EXPERIMENT 5: CHROMATOGRAPHY Abstract Paper Chromatography uses a chromatogram paper as its stationary phase and the solvent as the mobile phase. Retention factor is the ratio of the distance travelled by the sample to the distance travelled by the solvent. This experiment aims to separate organic compounds‚ to compute Rf values and to identify unknown compounds using Rf values. The ten samples underwent paper chromatography to determine the components of the unknown sample. The mobile phase allowed
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Chromatography is a process used to separate mixtures. The word chromatography is derived from the Greek words ‘khroma’ and ‘graphein’ meaning ‘color’ and ‘to write’ or to represent. Although there are a couple different types of chromatography‚ in each case a substance is placed onto or into a medium and a solvent is passed through the test substance. In chromatography science‚ the solvent is called the mobile phase or the carrier fluid and the medium is called the stationary phase. There are four
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How Chromatography Helps The Human Race Chromatography is the separation of a mixture by passing it in solution or suspension or as a vapor (as in gas chromatography) through a medium in which the components move at different rates. “Chromatography is done by making of a mixture move past the solids‚ or across the surface of a solid‚ like paper. The mixture is poured onto a solid surface. As the different components of the liquid run down the solid‚ some of them move more slowly than other. A component
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Candy chromatography is a method of analysis of separating and comparing of dyes used in candies and food coloring. It is also a proven method to analyze candy and its complex mixture‚ without having to taste it. These complex mixtures can be separated back into pure substances. To find out the ingredients of a favorite candy‚ chromatography would help to define every ingredient found in the candy sample. The word chromatography is derived from the two Greek words‚ "chroma" meaning color
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Visual Arts Topic: Textile and Design Manipulation Topic: Tie Dye Time: 4 sessions x 30 mins each General Objective To be able to use appropriate materials to create designs on fabric by various tie dyeing techniques. Specific Objectives At the end of the lesson students should be able to: ✓ 1. Define Tie Dye ✓ 2. List materials needed for tie dyeing ✓ 3. Explain Tie Dye as a resist dyeing ✓ 4. Know different techniques and explain each of
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answer through your investigations: 1. Which fabric (a) cotton‚ a natural fiber and (b) rayon‚ a synthetic fiber is best dyed with mayana-alum dye combination? 2. Often‚ mordants are added to fix the dye onto the fabrics. These are usually inorganic oxides or salts which form colored complex with the dye. Which mordant is more suited for dyeing cotton with dye extracted from eggplant skins? 3. Which mordant is more suitable for dyeing a synthetic fiber with the synthetic extract from mangosteen
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Natural dyes are dyes or colorants derived from plants‚ invertebrates‚a or minerals. The majority of natural dyes are vegetable dyes from plant sources – roots‚ berries‚ bark‚ leaves‚ and wood — and other organic sources such as fungi and lichens. Archaeologists have found evidence of textile dyeing dating back to the Neolithic period. In China‚ dyeing with plants‚ barks and insects has been traced back more than 5‚000 years.[1] The essential process of dyeing changed little over time. Typically
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Paper Chromatography Chromatography comes from the Greek word khromatos (color) and graphein (to write); i.e. paper chromatography is literally color writing on paper. This method tests the purity of compounds and identifies substances. This analytical process‚ despite having been replaced by the success of thin layer chromatography‚ still stands as a valuable teaching tool‚ and is nevertheless very common. This method is very useful because not only is it a relatively quick process‚ but also
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Chromatography Abstract Paper chromatography is one of the methods under chromatography‚ it can use in identifying unknown compounds using known compound and it can also use as a separation technique based on the differences in affinities of components of the mixture to a stationary phase and a mobile phase. In the experiment‚ the stationary phase was the filter paper onto which the dye samples were dropped onto while the mobile phase was the solvent mixture containing ethanol and water which
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LAB REPORT NUMBER TWO DATE: 3/25/2010 inal attachment Lab Experiment number 11 PURPOSE: To learn the Gram stain technique‚ the reason for the stain‚ and how to identify the results of the organisms stained. MATERIALS: Bunsen burner‚ inoculating loop‚ staining tray‚ glass slides‚ bibulous paper‚ lens paper‚ oil‚ and microscope METHODS: Apply Crystal Violet (Primary stain) for 1 minute. Rinse with D-water Apply Iodine (Mordant) for 1 minute. Rinse with D-water. Apply Alcohol (Decolorize) for
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