B. enumerate the common stains; C. differentiate between an acidic dye and a basic dye; D. compare simple‚ differential and special stains; and E. list the steps in preparing a Gram stain and describe the appearance of a gram-positive and gram- negative cells after each step.\ II. SUBJECT MATTER: Biological Techniques Topic: Staining A. Materials: * cartolina- used during the activity in lesson proper(flashcards) * iodine- used
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One of the most fundamental differential staining techniques used in the study of bacteriology is gram staining. There are two main types of bacteria‚ gram negative and gram-positive. The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number 15. The following are the tests that were used to identify the two different bacteria. The SIM test‚ which tests for sulfur reduction‚ indole production‚ and motility‚ MRVP which is
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Name SOLUBILITY CURVES Answer the following questions based on the solubility curve below. Which salt is least soluble in water .. at 2O° C? 2. How many grams of potassium chloride can be dissolved in 200 g of water at 80° C? IO 3. At 40° C‚ how much potassium _ __nitrate coin be dissoiu$tl ^n 30D.g of water? ------W- ’1 80 70 ...- O --60 0 5© 40 4. Which salt shows the least change 30 In solubility from 0° - 100° C? 20 10 At 30° C‚ 90 g of sodium
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Escherichia coli is a rod-shaped Gram-negative bacteria from the Enterobacteriaceae family‚ which owns the usual flora and can be be as pathogenic Human IN DUE can be cause diarrhea. The rational NOT USE ON antibiotics Infectious diseases can be causing the occurrence of resistance so that the necessary alternative to using Active Ingredients antimicrobial From medicinal plants. Turmeric (Curcuma domestica Val.) It is a prayer One Crop Growing in Indonesia That can be used as a medicinal plant. BECAUSE
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Introduction:The objective throughout the gram staining experiment was to classify bacteria through their chemical nature as well as the physical structure of their cell walls. Through this experiment one would be able to observe either a thick or thin layer of peptidoglycan by the appearance of the bacteria staining either gram-positive blue-purple or gram-negative pink. Question: Is there a subsequent effect of the bacteria when decolorizing with alcohol? Hypothesis: If there is either a removal
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Gram Stain: The important part of this experiment is being able to determine a bacterium based on its cell wall structure. It also helps indentify if the unknown organism is a Gram positive or Gram negative. This is the initial step that must be taken before any other lab procedure may continue on to ensure the purity is present‚ the arrangement of the cells‚ and the shape the cell has. The staining of the cell starts off with using the primary stain‚ Crystal Violet which is a purple color‚ to begin
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T-φ gram Determination of Unreported Meteorological Quantities from T-φ gram 1. Mixing Ratio (W):- It is the ratio of the mass of water vapour to the mass of dry air containing the vapours at a certain temperature. It is expressed in gm/kg. On T-φ gram from dew point temperature we follow isohygric doted green line and read the value at the base. (It can be calculated at all levels where dew point temperature is available). 2. Saturation Mixing Ratio (Ws) :- Mixing ratio at
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INTRODUCTION This scientific report is to discuss the results of an experiment being conducted on the effect that antibiotics have on the growth of bacteria. This will be done through the use of microbiology. Microbiology is a science in the department of biology‚ particularly microorganisms. These include; bacterium‚ fungi‚ protozoa‚ algae and viruses. This is an important subject to study as microorganisms can be found everywhere‚ covering all matters of different surfaces even including the human
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Antibiotics are used for treating human infections that are caused by specific bacteria‚ fungi‚ protozoans‚ and other large viruses. They are generally grouped with the class of microorganisms that they inhibit. Antibiotics are used to fight off infections within the human body system by inhibiting the growth of its infectious microbes. Antibiotics have been considered one of the greatest newest innovations in medicine but there is still yet to be a perfect ideal antibiotic. In this experiment six
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Lab 4: Cell Structures and the Gram Stain Purpose: The purpose of this lab is to familiarize the student with the use of a compound microscope and the Gram Stain test. Students will review a brief video and slides to introduce the compound microscope. Students will also view a video about the Gram Stain procedure and then answer questions related to it. Hypothesis If I look at the slides I will see where the cell wall is and what lies inside it and in the Gram Stain if I watch the video I will
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