Objective: The objective of the lab is to separate the caffeine samples we are using‚ using thin layer chromatography. The solvent we are using for the separation is 3:1 mixture of Chloroform and Acetone. Principle: Thin layer chromatography (TLC) is an important technique that is useful for separating organic compounds. TLC is often used to monitor the progress of organic reactions and to check the purity of products. Separations in Thin layer chromatography involve distributing a mixture of
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Computer Lab Rules & Regulations Electronic workstations may only be used by current Swinburne University students and staffs. Swinburne identity card must be presented on request. Swinburne computing facilities should only be used for educational‚ research and administrative purposes of Swinburne. All other uses are strictly prohibited. The following rules and terms apply to all computers on campus. Terms and Conditions 1. All users must abide by the license requirements of any software
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Drugs screen using Thin Layer Chromatography of basic illicit drugs Abstract The majority of evidence submitted to crime labs comes from drug-related crimes. Often‚ this evidence includes unidentified powders that may be illegal drugs. In order to prosecute individuals for possession of illegal substances‚ it is necessary for forensic scientists to positively identify any suspected drugs submitted to the laboratory. In addition‚ forensic toxicologists must determine the identity
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CHM130 Lab 9 Chromatography Name: Karlee Rose A. Data Table (12 points) Paper # Color Source Solvent Distance Component Moves Distance Solvent Moves Rf value 1 Yellow M&M Candies 0.1% Salt Solution 28.88mm 42mm 0.69 2 Yellow Reese’s Pieces 0.1% Salt Solution 16.95mm 32mm 0.53 3 Purple Grape Soda 0.1% Salt Solution 32.15mm 51mm 0.63 4 Purple Grape Koolaid 0.1% Salt Solution 12.12mm 31mm 0.39 5 Red Easter Egg Dye 0.1% Salt Solution 1.18mm 7mm 0.17 6 Red Dry Erase Marker
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Chromatographic conditions Shimadzu VP-ODS column is (250 mm x 4.6 mm i.d.‚ 5 µm particle size)‚ Shimadzu‚ Kyoto‚ Japan. The mobile phase consisted of a mixture of 0.3% TEA‚ 10% n-propanol in a solution of 0.1 M SDS adjusted to pH 2.8 using 0.02 M orthophosphoric acid. It was pumped at a flow rate of 1 mL min-1. The column was kept at ambient temperature and the wavelength of the detector was at 225 nm. Standard solutions Stock solutions of LVD‚ CBD and ETC containing to 1.0 mg mL-1 were prepared
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Lab BCH 2333 Section: Lab 1 Carbohydrates: Separation Techniques Based on Molecular Size TA: Wednesday‚ January 16th‚ 2013 Team #4 By: Partner: Purpose The purpose of this experiment is to exemplify how differences in molecular weight allow separation of polymers from their monomers. Methods of dialysis and gel filtration chromatography will be used to separate a glucose monomer from a starch polymer. Colorimetric
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gain from the pictures taken at the scene? The v-shaped pattern often occurs in the area where a fire originated‚ so it is possible that the space heater was the fire of origin (tests needs to be run to confirm). The crazed glass (by lab results) indicated that crazing is the result of rapid cooling of hot glass by the application of water. The protected surface shows there was an object on the floor protecting the area‚ and there is the shape of a body that was there during the fire
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Extraction Important Peaks Concluded Compound C-5 #1 -CH‚ C=O cyclopentanone C-5 #2 -OH‚ -CH 1-hexanol D-4 #1‚2 C=O‚ -CH Cannot conclude Preparative Gas Chromatography was used to separate volatile compounds‚ which can then be identified. The injected sample becomes vaporized and was carried through the mobile column. It is usually carried by an inert gas. The gases were moved through the stationary column. The polarity of the components to the column would cause the separation of the components
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NATIONAL UNIVERSITY OF RWANDA FACULTY OF APPLIED SCIENCES DEPARTMENT OF COMPUTER SCIENCE BACC IV ACADEMIC YEAR: 2012-2013 CHAPTER 1. TOPIC: DESIGN AND IMPLEMENTATION OF AN ONLINE COMPUTER LABS MONITORING SYSTEM CASE STUDY: NATIONAL UNIVERSITY OF RWANDA ICT CENTER/Helpdesk Unit
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Proteins (ACTIVITY 8) Gallemit‚ Lacei Machelle• • January 17‚ 2013 ABSTRACT Chromatography is one of the most useful methods of separating minute amounts of complex mixtures into the pure substances contained therein. Chromatographic separations are effected by allowing the components in the mixture to be distributed in two phases‚ one being stationary‚ and the other‚ mobile. Partition chromatography involves two main distribution systems‚ one system is composed of a gas and a liquid and
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