Limited‚ Bangalore College supervisor: Dr: T.Yasodha‚ Head- Department of Biotechnology‚ Shri Andal Alagar College of Engineering‚ Mamandur‚ Tamilnadu‚ India Objective: Purifying microbe derived therapeutics from fermentation broth by various chromatography and analysis using RP HPLC techniques- specializations on lipstatin drug. Abstract: The main focus area of this project is to purify the microbe derived drug lipstatin by using various trials of chromatographic columns with different packing
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HPLC Theory: System Suitability Parameters High performance liquid chromatography is defined as a separation of mixtures of compounds due to differences in their distribution equilibrium between two phases‚ the stationary phase packed inside columns and the mobile phase‚ delivered through the columns by high pressure pumps. Components whose distribution into the stationary phase is higher‚ are retained longer‚ and get separated from those with lower distribution into the stationary phase. The
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1F90 Experiment #2 Aerobic and Anaerobic Metabolism Caleb Coolen 5407754 Lab Section 21 Experiment Performed: October 10‚ 2013 Lab Partners: John Elgersma‚ Sandra-Lynn Plourde & Taylor Spratt Abstract: Materials and Methods: Methods and materials used in this experiment can be found in the BIOL 1F90 Laboratory Manual (Martin‚ 2013)‚ Experiment # 1‚ pages 2-5. Results: Table 1: Culture Sample Chromatography Rf Values Sample of Spot Distance from Origin (mm) Solvent Front (mm) Rf
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Caffeine is a drug that is used to improve mental alertness by stimulating the central nervous system [1]. It is an ingredient found in a various amount of products sold on the market today‚ such as coffee‚ tea‚ chocolate‚ soft drinks‚ and energy drinks. Caffeine can be taken by mouth or intravenously (by IV) to help alleviate breathing problems in infants and can even be used in combination with some pain killers to treat migraines after epidural anesthesia. Moreover‚ it can be helpful in preventing
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CHAPTER 1 INTRODUCTION Power transformers are very important in the electrical power sector; they are among the expensive assets of power generation and distribution systems. . According to Piush Verma [16]‚ transformers are static electromagnetic devices consisting of two or more windings which are linked with a common magnetic field used for the distribution of electrical power from one voltage level to another. Some of the main parts of the transformer are; the windings‚ tank and insulation
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Experiment 5 Analysis of Plant Pigment Purpose: Chromatography is used for separation. In this case the separation of plant pigments. After placing the plant sample in the column the separation will be easy to see and the samples will be analyzed using VIS spectrophotometry. This is used to isolate and identify the different lipids causing the different pigmentations. Introduction: Lipids are chemically diverse substances found in cells. Lipids are water-insoluble‚ structural components
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fatty acids. • Spectroscopic Techniques: Structural determination of an Organic compound by using UV & Visible ‚ IR ‚ NMR ‚ MASS techniques. • Chromatographic Techniques: Paper‚ Column ‚ Gas-Liquid chromatography ‚ High performance liquid chromatography etc. Computational Skills: Operating Systems : Win XP‚ 2000‚ 98‚ Languages : C‚ C++‚ Packages : Ms-Office. Soft Skills: 1. Good communication
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Tamilnadu. E-Mail: velmpharm24@gmail.com A precise‚ accurate and rapid RP-HPLC method has been developed and validated for the simultaneous estimation of Tamsulosin Hydrochloride and Finasteride in tablet dosage form. Chromatography was carried on RP – 18e Hiber RT (250 × 4.6) column using Water:Methanol (30:70%) v/v as mobile phase at a flow rate 0.7 ml/min and the effluent was monitored at 225 nm. The retention times of Tamsulosin Hydrochloride and Finasteride were 5.4
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Methanolic Extract of Eggplant (Solanum melogena) Peel as a Dye Pigment in Differential Stains Azucena‚ Alexis G. An Official Entry to 2010 Regional Science Fair Cluster 2 Physical Science- Individual Negros Occidental National Science High School January 13‚ 2011 Ma. Melanie P. Romero Research IIB Adviser APPROVAL SHEET The research paper attached hereto‚ entitled‚ “Methanolic Extract of Eggplant (Solanum melogena) Peel as a Dye Pigment in Differential Stains”‚ prepared and submitted
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Discussion: In the first part of the experiment‚ chronoamperometry was used to determine the diffusion coefficient of ferricyanide. The chronoamperogram can be seen in Graph 1. The chronoamperogram shows three distinct regions. [1A] Region “a” is where the electrode potential is higher than the ferricyanide’s potential‚ so no reduction reaction takes place. Region “b” is where the potential is decreased to a potential much lower than that of ferricyanide. This results in the ferricyanide consuming
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