2014 Biology 1 Cellular Processes Lab Section 903 Tianna Clarke Materials and Methods Part I – Restriction Enzyme Digestion To begin this experiment‚ the DNA molecules must be cut into smaller fragments with distinct enzymes called Restriction Enzymes through a process called Restriction Enzyme Digestion. Four microtest tubes were labeled 1 through 4 and added 10 µl of Enzyme Reaction Buffer to each of the four reaction tubes using a micropipette. DNA‚ and Enzyme 1 and 2‚ were then added to the
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This lab is adapted from the canned denatured pineapple experiment. Instead of investigating the effect of canning on enzyme activity‚ we look for the presence of the substrate. This is a useful teaching lab for all the criteria and requires very few resources. Background Information: Gelatin is made from a protein called collagen which comes from the joints of animals. Gelatin may be dissolved in hot water. As the dissolved gelatin mixture cools‚ the collagen forms into a matrix that
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Name __________________________________ AP Biology Period _________ Date ______________________ AP: LAB-RELATED AP EXAM ESSAYS LAB 1. OSMOSIS AND DIFFUSION ESSAY 1992 A laboratory assistant prepared solutions of 0.8 M‚ 0.6 M‚ 0.4 M‚ and 0.2 M sucrose‚ but forgot to label them. After realizing the error‚ the assistant randomly labeled the flasks containing these four unknown solutions as flask A‚ flask B‚ flask C‚ and flask D. Design an experiment‚ based on the principles of diffusion
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Jennifer Anatomy and Physiology Enzyme Lab Report Introduction The chemical reaction that is being studied is the hydrolysis of starch. The enzyme that is being studied is amylase. This experiment is looking at the effect of temperature on the rate of the enzymatic hydrolysis of starch. My hypothesis is that the higher the temperature the faster the hydrolysis of the starch would occur. The rationale behind this is that heat
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Question 1: The sequence in the entry that was obtained from sequencing a piece of DNA from Vibrio fischeri genomic DNA digested with Sal I is 8654 bp long. Question 2: The -10 sequence for the lux operon is tgttata. 2 nucleotides are different when compared to the ideal sequence. Question 3: The lux R has its own promoter and is transcribed in the opposite direction from the lux operon‚ so it cannot be transcribed from the same strand because the RNA Polymerase recognizes promoter sequences
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Biology “Enzyme Activities” Introduction: Enzymes have extremely interesting properties that make them little chemical-reaction machines. The purpose of an enzyme in a cell is to allow the cell to carry out chemical reactions very quickly. These reactions allow the cell to build things or take things apart as needed. This is how a cell grows and reproduces. At the most basic level‚ a cell is really a little bag full of chemical reactions that are made possible by enzymes (Brain). Laboratory
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Introduction Enzymes are protein based structures that help speed up chemical reactions. They help these reactions keep up with the everyday metabolic needs and other like functions of organisms. Enzymes are also considered catalysts‚ due to the lowering in activation energy‚ in which they are not consumed or changed at any point during the reaction. These enzymes have three main protein structures that help keep them formed and intact. Stage 1 of these structures is the primary structure‚ which
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Working Towards Wellness Assignment Instructions To complete this assignment: 1. Fill in the information above. 2. Answer all of the questions below; the boxes expand to accommodate your writing. 3. An example of an excellent response is shown below. 4. Directions for saving and submitting can be found at the end of this document. Objective: Demonstrate appropriate self-care skills including health maintenance and stress management. Example Question: How do you think wellness
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two Title: Enzyme Function Purpose: To observe the role of enzymes in chemical reactions and to determine the kinds of cells that contain more of the enzyme catalase. Prior Knowledge: Enzymes are proteins that assist the chemical reactions of a cell by lowering the amount of activation energy needed to start the reactions‚ thereby enabling the cell to carry out the reactions at a faster rate; enzymes that lower the activation energy are therefore called catalysts. Moreover‚ the enzyme itself is
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What is Enzyme? Enzymes are protein that acts as speed up reactions and break down molecules in our body. However‚ different enzymes only work on certain types of molecules. Enzymes can accelerate the reactions by more than one million times.(3) In our human body‚ there are a total about forty thousand types of enzymes and each catalyzes different kind of molecule.(3) The molecules that enzymes help to accelerate is called substrates‚ and when enzyme is combined together with the substrate‚ it
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