objective of these labs were to use sophisticated techniques to produce double-stranded RNA that was incubated with live Drosophila cells to inhibit the expression of our two genes of interest. The overall process of the four labs was to isolate and amplify DNA using the polymerase chain reaction with primers that contained gene-specific sequences to Thread or Dynamin-related protein 1 (drp-1)‚ along with T7 promoter site sequences. The amplified DNA was purified and both strands of the DNA were used as
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Experiment 1 Synthesis of Acetaminophen Clifford Tse 20529845 Partner – Raminder Dhanoa TA – Xiao Qi CHEM 123L - 012 February 6‚ 2014 Introduction Acetaminophen‚ also commonly known as Tylenol‚ is an over-the-counter medicine used to relieve pain and reduce fevers. Within this experiment‚ Acetaminophen will be formed through the reaction between p-aminophenol and acetic anhydride. This reaction will incur Acetaminophen as a crude solid being impure‚ which will be purified further through
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Determining Allele Frequencies of the PV92 Alu Element using DNA Isolated from Human Cheek Cells and PCR Amplification Background Alu elements are the most abundant repetitive elements in the human genome that have mobilized throughout primate genomes by retrotransposition over the past 65 million years ago from a 5’ to 3’ fusion of the 7SL RNA gene‚ to reach the present number of more than one million copies. Over the last few years‚ several lines of evidence demonstrated that these elements
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The Grignard Synthesis of Triphenylmethanol Abstract: Grignard synthesis of triphenylmethanol was achieved by use of the Grignard reagent phenyl magesium brominde. The organometallic grignard reagent was synthesized by use of a reflux apparatus recrystallization techniques. Once synthesized it was used in a Grignard reaction that involved nucleophilic addition to a carbonyl in order to make triphenylmethanol. The final product was solidified and recrystallized and spectral data was
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Vectors‚ Sections 3.1-3.2 Chapter 5 Two Dimensional Kinematics‚ Sections 5.1-5.2 Chapter 6 Circular Motion‚ Sections 6.1-6.3 Tuesday Sept 10 Problem Set 1 Due 9 pm Sept 11/12 W02D02 Applications of Newton’s Second Law Reading Assignment: Chapter 7 Newton’s Laws of Motion‚ Sections 7.1-7.4 Chapter 8 Applications of Newton’s Second Law‚ Sections 8.1-8.4.1 Sept 13 W02D3 Problem Solving 01: Applications of Newton’s Laws Reading Assignment: Chapter 8 Applications of Newton’s Second Law‚ Section
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extract DNA from a strawberry using limited household items. This was accomplished with the prior knowledge that the DNA is stored in the nucleus of the cell‚ and that is an extraction buffer would be needed to acquire the DNA. This experiment had no control group‚ however‚ if there was a control group it might be a group with no extraction buffer. The independent variable in this experiment is the extraction buffer. The dependent variable in this experiment is the ability to extract DNA. The constants
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The upper most strand is the (coding strand) DNA base sequence (triplet) of the gene codes for synthesis of a particular polypeptide chain. The second strand is the mRNA base sequence (template strand used for copying) codon of the transcribed mRNA. The process for going from the upper stand to the second strand is called Transcription and involves an enzyme called polymerase. The polymerase attaches to the promoter region (start codon) and reads the nucleotide base sequence until it gets to a termination
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Protein Monique Rowland Nutrition / 241 Instructor: AN-CHIAN CHEN October 4‚ 2013 Protein All proteins are molecules that are the boosters of living organisms and are part of each organ‚ tissue‚ and cell within the human body. The protein in consumed food turns into amino acid. The amino acid is used later replinish the proteins used. Proteins have two structures. the first and primary structure of protein is the sequence of the amino acid in the protein (Kim‚ 2005). The amino acid
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mL of unknown #12. The flask was then covered and had a hole poked through the foil. Next‚ it was placed into boiling water. After the liquid was fully evaporated it was taken out‚ wiped dry and‚ allowed to cool. Then the mass was measured. Then the lab station was cleaned and the waste properly disposed of.
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Tie-Dye Grignard Synthesis Abstract: 4-Bromo-N‚N-dimethylaniline underwent a Grignard reaction with diethyl carbonate to produce a type of the tie-dye chemical triarylmethane. This specific triarylmethane produces a vivid crystal violet color when dyed. The experiment was first heated under reflux to produce the necessary Grignard reagent as a grey liquid. It was then reacted with diethyl carbonate and hydrochloric acid to produce crystal violet. The resulting chemical was very absorbent to
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