ADI Lab investigation: Photosynthesis Introduction and goals: In our experiment we worked with photosynthesis‚ which is the process of light energy‚ CO2 and H2O being turned into food for the plant. CO2+H2O+ Light energy=C6H12O+O2 is the formula for photosynthesis. Our group hypothesis was trying to determine weather o2‚ water‚ or the nutrients of the soil determine the mass of the water. The guided question was “Where does the mass that makes up a plant come from?” Methods:
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CHROMATOGRAPHY _TOPIC_ : 7.2 _RESEARCH QUESTION:_ What is chromatography? How many pigments are there in a plant? How the different pigments in a plant can be separated? _APPARATUS_ : Please refer to the handout _MATERIAL_ : Please refer to the handout _METHOD_ : Please refer to the handout _DATA COLLECTION_: Coloured band Pigment front distance (± 0.05cm) Solvent front distance (± 0.05cm) Green 11.7 13.0 Yellow 12.5 13.0 _DATA PROCESSING_: Coloured band Pigment
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Figure 1: Affinity chromatography of fumarase with the Ni2+-NTA-agarose column. Extract (9.9 mL) containing yeast (3.76g) in extraction buffer containing 0.1% Igapel CA-630 and protease inhibitors were pumped through Ni2+-NTA-agarose column. Fractions were collected by 1.5 mL portions by use of wash buffer (20.0 mL)‚ imidazole elution buffer (26.3 mL)‚ and wash buffer (10.0 mL)‚ again. Absorption readings were taken for all fractions with a Cary50 set at 280nm. The fumarase activity was determined
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Photosynthesis is a crucial energy conversion process that occurs in the chloroplasts of eukaryotic cells. The rate of photosynthetic activity holds importance in environmental‚ horticultural and agricultural situations. (Boardman‚ N.K. 1977). Pigments in the chloroplasts thylakoid membrane absorb the electromagnetic radiation from a light source and release an electron into the electron transport chain. DCPIP’s chemical ability to favourably accept electrons‚ and undergo a colour change from
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PHOTOSYNTHESIS & CELLUAR RESPIRATION LAB REPORT % Absorption 400 425 450 475 500 525 550 575 600 625 650 675 700 Average 47 44 37 27 14 10 8 9 9 10 15 17 14 Use the graph of Average % Absorption for Grass Pigments to answer the following questions. ANSWER ONLY THE QUESTIONS BELOW. YOU WILL LOSE POINTS IF YOUR ANSWER INCLUDES UNRELATED INFORMATION. 1. What specific range of wavelengths explains why grass is green? The absorption of all wavelengths outside approximately
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Chromatography: How can we separate a mixture? Purpose The chromatography lab is to understand how molecules with similar molecular properties can be separated with paper chromatography. These differences will be interpreted to see the distinction of separate chemical substances. Pre Lab Questions 1. Explain capillary action as it pertains to water and paper. Capillary action makes water draw up the paper. As paper absorbs water mixes with the solutions in the paper. 2. What is the
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A Spectrophotometric Analysis of the Absorption of Green Light Versus Red Light Absorption in Spinach Leaves The goal of the experiment was to determine if green light had less ability to absorb than red light in spinach leaves. This was done by separating the photosynthetic pigments (chlorophyll a‚ chlorophyll b‚ carotene and xanthophylls) from one another using paper chromatography. The separated pigments were then analyzed for their absorption spectrum using a spectrographometer. When the
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Photosynthesis Lab Kozlowski Kendal Schweiss Hour 6 Introduction: Photosynthesis is carbohydrate production using light and chlorophyll. It is a process when green plants and other organisms turn carbon dioxide and water into carbohydrates and oxygen‚ using light energy trapped by chlorophyll. The rate of photosynthesis can be measured by either the disappearance of substrate or the accumulation of product. 2H2O + CO2 + light -> carbohydrate (CH2O) + O2 + H2O There is a spongy mesophyll
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OBJECTIVES: The objective of this experiment was to extract plant leaf pigments and determining them by using the Rf values obtained from the paper chromatography technique. The hypothesis of the experiment was that all of the five listed pigments would be present in the extracted plant leaf according to the Rf values. PROCEDURE/APPARATUS: The equipments used were a 18 x 150 mm test tube with stopper‚ graduated cylinders‚ Erlenmeyer flask‚ mortar and pestle‚ metric ruler‚ tall jar‚ acetone‚ tiny
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COLUMN AND THIN LAYER CHROMATOGRAPHY Maria Janine B. Abarientos‚ Kuia B. Allarde‚ Aliana Keshia P. Andino Mary Viadelle E. Andrada and Nina Marian Robelea G. Ang Group 1 2C Pharmacy Organic Chemistry Laboratory ABSTRACT In this experiment‚ the techniques column and thin layer chromatography was used to separate and determine the purity of the colored components of siling labuyo and malunggay leaves. The results obtained relied on differential solubilities and adsorptivities of the components to
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