Organic Chemistry 12A Professor Alston October 28‚ 2014 Isolation of Chlorophyll and Carotenoid Pigments from Spinach Purpose The purpose of this experiment was to isolate ß-carotene‚ chlorophyll-A‚ and chlorophyll-B from spinach using column chromatography. Spinach was dehydrated using ethanol‚ and the pigments were extracted with dichloromethane. The spinach extracts were dried using CaCl2. Then‚ the solid pigments were run through a column using a non-polar solvent‚ hexane. The polar absorbent
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of Chemical Engineering CHME 426 –Chemical Engineering Laboratory III Title Page (Full Report) Title of the Experiment: Reaction through three CSTR in series Submitted by: Group (4) Section: Female 1. Name: Amina Ali ID: 200550284 2. Name: Duaa Tabarak ID: 200553858 3. Name: Mariam Rustom ID: 200552242 Date of experiment: 31st March‚ 2010 Date of submission: 11th April‚ 2010 Grades: Report presentation………………………………………… /15 Abstract and Objective(s)……………………………………/10 Introduction
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total of 15 points. To receive full credit for Exercise 8‚ you must submit the following: 1) The typed Exercise 8 report form‚ complete with all of your data recorded and questions answered. 2) A full scientific report for the Part I experiment that your group conducted. Use the supplemental guide to writing a scientific report to help you produce this report. Part 1: Does light matter? Table 8.1: Group and Class rates for Photosynthesis by Elodea sprigs. |Light Condition
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The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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Separating Substances: Identifying Food Dyes with TLC Background The color of food is an integral part of our culture and enjoyment of life. Who would deny the mouth-watering appeal of a deep-pink strawberry ice cream on a hot summer’s day or a golden Thanksgiving turkey garnished with fresh green parsley? Even early civilizations such as the Romans recognized that people "eat with their eyes" as well as their palates. Saffron and other spices were often used to provide a rich yellow color
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:___________ Chemistry Laboratory 101__ Date Submitted[1] :___________ Members[2]: Instructor’s Initials[3] :___________ 1. _____________________ 2. _____________________ 3. _____________________ 4. _____________________ Laboratory Report Sheet The Bunsen Burner Activity 1 Objectives:4 1. ________________________________________________________ 2. ________________________________________________________ 3. ________________________________________________________
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TITLE OF EXPERIMENT 2 : DILUTION 2.1: OBJECTIVES Upon completion of this experiment‚ students should be able to: 1. determine the concentration of coloured solution such as FeCl3 by using dilution and colour differentiating (colorimetric) techniques. 2.2: INTRODUCTION Concentration can be expressed in many different ways such as percentage volume and percentage weight etc. In laboratory‚ normally concentrations were expressed as molarity and normality. Molarity is the
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[pic] Department of Chemical & Biomolecular Engineering THE NATIONAL UNIVERSITY Of SINGAPORE Chemical Engineering Process Laboratory I SEMESTER 4 Experiment F2 Flow Measurement in Closed Conduit and Centrifugal Pump Characteristics Name : Ang Sok Gek Chai Chang Er Cherry Chen Mingli Matriculation No. : U046941L U046938W U046882J Group : Th1 Date of Experiment : 16th March 2006 Table of Contents
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References: http://webs.mn.catholic.edu.au/physics/emery/measurement.htm#Measurement http://www.digipac.ca/chemical/sigfigs/experimental_errors.htm http://www.tsb.gc.ca/eng/rapports-reports/rail/2011/r11v0057/r11v0057.pdf
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EXPERIMENT NO. 1 INTRODUCTION TO LAB INSTRUMENTS. 1. BREADBOARD We should be familiar to the following things about a breadboard: * What is a breadboard and what is it used for? * How does it work? * Setting Up. * Limitations. What is a breadboard and what is it used for? A breadboard (or protoboard) is usually a construction base for prototyping of electronics. The term "breadboard" is commonly used to refer to a solderless breadboard (plugboard). It was designed by
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