Biological Sciences College of Arts and Sciences Visayas State Unversity Visca‚ Babay City‚ Leyte Name: Millama‚ Engene O. Course & Year: BS Biotech-2 Lab Schedule: W (1-4) Group 5 Date Performed: Dec. 12‚ 2012 Date Submitted: Jan. 9‚ 2013 Separation of Photosynthetic Pigment by Paper Chromatography I. INTRODUCTION There are different types of components in plant pigments. The most important and abundant chemical pigment found in plants is chlorophyll. This pigment
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...................................................................... 12 4. Basic principles and applications of Potentiometry .................. 16 II. Separation techniques ............................................................ 25 1. Chromatography ................................................................... 25 2. Electrophoresis ..................................................................... 53 III. Immunoassays .............................................................
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SINGAPORE Chemical Engineering Process Laboratory II Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification
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The Chromatography of Food Dyes and determination Of the Dyes Present in M&M Candies Procedure To complete this lab‚ I first mixed the solvent solution consisting of the proper ratio of water‚ salt‚ and isopropyl alcohol in a Pyrex measuring cup. I then prepared my chromatography papers for 2 trials by drawing‚ with a pencil‚ the appropriate lines and labels. Using a toothpick‚ I added the color to the paper‚ and then repeated until the color was strong. I repeated this step several more
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experiment was for each student to use column chromatography to separate plant pigments from spinach leaves. Some goals and objectives were to observe the effect of the increasing polar eluent fractions‚ to analyze the different fractions collected‚ and to correlate the structure of the components with polarity and behavior on a chromatographic column. Background The best method for separating mixtures in organic chemistry is chromatography. Chromatography is the separated of a mixture of two or more
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ENVIRONMENTAL TECHNOLOGY DEPARTMENT Lab Report ROOM NO: FE E309 EXPERIMENT NO : 8 TITLE : Thin Layer Chromatography of Food Dyes Submitted by Class Partners Instructor : Lyndsay Grover : BIOL 10000 lab. : Awatif Hagelamin : Farag Soliman Date lab performed : February 3‚ 2011 Date of submission : February 10‚ 2011 FENNELL CAMPUS HAMILTON‚ ONTARIO 1/3 Purpose: The purpose of this experiment was to observe the reactions of food dyes with thin layer chromatography paper in order to find the
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Alkenes from Alcohols: Analysis of a Mixture by Gas Chromatography Aim: To analyze a mixture of alkenes by gas chromatography. Introduction: In this lab‚ we specifically used elimination reaction; however we only used the E1 reaction. In the presence of strong acids‚ alcohols protonate to form a good leaving group‚ namely water. Upon loss of a proton to a good leaving group‚ an introduction of unsaturation (a double bond) can be preformed. According to Wikipedia‚ an E2 reaction is typically
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Title: Candy Chromatography Background Information and Research: Paper Chromatogrpahy is a analytical method that is used for seperating and identifying mixtures of color. There are two stages‚ the stationary phase and the mobile phase. The first is for a solid and the second for a liquid or gas. -Reference http://www.chemguide.co.uk/analysis/chromatography/paper.html Purpose: To see how paper chromatography works. Materials: · Candy with a colored coating‚ like Skittles® or M&Ms® (4 different
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Gas chromatography is the common method used for the biogasoline analysis. Biogasoline catalytic cracking process requires detailed qualitative and quantitative analysis of the hydrocarbon composition of its oil and fraction. In this analysis‚ their fractional composition‚ saturated vapor pressure‚ density and antiknock rating will be investigated. By knowing the qualitative and quantitative composition of the gasolines‚ the content of n-paraffin‚ isoparaffin‚ aromatic‚ naphthene‚ and olefin (PIANO)
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Thin Layer Chromatography of the Unknown Analgesic Jessica Bajao*‚ Phoebe Abalos‚ Kevin Antiga‚ Carmelus Aseneta 3-Biology 2 College of Science‚ University of Santo Tomas‚ Manila‚ Philippines Abstract The group used five different analgesics in this experiment: aspirin‚ acetaminophen‚ ibuprofen‚ caffeine‚ and mefenamic acid. Six analgesics were spotted on the TLC plate including the unknown. After the development of the TLC plate‚ it was placed under ultraviolet light for the spots resulted
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