TLC FULL LAB REPORT Objective: The purpose of this experiment was to identify compounds in a mixture by Rf values and to determine the best solvent to use. Also is the analysis of mixtures before‚ during and separation. Possible solvents: Hexane MW- 86.17 g/mol Hazards-flammable‚ harmful if swallowed Melting pt / boiling pt (degree Celsius) - 69 Density-.6548 g/ml [pic] Methanol MW-32.04 g/mol Hazards- flammable‚ eye irritant Melting pt / boiling pt- 65 Density-.7918 g/ml [pic]
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Lab 4: Photosynthesis and Chromatography of Spinach Leaves Discussion: The chromatography of the spinach leaves was taken to establish what pigments were present in the chloroplasts of the leaves. The solvent carried and separated the different pigments up the cellulose paper. The solvent moved up the paper because of capillary action because of adhesion of the solvent molecules and the paper. The pigments dissolved in the solvent also were carried up the paper and were separated because
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solutions: Lab report: outline Ink chromatography Chromatography is the separation of a mixture by passing it in solution or suspension or as a vapor (as in gas chromatography) through a medium in which the components move at different rates. It is used to separate substances. Examples of chromatography are finger-printing‚ medicines‚ and separating/ testing histamines. http://www.biotecharticles.com/Agriculture-Article/Applications-of-Chromatography-1299.html The purpose of this lab is
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The purpose of the lab “All in the Family” was to compare the reactivity of the halogens Chlorine‚ Bromine‚ and iodine by observing the reactions between their elemental forms and their ionic forms. To accomplish this experiment‚ we first added a squirt of pet ether to two test tubes. Then‚ we added the same amount of Cl2 to both of the tubes‚ shook the mixtures‚ and recorded the color that resided in each mixture as the initial color. After‚ a squirt of NaBr was added to one test tube‚ and a squirt
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OBJECTIVE The purpose of this experiment is to analyze mixtures of compounds prior to‚ during and after a separation scheme. This experiment also allows monitoring reactions of organic molecules‚ and determines the identity of a mixture of compounds. STRUCTURES AND PHYSICAL PROPERTIES OF REACTANTS [1] SOLVENTS | a. Hexane1Molecular Molarity: 86.18 g/molBoiling Point: 69 ºCMelting Point: -95ºCDensity: 0.659 g/mL at 25ºCWater Solubility: Insoluble in waterColor/Texture: Colorless/LiquidHazardous
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of the GFP protein. This indicates that the molecule responsible for the observed green trait was protein. The transformation efficiency was found to be 793.75 transformants per microgram. The protein was filtered out of the cell and column chromatography was used as a means to isolate the protein by taking advantage of its hydrophobic properties. This was performed by washing the column containing the GFP protein amongst other proteins with buffers varying in salt concentrations. As expected
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composition that was found out in the analysis. The % composition of the yield was found out by using the % composition of the products and a gas chromatography by measuring the peak areas for each gas chromatogram. The operations used in this lab in their order of usage were: Simple distillation‚ mixing‚ washing liquids‚ drying liquids‚ gas chromatography. 2. Procedure The volume of 20 mmol of 2-methylcyclohexanol was calculated and transferred into a round-bottom flask. Then 0.7 mL of 85 % phosphoric
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Introduction Chromatography is a technique used to separate a mixture into its individual components. In this experiment‚ chromatography is done by using a thin layer chromatography (TLC) plate and filter paper. The word ‘chromatography’ itself means the separation of colours. However‚ nowadays‚ both coloured and colourless compound are used in chromatography by various methods. In chromatography‚ the separation process of mixture is mainly based upon the polarity and molecular weight of sample
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Forensic Chemistry and Forensic Chemist Forensic chemistry is becoming an increasingly popular topic. It is being used quite often in the real world with police investigations‚ cases‚ and is also being magnified in television shows including Forensic Files‚ CSI‚ and Bones (What is Forensic Chemistry?). Forensic chemistry is important because without it we wouldn’t know the outcome of a crime. The forensic chemist’s job is to examine evidence given to them from a crime scene‚ when it happened‚ and
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Sample Lab Report Simple and Fractional Distillation Unknown # 2 Purpose In this experiment we aim to demonstrate that we can separate two volatile compounds from a mixture due to the different chemical properties of each compound. We will accomplish this by a separation procedure known as distillation‚ which relies on each compound having a distinct and separate boiling point. Our pure products will be analyzed with gas chromatography to determine the success of the distillation. Procedures
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