experiment is to identify an unknown proprietary drug using thin-layer chromatography. The unknown’s behavior in thin-layer chromatography will be compared with that of its possible component analgesics. The possible unknowns and their analgesic ingredients will be Anacin (aspirin‚ caffeine)‚ Excedrin (acetaminophen‚ caffeine‚ aspirin)‚ Motrin (ibuprofen)‚ and Tylenol (acetaminophen). Introduction Thin-layer Chromatography(TLC) was the method used to figure out and identify the drug. TLC is done
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Crime scene report Introduction A suspected white powder was seized from the crime scene and sent to the laboratory for analysis. Presumptive test was firstly used in order to find out if it was a positive drug‚ in order to follow a further analysis. Gas Chromatogram (GC) and mass spectrum was used for further analysis‚ in order to find out what exactly was this drug‚ and find its mixtures and compounds. Results From the chromatogram/spectrum peak report it is shown that there are two retention
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Introduction In order to understand what HPLC‚ that is‚ High Performance Chromatography‚ it is necessary to understand the meaning of the term chromatography itself. Chromatography Chromatography is defined as a procedure by which inert materials and drug principles encountered in pharmaceuticals preparations are separated by fractional extraction‚ adsorption‚ or ion exchange on a porous solid. Chromatography is particularly useful as a means of separating and purifying complex and closely
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Student Number: xxx Class: xxx Aim The aim of this experiment was to determine the concentration of aspartame in a sample of diet Coke® using HPLC as investigation tool. Introduction High Performance Liquid Chromatography (HPLC) is one of the most widely used analytical techniques of nonvolatile organic compounds. Chromatographic processes can be described generally as mass-transfer between stationary and mobile phase. In HPLC‚ a liquid mobile phase is used to
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High-performance liquid chromatography (formerly referred to as high-pressure liquid chromatography)‚ HPLC‚ is a chromatographic technique used to separate the components in a mixture‚ to identify each component‚ and to quantify each component. HPLC is considered an instrumental technique of analytical chemistry (as opposed to a gravimetric technique). In general‚ the method involves a liquid sample being passed over a solid adsorbent material packed into a column using a flow of liquid solvent.
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CHROMOTOGRAPHY Chromatography is used to separate mixtures of substances into their components. All forms of chromatography work on the same principle that they all have a stationary phase (a solid or a liquid supported on a solid) and a mobile phase where liquid or a gas is involved. The mobile phase flows through the stationary phase and carries the components of the mixtures with it. Different components travel at different rates. In paper chromatography‚ the stationary phase is a very uniform
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Title of experiment 3: Gel Filtration Chromatography of LDH INTRODUCTION Gel filtration chromatography is a type of column chromatography in which separated protein‚ peptides and amino acids on their molecular size. The stationary phase consists of beads containing pores. The mobile phase is the solvent that is found both around the beads and in the pores of the stationary phase matrix. As the sample is passes through the column‚ the molecule that are larger than the pores will not retarded by
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Bangabandhu Sheikh Mujibur Rahman Science and Technology University‚ Gopalganj. Title : Assignment on HPLC (High-performance /High-Pressure Liquid Chromatography) Introduction: HPLC is an advanced form of liquid chromatography used in separating the complex mixture of molecules encountered in chemical and biological systems‚ in order to recognize better the role of individual molecules by using significantly higher pressure (50–350 bar). Compounds are separated by injecting a sample
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Division of Environmental Epidemiology University Utrecht 05/06/2007 Biopharmaceutical Technology Europe Volume 21‚ Issue 10 Valencio Salema‚ Lalit Saxena‚ Priyabrata Pattnaik ‘Removing endotoxin from biopharmaceutical solutions’ 01/09/2009 ‘Ion Chromatography: A New Technique for Clinical Chemistry’ Courtney Anderson Vol. 22‚ No. 9 1976 Biochemistry Jeremy M. Berg‚ John L. Tymoczko‚ Lubert Stryer 2006 ‘Curtin’s industrial biotechnology research’ (http://www.theborneopost.com/2013/02/12/curtins-industrial-biotechnology-research/)
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Contents Title Page No. 1.0 Introduction 1 2.0 Sample Treatment 3 3.0 Extraction 4 4.1 Solid-Phase Extraction 4.0 Separation 6 5.2 High Performance Liquid Chromatography (HPLC) 5.3 Thin Layer Chromatography (TLC) 5.0 Detection and Identification 7 6.4 UV-Vis Spectrophotometry 6.5 Mass Spectrometry 6.6 Nuclear Magnetic Resonance Spectrometry 6.0 Health Benefits of Daidzein 18
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