GRAM STAINING EXPERIMENT CONDUCTED ON 9/29/2013 Introduction: The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. This gram stain technique was discovered by Hans Christina Gram in 1884. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive
Premium Gram staining Bacteria Staining
The purpose of this experiment was to study the effect acid rain has on seed germination and after performing this experiment is can be concluded that acid rain has a negative effect on seed germination. When seeds are soaked in an acidic solution the process of germination slows. Acidic solutions‚ while do slow growth of a seed‚ they do not kill the seed. A seed can be dormant for a majority of its lifespan‚ seeds watered with acid solutions stayed in a dormant condition. For example‚ trial three
Premium
Exercise 10: Acid-Base Balance: Activity 2: Rebreathing Lab Report Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. In cases of acidosis‚ the pH of the blood is You correctly answered: c. less than 7.35. 2. Carbon dioxide and water form You correctly answered: a. carbonic acid (a weak acid). 3. Which of the following is true of respiratory acidosis? You correctly answered: c. The amount of carbon dioxide in the blood is greater than normal. 4. Rebreathing You correctly
Premium Carbon dioxide Bicarbonate Blood
Microbiology Laboratory Report Identification of Unknown Bacteria 03/10/05- 04/01/05 Authors: Richard Hendricks‚ Jessica Prebish; NMU Abstract: Broth culture 16 was randomly selected by our group and subjected to qualitative tests for taxonomic identification. The culture did appear homogenous throughout the testing period and is currently retained by Northern Michigan University’s department of Microbiology. We suggest that culture 16 is an example of Escherichia coli. Background: Techniques
Premium Escherichia coli Staining Gram staining
proficiency and should be able to: A. describe staining; B. enumerate the common stains; C. differentiate between an acidic dye and a basic dye; D. compare simple‚ differential and special stains; and E. list the steps in preparing a Gram stain and describe the appearance of a gram-positive and gram- negative cells after each step.\ II. SUBJECT MATTER: Biological Techniques Topic: Staining A. Materials: * cartolina- used during
Premium Staining Gram staining
Gram Staining of Bacteria Since bacteria is really small microbiologist use stains to help them see bacteria more clearly under the microscope. Many of the stains they use color the bacteria cells directly and are called direct stains. Bacteria cells have a slightly negative charge while direct stains have a slightly positive charge that helps the stain bin to the bacteria. The strength of the binding from the stains depends on the make-up of the cell wall itself. Gram staining is the most helpful
Premium Staining Bacteria Gram staining
Identification of Unknown # 15 Abstract. One of the most fundamental differential staining techniques used in the study of bacteriology is gram staining. There are two main types of bacteria‚ gram negative and gram-positive. The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number 15. The following are the tests that were used to identify the two different bacteria. The
Premium Bacteria Microbiology Gram staining
Name ______________________________________ Before you begin‚ save this Lab Report Template on your computer as LastNameAPChem7 Title: Acid Base Titration Purpose: To practice titration techniques To perform acid-base titrations To determine the molarity and percent composition of acetic acid in commercial vinegar Materials: |0.1 M sodium hydroxide |24-well reaction plate |toothpicks | |phenolphthalein test paper
Premium Sodium hydroxide Base PH indicator
Gram Staining Gram Staining is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). Gram Staining is a way to separate one large group of bacteria into two. Crystal violet is used to dye the cells‚ which is the primary stain. Those that retain the color are grouped as Gram-positive‚ and those that do not retain the color are grouped as Gram-negative. Many of the Gram-negative bacteria are pathogenic‚ making this process useful for detecting infections
Premium Gram staining Bacteria Staining
The Gram staining method is named after the Danish bacteriologist who originally developed it in 1882‚ Hans Christian Gram. The Gram staining process is one of the most important staining techniques in microbiology. It is almost always the first test performed for the identification of bacteria. The primary stain of the Gram’s method is either crystal violet or methylene blue‚ each almost equally effective. The microorganisms that retain the crystal violet and iodine mixture appear purplish brown
Premium Gram staining Staining Microbiology