and centrifuged for 20 seconds. A polyacrylamide gel was assembled and the samples were loaded into it. The gel ran for approximately 45 minutes at 160 V. A western blot analysis was done in order to examine the Erk1/2 phosphorylation of the NIH3T3 cells. Three pieces of Whatman paper were wet in transfer buffer and placed on the anode plate. A wet PVDF membrane was placed on top of the Whatman paper. The gel was removed from holder and the stacking gel was removed. Forceps were used to place the gel
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Determining the Concentration of an Unknown Solution Graph: The effect of concentration of CuS04 * 5H20 on transmittance. I solved for my unknown by plugging in “y” as my given transmittance value‚ which was 85.0. y = -278x + 100.12 .85 = -278x + 100.12 -99.27 = -278x X = .357M (concentration) The relationship between the transmittance values and the concentration is an indirect relationship. Whenever the concentration increases‚ the transmittance decreases. It can also be reversed
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Pre-Laboratory Questions 1. Yeast cells look like separate little spheres; they cannot walk or swim. Brainstorm methods by which yeast cells might communicate with each other. Record your list of possibilities in the space below. I. Signal Cascade II. Hormones III. Pheromones IV. Receptors embedded in the cell V. Growth near another cell Guided Activity: Table Charts Alpha – Type Culture Single Haploid Cells Budding Haploid cells Time 0 Field 1 3 3 Field 2 1 1 Field 3 8 2
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Conductivity of electrolyte solutions (Dated: October 29‚ 2009) I. INTRODUCTION Pure water does not conduct electricity‚ but any solvated ionic species would contribute to conduction of electricity. An ionically conducting solution is called an electrolyte solution and the compound‚ which produces the ions as it dissolves‚ is called an electrolyte. A strong electrolyte is a compound that will completely dissociate into ions in water. Correspondingly‚ a weak electrolyte dissolves only partially
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BioLab3 Cell Membranes Lab Report Student Name: I. Diffusion Define the following terms. Solvent Solute Solution. Diffusion Concentration gradient Dynamic equilibrium EXERCISE 1 – Factors influencing rate of diffusion Predict how molecule size and temperature will affect the outcome of this experiment. Record the data from the information in the lab. Potassium Permanganate R.T. Methylene Blue R.T. Time (min) Total Diameter
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Chou‚ P. (2007). The effects of background music on the reading performance of taiwanese esl students. New York: Self Published. Retrieved from http://books.google.com/books?id=zVz_Lv9OnN0C&pg=PA33&lpg=PA33&dq=effects of background music on concentration&source=bl&ots=xRZ4pvia4r&sig=lzJhKQmZfw7WXV-WD1hyJhT9I4A&hl=en&sa=X&ei=I3otUeKHBo_o8gSBooHYAw&ved=0CH8Q6AEwCQ * Hallman‚ Price‚ Katsarou‚ S. J. G. (2002‚ November 2).The effects of background music on
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have made a difference in the results. The mood of the person experimenting could possibly alter the results. For example‚ if a person is under stress‚ they may accidentally squeeze too much altering the rate of diffusion. If the water was warmer‚ this may have sped the diffusion process because the molecules would have been moving faster. If the water was freezing‚ this may have slowed the diffusion process because the molecules may have been moving slower. Part B: Title: The effect of salt concentration
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Concentration of liquid foods is a fundamental operation in many food processes; it is completely different from dehydration. Usually‚ foods‚ which are concentrated‚ remain in the liquid state; while drying produces solid or semisolid foods with significantly lower water content. The concentration of liquid foods has three different methods; evaporation‚ membrane concentration‚ and freeze concentration. Evaporation usages gas liquid phase separation. It has the lowest capital cost and the maximum
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Determining the Concentration of a Solution: Beer’s Law Purpose: The purpose of the experiment is to determine the concentration and formula of an unknown cobalt nitrate solution by measuring absorbance. Introduction: A Colorimeter will be used to determine the concentration and formula of an unknown cobalt nitrate solution. The colorimeter sends blue light from the LED light source to pass through the solution and hit a photocell. A solution with a higher concentration will absorb more
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Comparing Plant and Animal Cells Hypothesis: I predict I will see the following organelles when observing a plant and animal cell under a microscope: the cell membrane‚ cell wall‚ nucleus‚ and the endoplasmic reticulum. Frog Blood: Analysis: 1. The shape of the frog blood cell was in a circle‚ or oval. The edges were a little irregular‚ but it could still be identified as‚ basically‚ a circular shape. 2. The cells were produced by an animal‚ a frog. 3. The cell wall was not present
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