In this lab we employed various assays utilizing a biuret reagent‚ coomassie brilliant blue reagent‚ and ultraviolet light in order to determine the identity of six unknown solutions and the concentration of a bovine serum albumin sample. We were given three samples that lacked protein‚ and three samples containing proteins‚ and using a spectrophotometer we assessed the amount of light absorbed versus the light transmitted‚ based on the principles of the Beer-Lambert Law. The three proteins used included
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Protein synthesis is one of the most fundamental biological processes. To start off‚ a protein is made in a ribosome. There are many cellular mechanisms involved with protein synthesis. Before the process of protein synthesis can be described‚ a person must know what proteins are made out of. There are four basic levels of protein organization. The first is primary structure‚ followed by secondary structure‚ then tertiary structure‚ and the last level is quaternary structure. Once someone understands
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Protein synthesis is the process whereby proteins are produced‚ or synthesized‚ in living things according to "directions" given by DNA (deoxyribonucleic acid) and carried out by RNA (ribonucleic acid) and other proteins. As suggested earlier‚ this is an extraordinarily complex process that we do not attempt to discuss here. Following synthesis‚ proteins fold up into an essentially compact three-dimensional shape‚ which is their tertiary structure. DNA contains the instructions for a cell’s structure
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Identifying sites of protein synthesis in Chlamydomonas using erythromycin and cyclohexamide as protein synthesis inhibitors. October 16‚ 2009 Introduction: In living cells‚ prokaryotic or eukaryotic‚ the synthesis (construction) of proteins is accomplished by similar machinery. Amino acids‚ ribosomes‚ messenger RNA (mRNA)‚ and transfer RNA (tRNA)‚ are all necessary for the building of functional proteins in a cell. Ribosomes are the site of protein synthesis in a cell‚ and there are two
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DNA and the Gene: Synthesis and Repair 1) Watson and Crick elucidated the structure of DNA in 1953. Their research built on and helped explain the findings of other scientists‚ including ________. A) X-ray diffraction studies by Rosalind Franklin and Maurice Wilkins. B) Chargaff’s rules: C = G and T = A. C) Scientists who recognized that a nucleotide consisted of a sugar‚ a phosphate‚ and a nitrogen-containing base. D) All of the above were important considerations in the elucidation of
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see looking at the Total Protein column on Table 3‚ the most effective step with regard to the percent of remaining protein removed was affinity chromatography because it was able to remove 98.6% of the remaining proteins. In comparison to 81.93% removed during the 65% ammonium sulfate precipitation and 81.3% during the size exclusion. This means that the affinity chromatography removed a big percentage of contaminating proteins. However‚ removing this huge amount of protein left us with a small amount
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In order to read DNA‚ it must be sequenced. This sequencing uses electrophoresis‚ a technique that separates sections of DNA that differ by a base. Electrophoresis used to be done manually‚ but was error prone and time consuming. Now‚ automatic sequencing machines are used. A technician begins the process by pouring gel between two glass plates that are set less than half a millimeter apart. After the gel is set up‚ DNA is put into each of the ninety-six lanes. The DNA sections then move through
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Review 2: Text DNA is a very special and important molecule that is in our life‚ DNA is found in the nucleus. DNA stores the data needed to make poly peptides‚then data in a nucleic acid is transferred to messenger RNA‚ which escapes the nucleus into a protein. According to the DNA and protein article DNA is used in many things but DNA is used to make pol-peptides. They are another word for proteins. When a cell makes a protein the direction are resembled from the part of an DNA. DNA strand and transcript
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Protein Synthesis Worksheet 1. 2. a. Protein synthesis began in the nucleus. b. Chromatin is a diffuse mass located in the nucleus where genetic material is found. c. A gene is a sequence of nucleotides‚ it is important because they form the template and these templates are used for synthesizing proteins. 3. a. Transcription is the first step of protein synthesis it uses a sequence of DNA nucleotides to produce a sequence of RNA nucleotide. b. RNA polymerase bonds to RNA nucleotides together
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Protein Synthesis Notes If DNA’s shape is altered it can’t function properly. RNA is more flexible so changing the shape changes the function‚ which is wanted. Any shape of RNA can become another. Three shapes/functions of RNA: -mRNA: messenger (linear_____) -rRNA: ribosomal (large and like a globe) More stable than mRNA. -tRNA: transfer (shaped like t) If you flatten this it becomes mRNA. Hydrogen Bonds ………………………………. Protein Synthesis Overview- Two Processes: Transcription- synthesis of
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