"Lab 4 photosynthesis and chromatography of spinach leaves" Essays and Research Papers

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    The purpose of this lab was to investigate whether veins affect photosynthesis rate. It was predicted that having veins would decrease the rate because chloroplast might be less frequent due to the vascular tissue. For this experiment‚ 4 groups were set up: veins and no veins in CO2 solution‚ and veins and no veins in distilled water‚ the distilled water groups served as controls. These 4 groups were placed under a light bulb in order for photosynthesis to occur. No disks experience movement in the

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    Separation Of A Dye Mixture Using Chromatography ********* AP Chemistry (3rd Block) Fauquier High School 8/ 31/ 13 ABSTRACT: The Rf values of seven known color dyes and three unknown samples were observed in order to compare results and discover the hidden identities of the three samples. Three solvents‚ H2O‚ Isopropyl alcohol‚ and a 2% NaCl solution‚ were introduced to the dotted chromatography strips to show three different ratios for each dye on

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    Column and Thin Layer Chromatography Beverly Abstract: Plant pigments were separated and concentrated from a crude spinach extract through the use of column chromatography and an eluatropic series of hexanes‚ hexane/acetone‚ and methanol. The pigments were analyzed using thin layer chromatography with a 30% ethyl acetate/hexane developing solvent. Introduction: Chromatography is a technique used to separate a mixture of two or more components based on

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    The purpose of column chromatography is to separate one compound from another by the differing polarities in the compound. This technique is important in organic chemistry because certain experiments may require the separation of compounds in solution to be used for chemical synthesis and/or analysis of a particular desired product by isolating it. Fluorene is to be separated from 9-fluorenone using the technique of column chromatography. The success of separation and purity is determined with analyzation

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    relationship of different wavelengths of light and the rate of photosynthesis in spinach leafs. The rate of photosynthesis was measured every five min under light colors of white‚ green‚ red‚ blue and yellow under a light intensity of 2000 lux. The rate of photosynthesis was measured by the spinach disk method in which we replaced the air from the disks with sodium bicarbonate using a vacuum. Under photosynthesis‚ oxygen‚ a product of photosynthesis‚ replaced the bicarbonate solution‚ made the disks less

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    Problem: Why do leaves change color in the fall? Background: Leaves contain many pigments such as chlorophyll b‚ xanthophyll‚ and beta carotene besides chlorophyll a. They are part of the photosystem in the thylakoids. Pigment molecules can be separated by a procedure called chromatography. The procedure uses a special paper and solvent. The chlorophyll molecules adhere to the paper. The solvent molecules move up the paper by capillary action. Each chlorophyll molecule will travel up the paper at

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    Chromatography Analysis of Black Pens to Determine Unknown Sample Purpose: Paper chromatography was performed on five different black pens‚ using four different solutions to determine which would be most appropriate to use on an unknown sample. Paper chromatography was then performed on the unknown sample to identify which pen was used to create it. Procedure: Each person participating in the analysis was assigned one solution to work with: V. Temple used distilled water‚ D. Sellers used

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    Photosynthesis Lab Report

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    The majority of the experimentation in this project relied on the idea that there is a relationship between concentration and absorbance of a solution. This is easy to understand if you can visualize the particles in the solution; and this is especially true if the particles in solution portray a distinct color. The more particles there are in the solution‚ the more they will obstruct the path of the light. This occurs the most at the analytical wavelength‚ where the most light is absorbed by

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    Mobile liquid phase is always passed stationary phase during chromatography. Developed TLC plate is then observed under short wave ultraviolet light‚ where most TLC plates contain fluorescent mixed with silica gel that allow to glow green color. Compounds will absorb the UV light and appear as dark spot against the green color TLC plate. In a column chromatography‚ cotton and sand is placed in purpose of holding silica gel and prevent leakage of adsorbent particles

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    Photosynthesis Lab Report

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    Method: as in practical guide results: Experiment 1 calibration curve- Table showing the absorbance and concentration of the 10 dilutions of p-nitrophenol at 405nm Tube no  | blank  | 1  | 2  | 3  | 4  | 5  | 6  | 7  | 8  | 9  | 10  | 150 µM p-nitrophenol (ml)  | 0  | 0.1  | 0.2  | 0.3  | 0.4  | 0.5  | 0.6 | 0.7  | 0.8  | 0.9  | 1.0  | Bicarbonate buffer (ml)  | 1

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