Post lab Questions 1. 3-decanone will have a higher Rf value than 3-decanol‚ toluene will have a higher Rf value than benzoic acid‚ and cyclooctane will have a higher Rf value than cycloctanone. This is because they are not as polar as the compounds with lower Rf values‚ the compounds that are most polar tend to stay longer with the polar adsorbent‚ while the least polar compound travels with the eluent that is lower in polarity. 2. TLC in today’s experiment stands for Thin Layer Chromatography
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Fractional Distillation & Gas Chromatography Exp. 1 Pre-Lab: 1) When two substances whose molecules are very similar from a liquid solution‚ the vapor pressure of the mixture related to vapor pressure of the pure substance. Also it could be defined as a two liquid are ideal solution when they don’t react with each other and they make no association. 2) Are a mixture of at least two different liquid‚ and known also as a mixture of two or more liquid in such away that its component
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RADICAL HALOGENATION AND GAS CHROMATOGRAPHY Abstract In radical halogenations lab 1-chlorobutane and 5% sodium hypochlorite solution was mixed in a vial and put through tests to give a product that can then be analyzed using gas chromatography. This experiment was performed to show how a radical hydrogenation reaction works with alkanes. Four isomers were attained and then relative reactivity rate was calculated. 1‚1-dichlorobutane had 2.5% per Hydrogen; 1‚2-dichlorobutane had 10%; 1‚3-dichlorobutane
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Chlorophylls and Beta Carotene from Plant Leaves Wang Haina A0133901R 1. Aim 1. To isolate chlorophyll and beta carotene from plant leaves using column chromatography. 2. To qualitatively characterise the pigments with UV-vis spectroscopy and TLC. 2. Results and discussion Isolation of beta carotene and chlorophylls by column chromatography Upon the loading of S1 (the extract of the plant leaves in hexane)‚ a yellow band appeared at the top part of the silica column immediately after the solvent
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Date: 9-10-13 Title: HPLC analysis of Caffeine Lab Partners: Objectives: 1) The main objective of this lab is to gain experience in using and reading results from the HPLC machine. 2) The first step is to inject a series of caffeine standards into the machine in order to get results/values of peak height and area. 3) The next step is to investigate the effects of a series of HPLC parameters on Retention time‚ peak height and peak area. 4) To use above data from peak height and area
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Candy Chromatography By: Avani Reddy 7th Grade Introduction Ever wondered why candies are different colors? Ever wondered why candies are different colors? Many candies contain colored dyes. Bags of M&Ms or Skittles contain candies of various colors. The labels tell us the names of the dyes used in the candies. But which dyes are used in which candies? We can answer this by dissolving the dyes out of the candies and separating them using a method called chromatography. Research
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Abstract In order to experiment with distinct components of a mixture‚ they must be first separated so they can be observed individually. This is accomplished in this lab by the technique called thin layer chromatography (TLC). TLC involves a stationary phase‚ which the TLC plates as well as a mobile phase‚ which could be one of two solvents used: ethanol-acetone for TLC. Dyes in a sample separate consequently because of their unique polarities. As a result‚ nonpolar substances travel further
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random process * Systematic – * Stratified-Random – division into parts‚ layers * Cluster – divided into subgroups * Composite – two or more samples are combined 3. Mixed – combining other sampling scheme Types of samples taken in Lab: * Raw mat * Processed control sample * Finished products * Buying samples Methods used in DISINTEGRATION: * Mechanical > tools * Ball mills * Meat mincers * Tissue grinders * Enzymatic and Chemical CHARACTERISTICS
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Chromatography of Commercial Analgesics Chromatography of Commercial Analgesics CHEMISTRY 200L EXPT 4 PAGE 8 - 11 CHEMISTRY 200L EXPT 4 PAGE 8 - 11 Janna Vernice R. Villalon*‚ Christian V. Villanueva‚ Cyd Vincent L. Zamora Department of Chemistry‚ College of Science *Corresponding author; e-mail: janna.villalon@rocketmail.com Abstract In analyzing the chromatography of analgesics‚ thin layer chromatography (TLC) was used. A very thin (micron) film of silica is coated on a glass
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Performed on: Wednesday‚ May 8th‚ 2013 | | | 5/15/2013 | | Introduction: The goal of this experiment was to identify an unknown analgesic drug through the use of thin-layer chromatography. The drugs used were‚ Unknown #1‚ caffeine‚ aspirin‚ and acetaminophen. Thin-Layer Chromatography is a technique for identifying substances using a mobile phase (developing solvent) that draws up through a stationary phase (TLC plate) and marks spots of differing distances for differing substances
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