answer Key: B Question 2 of 22 0.0/ 4.55 Points Record your answer from Lab Exercise #1‚ Step 1‚ Question 2. What time in hours:minutes:seconds GMT did the S waves arrive? A. 01:51:30 B. 01:52:00
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Enzyme-Controlled Reaction Procedure: Click the TV/VCR. Then click the Play button on the video controller. Watch an animation about enzyme action. Click More Information to read about enzymes and substrates. To conduct the experiment: Adjust the pH level of the test tube by click the up and down arrows Add substrate to each of the test tubes that already contain an enzyme solution Click and drag a piece of weighing paper with the powdered substrate to a test tube. Click the computer monitor to
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Eric Scantlebury NT1310 Physical Networking Unit 4 Lab 1: New building – Tools and Wiring 4/8/14 Now that we understand the codes and standards needed to create this building‚ it’s time to put stuff in it. Our task at hand is to start wiring the building for a new computer science and electrical engineering labs. This building will have a lot of devices that need to be connected to the school campus network and we will need a lot of tools to do it. First off I think since our building is going
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Lab 4 Projectile Motion Sai Moua Purpose: The purpose of this lab was to define what the initial velocity of the ball when it is launched out of the pipe. Our next objective is to determine at what angle that the ball will be ejected at the maximum range. Lastly‚ we predict and confirm the range before we launch the ball at a certain angle. Theory: Projectile motion according to Dr. James S. Walker is defined as‚ “the motion of objects that are initially launched –or “projected”- and that
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1. What makes Hot Topic so successful as a retailer? What makes them so popular with their employees? How can they keep their success going? Creating an environment were each employee regardless of their titles and positions share a common ground such as drive and passion to their products. Additionally their hiring modeling is to understand the age‚ diversity and cultural demographics and obtaining people that will understand the concepts behind their customer’s needs. Their motivation tactics
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Title: Catalase enzyme detection Objective: To understand the function of catalase in cells that produce the enzyme‚ interpret the results of a catalase test and know their value in differentiating bacteria. Materials: 1 clean microscopic slide‚ 3% H2O2 solution‚ swabs. Micrococcus luteus‚ Enterococcus faecalis‚ patient G Procedure: 1) Scrape some cells off from each bateria to the slant and place them on glass slide. 2) Place one or two drops of H2O2. Watch for bubbling as an indication of
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