Section 1 Purpose: The purpose of this lab was to separate plant pigments using chromatography‚ calculate Rf values using the collected data‚ and study photosynthesis with isolated chloroplasts. Light energy Light energy Background Information (Activity A): In photosynthesis‚ plant cells convert light energy into chemical energy that is stored in sugars and other organic compounds. It is an endergonic and anaerobic reaction. Critical to the process is chlorophyll‚ the primary photosynthetic pigment
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experiment consisted of three smaller experiments as a result of the different properties chlorophylls possess and the need to test them individually to receive the most accurate results. Preparation for the lab began with selecting a medium to large spinach leaf and deveining it. Once the spinach was prepared‚ it was ground in 5 mL of 100% acetone‚ creating a green acetone extract. The extract was filtered through a funnel with a kimwipe and into a test tube. The volume of the filtered extract was
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AP Lab #5 Plant Pigments/Photosynthesis I. Identifying the Effects of Different Variables of Light and Carbon Dioxide on the Rate of Photosynthesis and Observing the Separation of Pigments Through Chromatography II. Introduction Plants have a variety of pigments‚ all of which absorb a different color of light. The three main pigments are chlorophyll a‚ chlorophyll b and carotenoids. Chlorophyll a is the primary plant pigment that absorbs red and blue light‚ which ultimately appears green to the human eye
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Separation of leaves pigment. Introduction The photosynthetic pigment is used to absorb light during the process of photosynthesis. There are five pigments usually found in spinach leaves: Carotene (yellow)‚ Phaeophytin (yellow-grey)‚ Xanthophyll (yellow-brown)‚ Chlorophyll a (blue-green)‚ Chlorophyll b(green). Photosynthetic pigment is located in the chloroplast of the leaf. The function of chlorophyll a and chlorophyll b is to trap red and blue violet light when they carry out photosynthesis‚ chlorophyll
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I) Introduction In this lab‚ a test was conducted to determine how the relative redox activity of chloroplasts from spinach leaves‚ which were performing photosynthesis‚ would change when in the presence or absence of light. To observe these changes in redox activity‚ the chloroplasts were exposed to DCPIP‚ a chemical that changes color according to such activity. By determining the redox activity of the chloroplasts‚ it could then be inferred which chloroplasts were photosynthesizing more actively
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Photosynthesis is a process done in the chloroplasts of plant cells that is beneficial to all of life. Plants are able to convert light to energy which is beneficial to the plants‚ while also giving off oxygen as a byproduct for humans. Chloroplasts are in the thylakoid discs of a plant cell‚ that contain chlorophyll a and chlorophyll b‚ which are it’s light-capturing pigments. Colors in the wavelengths are either absorbed or reflected by the chlorophyll in which case green is reflected and colors
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What is Photosynthesis? Its general reference is sunlight protons converted into resourceful energy‚ of which are autotrophs that work and feed themselves independently. To be more scientific using its equation 6CO2 + 6H2O – Sunlight Energy – C6H12O6 + 6H2O‚ carbon dioxide particles travel through a leaf’s cell surface‚ which is where the chloroplast organelle produces “chlorophyll molecules forming a light harvesting complex absorbing that energy‚ exciting electrons” ((n.d.). Retrieved March 22
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Community Outreach & Education Program CHROMATOGRAPHY (Adapted from: Forensic Science Activities. University of Colorado Boulder Hughes Initiative. .) DESCRIPTION: Students will use paper chromatography to separate ink molecules and identify the pen used on an unknown sample of handwriting. Students will graph and analyze data they collect using paper chromatography. PURPOSE/GOAL: Students will be able to: • Gain understanding of the purpose of chromatography. • Measure and graph pigment separation
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Lab Report: Understanding Photosynthesis Gen Biology Lab Abstract: This lab was called photosynthesis: understanding photosynthesis. It is a highly complex process that needs to be broken down in many steps to understand how it works. This lab covers the big components in photosynthesis including carbon dioxide intake‚ light consumption‚ and varying pigmentation. Introduction: Photosynthesis is a huge concept to learn and understand in the field of biology. Plants have their own special
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take spinach leaves and extract the chlorophyll and carotenoid pigments by using acetone as the solvent. The chlorophyll and carotenoid pigments were extracted by using column chromography and alumina was used as the solvent. Solvents of different polarities were used‚ starting with the least polar‚ to extract the certain components from the leaves. They were then analyzed by using thin- layer chromatography. Procedure: The first part of the experiment dealt with breaking down the spinach leaves
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