The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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Conclusion I predicted that if the decolorization of DPIP is caused by photosynthesis and not cell respiration and spinach extract containing chloroplasts and mitochondria is incubated with DPIP‚ then the rate of DPIP decolorization should be higher if in bright light verses dark light because DPIP is reduced by photosynthesis and not by the mitochondria or any other cellular function. If DPIP was only decolorized by chloroplasts‚ then the percent transmittance of chloroplast suspensions would be
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Introduction The purpose of this lab is to determine which pigments in a plant support or effect photosynthesis‚ based on starch production‚ which wavelengths of light are involved in photosynthesis‚ and identify plant pigments found in a plant leaf by means of paper chromatography. Life on Earth is dependent entirely on the energy from the Sun‚ not only to keep the planet at a suitable temperature but also to provide the energy required to sustain life. The energy of the Sun‚ in the form of
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Virtual Lab: Optimizing Photosynthesis Maria Diaz March 12‚2012 Pre-Lab Questions 1. Which two variables are controlled in a similar manner? The two variables that can be controlled in a similar manner are CO2 & the light level. 2. How can you get the results to the investigation faster? You can get the results to the investigation faster by adjusting the speed at the bottom of the water weed simulation. 3. How will you be able to determine the rate of photosynthesis
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Introduction The lung is a spongy organ made up of of irregularly shaped air spaces called Alveoli. The alveoli are lined by a single layer of flat cells and supported by a mesh of fine elastic fibers. The alveoli are surrounded by a rich network of pulmonary capillaries (Couch and Berger‚ 2004). Our lungs are a network of connected tubes that bring oxygen from the air into our blood‚ nourishing the trillions of cells that make up our bodies. The lungs also clean the blood of carbon dioxide waste
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Photosynthesis Lab Gizmo website: www.explorelearning.com Class code: EFVNHTVRGY Gizmo Warm-up During photosynthesis‚ plants use the energy of light to produce glucose (C6H12O6) from carbon dioxide (CO2)‚ and water (H2O). Glucose is a simple sugar that plants use for energy and as a building block for larger molecules. A by-product of photosynthesis is oxygen. Plants use some of the oxygen they produce‚ but most of it is released. In the Photosynthesis Lab Gizmo™‚ you
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Title: Rate of Photosynthesis Introduction: Photosynthesis occurs in plants where the light energy changes to chemical energy. This process occurs in the chloroplast of the plant cell in order to have gas exchange in the organism. Purpose: To measure the effect of light intensity on the rate of photosynthesis in the leaf cells. Hypothesis: The higher the concentration of the sodium bicarbonate solution‚ the faster the leaf of the plant will rise to the surface of the water in the beaker‚ the
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_______(savanna chatham)_________________ Virtual Lab #5- “Which Colors of the Light Spectrum are Most Important for Plant Growth? Instructions: Open the link to Launch “Virtual Lab #6- Photosynthesis” Read all instructions on the left-hand side of the lab. Watch the short Video at the bottom of the lab Complete the “Pre-Lab” Questions. Complete the Lab Activity Record your findings in the “Results” and “Post-Lab” sections and answer all review questions Pre-Lab: 1.) List all of the colors found in the visible
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BIOLOGY DCN PHOTOSYNTHESIS LAB # 6 Effect of varying coloured filters on the photosynthetic rate of spinach chloroplasts The data below is just representative of what trends and relationships you were supposed to see. Numbers can vary. Absorbance at 620 nm for each treatment DCPIP + chloroplasts t=0 min. 0.93 0.945 0.905 0.915 t=3 min. 0.95 0.731 0.83 0.816 change in A620 -0.02 0.214 0.075 0.099 t=0 min. t=3 min. change in A620 t=0 min. t=3 min. change
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Through this experiment‚ Egeria densa was observed using a microscope. The task was to observe and identify the different types of cell‚ cytoplasmic streaming‚ and plasmolysis of Egeria densa. First‚ the microscope was examined and investigated to master the use of the equipment. A microscope slide grid which was on the slide glass was required to be seen clearly using 4x‚ 10x and 30x. During the latter part of the experiment‚ the Egeria densa was observed using the microscope to understand the
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