by Laboratory Reagents‚ Legacy Product Code: S/0380/48‚ CAS Number:8047-15-2) were powdered and analyzed as potassium bromide (KBr) pellets using FTIR (Model-JASCO FT/IR 4100 LE‚ made in Japan; Range: 4000-400 cm-1) Prabhu et al. (2013). Gas Chromatography–Mass Spectrometry (GC–MS) analysis: Acid hydrolysis of saponin was carried out according to the method described by Metwally et al. (2012) with some modification. Saponin was hydrolyzed with 2 N HCL (6 hr at 100 o C) under reflux‚ the residue
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5.05 Mixtures and solutions Lab report Ink Chromatography Chromatography is also used to compare and describe chemical substances. The chromatographic sequence of sorbed substances is related to their atomic and molecular structures. A change in a chemical substance produced by a chemical or biological reaction often alters the solubility and migration rate. With this knowledge‚ alterations or changes can be detected in the substance. Chromatography serves mainly as a tool for the examination and
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Chromatography: How can we separate a mixture? Purpose The chromatography lab is to understand how molecules with similar molecular properties can be separated with paper chromatography. These differences will be interpreted to see the distinction of separate chemical substances. Pre Lab Questions 1. Explain capillary action as it pertains to water and paper. Capillary action makes water draw up the paper. As paper absorbs water mixes with the solutions in the paper. 2. What is the
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TA: Gia Hoang Lab 1: Effect of pH on a Food Preservative 9-1-2011 Purpose The purpose of this lab was to determine if a new substance forms when sodium benzoate is placed in a simulated stomach acid. Theory Benzoic acid can be found in a wide variety of plants. It is used as a natural preservative in many foods. Sodium benzoate‚ the salt of benzoic acid‚ has become a more popular choice as a food preservative because it is more soluble in water than benzoic acid. Many food items found today
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Information and Research 1. Chromatography is an analytical methor or technique that serves mainly as a tool for the examination‚ separating and identifying mixtures of chemical substances that are or can be coloured. 2.check the presence of any contamination in the manufactured compounds h as medicine‚ Contaminants in rainwater Analysis of narcotics Detection of substances in urine http://en.wikipedia.org/wiki/Paper_chromatography http://www.chemguide.co.uk/analysis/chromatography/paper.html http://chem
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Decolorization of Crystal violet dye by Aeromonas hydrophila isolated from textile wastewater Sujata Mani and Ram Naresh Bharagava* Laboratory of Bioremediation and Metagenomics Research (LBMR) Department of Environmental Microbiology (DEM)‚ School for Environmental Sciences (SES) Babasaheb Bhimrao Ambedkar University (A Central University) Vidya Vihar‚ Raebareli Road‚ Lucknow - 226 025 (U.P.)‚ India *Corresponding Author: Dr. R. N. Bharagava Tel.: (+91) 522-2998718; Fax: (+91)
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Purpose In this lab‚ we used PCR and gel electrophoresis to identify genetically modified food. Introduction A genetically modified organism is an organism whose DNA or genetic makeup has been modified to code for certain desirable traits("Genetically Modified Foods"). Common genetically modified plants include corn and soy‚ and common genetically modified animals are fish. Many genetically modified plants are coded to resist bugs‚ grow faster‚ and produce bigger fruit‚ while most GMO animals
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Fermented foods are made by the partial oxidation of sugars‚ or sometimes other organic molecules‚ in a food product. The difference between this and the normal cellular respiration is that the electron donor from the electron transport chain is an organic compound rather than oxygen. One challenge for producing fermented foods is the pH levels of the food. Bacteria have different optimal pH levels. Therefore‚ as pH changes due to fermentation different bacteria will begin to ferment the food. For example
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Abstract: The purpose of this lab experiment was to discover genetically modified organisms. We analyzed our results and found that our sample was GMO positive. These results were attained through polymerase chain reaction and agarose gel electrophoresis. We came to the conclusion that the presence of a band in lane 4 confirms that there are indeed genetically modified organisms. Introduction: Overall‚ the main purpose of this experiment was to conduct gel electrophoresis combined with PCR in
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CHEM 2204 Chromatography Lab by wyk.wong » Fri Jul 11‚ 2014 10:25 am Results and Calculations Rf values Rf=(Distance moved by the spot (cm))/(Distance moved by the solvent front (cm)) Toluene: Rf=2 cm/3.8 cm=0.53 (Fluorenone) Rf=1.1 cm/3.8 cm=0.29 (Fluorene) Hexane: Rf=1.8 cm/2.2 cm=0.82 (Fluorene) Rf=0 cm/2.2 cm=0 (Fluorene Table 1: Experimental IR peaks compared to literature IR peaks for fluorenone Functional group Experimental peak (cm-1) Literature peak (cm-1) C-H 3010.5 3013
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