ENZYMES LABORATORY REPORT Introduction The utilization of any complex molecule for energy by an organism is dependent on a process called hydrolysis. Hydrolysis breaks complex molecules into simpler molecules using water. Similarly‚ the process that is the reverse of this is called dehydration synthesis‚ which removes water from simpler molecules. However‚ because hydrolysis occurs very slowly‚ living organisms use biochemical’s called enzymes to speed up the reaction. In this lab exercise
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Investigation on the effects of Temperature‚ pH levels‚ and Enzyme Concentration on the reaction rate of the Decomposition of Hydrogen Peroxide by Catalase The purpose of this investigation is to figure out how temperature‚ pH‚ and enzyme concentration affect the reaction rate of an enzyme. It’s important to understand how certain factors affect enzymes because of their crucial role in the metabolic processes of life. Enzymes lower the activation energy necessary for a chemical reaction to
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To begin the analysis‚ the properties of the air at the base of the tower were examined. The wet and dry bulb temperatures of the air at the inlet were used to find the humidity‚ humid volume‚ and enthalpy of dry air. For example‚ in the first trial the dry bulb temperature was 22.5⁰C and the wet bulb was 16.5⁰C‚ and the properties found from the psychrometric chart are listed below. H_bottom=0.0095 kg/(kg dry air) V_bottom=0.85 m^3/(kg dry air) h_bottom=47 kg/(kg dry air) Then‚ the cross-sectional
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most notable variables is the temperature of the plant’s environment. Previous study has led me to conclude that when the temperature of the plants’ environment is low‚ the rate of photosynthesis is also low because the enzymes responsible for photosynthesis are not able to absorb as much heat energy and in turn will move slower. In contrast‚ if the temperature of the plant’s environment increases‚ the rate of photosynthesis will also increase because the enzymes and molecules responsible for
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Ah Seung Chong Molecular Biology CTW: Enzyme Kinetic Dr. Cruz 07/22/2010 Enzyme kinetics Introduction Enzymes are biological catalysts or assistants‚ without enzyme many of important processes of life could not happen. Most of enzymes are proteins that help speed up chemical reactions by lowering amount of activation energy needed for the reaction1. Enzymes are usually highly selective‚ only bind to specific substrate and convert it to product at a particular rate1. The rate of the reaction
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8/12/2013 Intro I am going to investigate a factor that affects the rate of enzyme activity What are enzymes and how do they work? Enzymes are biological catalysts - catalysts are substances that increase the rate of chemical reactions without being used up. Enzymes are also proteins and thus have a specific shape. They are therefore specific in the reactions that they catalyse - one enzyme will react with molecules of one substrate. The site of the reaction occurs in an area on
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ID:0720052 Effect of an Increasing Substrate Concentration on Enzyme Activity Rate Abstract The reaction rate of an enzyme can be affected by many factors‚ and the purpose of this experiment was to find out how an increasing substrate concentration influences the rate of an enzyme activity; we obtained data from recording the absorbance of the samples which contain the same amount of potato juice (enzyme oxidase) and different amount of catechol (substrate) while holding pH and temperature constant
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For this experiment‚ we started off by taking tubes numbered 1-4 and started adding one scoop of our enzyme catalyst‚ in this case‚ the yeast. We then proceeded to measure and add 1 mL of distilled water to test tubes A-D. To get a more accurate measure of 1 mL of distilled water‚ we used the dropper labeled “W” to drop distilled water into the 5 mL graduated cylinder until we saw that the bottom of the water line reached closely to 1 mL. Next‚ we took the four tubes with the scoop of yeast and
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Enzymes are catalysts that speed up chemical reaction but are not themselves consumed or changed by the reaction. The cell’s biological catalysts are proteins. Proteins are made up of one or more polypeptide chains that are folded to make an active site‚ an area in which a material to be acted on by the enzyme‚ called the substrate‚ will fit. The temperature‚pH‚ the concentration of enzyme‚ and the concentration of substrate all affect the activity of the enzyme and the rate of the reaction. The
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Rate of Enzyme Activity Justin Hunter G. Kim September 16‚ 2011 September 26‚ 2011 Abstract Catalase is a common enzyme found in nearly all living organisms that is frequently used by cells to rapidly catalyze the decomposition of hydrogen peroxide into less reactive oxygen and water molecules. Catalase is a protein that is most commonly found in the liver. The purpose of this experiment was to determine the effect that changes in temperature and pH have on the function of the enzyme catalase
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