Catalase Experiment Research Question: How does the weight of a piece of liver (0.4g‚ 0.8g‚ 1.2g and 1.6g in this case) affect the amount of gas created after 2 minutes when combined with hydrogen peroxide? Aim: To see find out how the amount of catalase correlates with the amount of gas created when in combination with hydrogen peroxide. Materials: 15g of liver 25 ml of hydrogen peroxide 1 scale 1 bucket 1 100ml graduated cylinder 1 250ml flask with bung 1 50cm tube (able to fix onto top of flask)
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tablets. In the experiment they first tested how long an Alka-Seltzer tablet would dissolve in cold water ( 9 degrees) then repeated it two more times in order to calculate a mean. Then using a hot plate to heat the water‚ they tested another tablet in the hot water (51 degrees) to see how long it would take to dissolve and again did it two more times to calculate a mean. Finally they tested another tablet in water left on the hot plate for thirty-seconds (81 degrees)‚ preformed the experiment for two more
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For this experiment‚ we started off by taking tubes numbered 1-4 and started adding one scoop of our enzyme catalyst‚ in this case‚ the yeast. We then proceeded to measure and add 1 mL of distilled water to test tubes A-D. To get a more accurate measure of 1 mL of distilled water‚ we used the dropper labeled “W” to drop distilled water into the 5 mL graduated cylinder until we saw that the bottom of the water line reached closely to 1 mL. Next‚ we took the four tubes with the scoop of yeast and
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affects the activity of amylase enzyme and the optimal temperature for fungal and bacterial amylase‚ the activity of these enzymes were tested at different temperature and times and iodine was used as indicator of presence of starch. During the experiment we observed that bacterial amylase had faster enzymatic activity than fungal amylase; bacterial amylase achieved 100% hydrolysis at 40°c and 60°c at 2 minutes time and fungal amylase achieved partial hydrolysis at 60°c at 4 minutes time. With
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Abstract: This experiment will determine if jumping beans are alive. We tested two groups. Group A and B. We put them in two different areas. Three beans were put in a lit area and three were put in a shaded area. It was found that if the depending on how lit the area is the more movement we saw from the jumping bean. Therefore it can be said that jumping beans are alive and respond to stimuli. Introduction: This Experiment “ Mexican Jumping Beans” focuses on how jumping beans respond to different
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In Graph 1‚ each line of color represents a different level of pH added to the solution. When pH 9 was added‚ it produced the highest rate of reaction (the most oxygen was produced)‚ whereas when the more acidic pH 5 was added‚ the rate of reaction was much slower‚ and less efficient. The higher the oxygen evolved (gas produced)‚ the more reactions were being produced/higher rate of reaction. Different types of enzymes’ reaction proportions differ based on the pH being added. For example‚ intestinal
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mixed culture tube by running a series of experiments to detect which specific Gram negative organism we had. To detect your gram positive from the mixed culture was given as extra credit points also. A Gram stain was performed and isolation streak plate in order to isolate and observe the unknown organism. Before the series of test‚ a dichotomous key had to be written up in order to know what steps and tests to run to identify the unknown Gram negative organism. I had to run three experiments in all
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Lab Report - Microbes Aim: To investigate four areas of the school and to find out which of the four have the most microbes. Areas to Sample: 1. Girl’s locker room (Senior school) 2. Girl’s locker room (Elementary school) 3. Boy’s locker room (Senior school) 4. Boy’s locker room (Elementary school) Hypothesis: We predict that the boy’s locker room in the senior school will have the most microbes. First of all‚ there are more people using our locker rooms in the Senior School
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Abstract:Conjugation is a natural occurring process that involves the transfer of DNA from one cell into another through a physical connection between the cells. In the following experiment‚ two strains of Escherichia coli bacterial cells (donor F’lac+strs and recipient F-lac-strr) underwent conjugation to produce a transconjugant strain (F’lac+strr). MAC plates and streptomycin were utilized to determine if conjugation had occurred. When plated‚ the donor colonies appeared red and the recipient
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Treatment of Results 1) What is the role of 0.25M sucrose as the medium for the fractionation process? Cold sucrose does not chemically react with cell organelles Due to the density and size of sucrose molecules‚ it is able to suspend pellets for configuration while providing a solution where the centrifugation can be better balanced Sucrose offers a liquid medium in which less dense fractions can be poured off as supernatant at the end of each centrifugation step. 0.25M sucrose solution
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