The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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photosynthesis in Ivy disks Background: All plants are autotrophs meaning they create their own food from inorganic chemicals in their surrounding. In the case of food‚ plants use CO2 to create glucose. In addition to the inorganic chemicals‚ plants use sunlight to create their glucose because the photosynthesis reaction is endothermic. The rate of this reaction‚ in turn‚ can be measures. And this lab uses oxygen which is created as a result of the photosynthesis reaction. Since
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In paper chromatography‚ some compounds in a mixture travel almost as far as the solvent does; some stay much closer to the base line. The distance travelled relative to the solvent is a constant for a particular compound as long as you keep everything else constant. Some compounds may travel farther than others because they may be composed of more components‚ thus traveling farther. For example‚ black is composed of every color of the rainbow‚ while yellow is composed of less colors. As a result
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The rate of photosynthesis in each of the beakers will depend what is wrapped around the beakers such as red and blue cellophane‚ as well as the control of the experiment the beaker without cellophane. The cellophane will directly impact the light getting absorbed as well as the amount of CO2 in the beakers. Which overall as result will determine the final color of the water mixed with bromothymol blue. What was being measured was the color of the water after CO2 has been blown in the beakers. The
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experiment is called chromatography. The materials used consisted of – 3 pieces of chromatography paper‚ a tall clear glass‚ water‚ table salt‚ 1 tsp. measuring spoon‚ an 8 oz. measuring cup‚ pencil‚ ruler‚ 6 washable markers‚ and paper towels. The markers used in this experiment have to be water soluble therefore I used washable markers made by Crayola. The colors I chose were brown‚ teal‚ red‚ orange‚ black‚ and green. The salt solution served as the mobile phase while the chromatography paper served
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Introduction: Photosynthesis is a process to which some autotrophs such as plants produce their own food. It has two stages or reactions light-dependent and light-dependent reactions. The light dependent reactions are the first stage‚ where energy from sunlight is captures in Photosystem 2 and then 1‚ the electrons generated in Photosystem 1 then moves along the electron transport chain. The moving of electrons causes a hydrogen ion gradient that is used in the final step to produce ATP‚ by the
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Data and Conclusions: The purpose of this experiment was to learn how to use distillation and gas chromatography to separate and identify different compounds from a given mixture. There are several kinds of distillation methods. However‚ the method that we used in this experiment was fractional distillation. This method is used when trying to separate two different volatile compounds whose boiling points differ by 40-50°C or more. If the boiling points are too close‚ this method
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Introduction Photosynthesis is a redox reaction which requires carbon dioxide‚ water and light to produce water and a 6-carbon sugar. The process of photosynthesis consists of two parts‚ a light reaction and a light-independent reaction. The method of changing light energy into chemical energy for the formation of NADPH and ATP is done through the light reactions. Light independent reactions use carbon dioxide and the products of light reactions (ATP and NADPH) to form compounds such as glucose
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_______(savanna chatham)_________________ Virtual Lab #5- “Which Colors of the Light Spectrum are Most Important for Plant Growth? Instructions: Open the link to Launch “Virtual Lab #6- Photosynthesis” Read all instructions on the left-hand side of the lab. Watch the short Video at the bottom of the lab Complete the “Pre-Lab” Questions. Complete the Lab Activity Record your findings in the “Results” and “Post-Lab” sections and answer all review questions Pre-Lab: 1.) List all of the colors found in the visible
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The purpose of the experiment is to determine the effects of sugar on photosynthesis in Elodea. Sugar water with Elodea‚ water with Elodea‚ and water (control) are used to test for photosynthesis. Five drops of sugar water were added to sugar water with Elodea beaker. The three beakers were left under normal light for photosynthesis to occur. After 60 minutes‚ NaOH solution was added to each beaker to test the volume of CO2. The result shows that both Sugar water with Elodea and water with Elodea
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