Bacteria‚ such as Vibrio natriegens‚ are single cellular‚ microscopic microorganisms. Bacteria grow by cell division‚ mainly by a process called binary fission‚ where two cells arise from one single cell (Madigan et al.‚ 2015). In bacteria such as Vibrio natriegens‚ who are curved-rod shaped microorganisms‚ they elongate to almost twice their own size and form a dividing wall in which splits the single cell into two daughter cells (Madigan et al.‚ 2015). There are four phases to bacterial cell growth:
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of the unknown bacteria lab assignment was to select an unknown bacteria culture and‚ through a series of metabolic tests‚ identify which bacteria genus resided in the pure culture received. A nutrient broth inoculated with bacterial culture (numbered 45‚ henceforth referenced as U45) was selected and a streak plate was made to isolate a pure culture for use throughout the assignment. From the streak plate‚ several slides were made to determine the morphology of unknown 45. A Gram stain‚ used to
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Jennifer Hauss March 4‚ 2015 Bacterial Transformation Lab Report Introduction In this lab‚ the goal was to transform the bacteria e-coli to glow in the dark (or under a black light). Four plates were set up with agar in them for the bacteria to feed on and grow. Changes were then made to the bacteria. One plate was the control plate‚ having only the LB or agar for the bacteria and negative pGLO‚ which is the liquid not containing the plasmid. This is the plate that was compared with the three
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The Gram stain is one of the most useful staining procedures in microbiology . It is one of three differential staining techniques‚ is used to identify divide bacteria into two groups: Gram-positive bacteria and gram-negative (Madigan‚ Martinko‚ Dunlap‚ Clark (2009). These staining reactions take advantage of the fact that cells or structures within cells display dissimilar staining reactions that can be distinguished by the use of different dyes (Brown 2009). A gram positive bacteria can be
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Hydrogen Producing Bacteria was incubated in a complete - mix digester with work volume 1.7 L‚ seeded with sludge obtained from the local sewage treatment plant. Each liter of feed medium was composed of the following : 7 g of glucose‚ 1 g NaHCO3 ‚ 500 mg of NH4Cl ‚ 250 mg KH2PO4 ‚ 250 mg K2HPO4 ‚ 320 mg of MgSO4 • 7H2O ‚ 50 mg of FeCl 3 ‚ NiSO4 32 mg ‚ 50 mg CaCl2‚ Na2BO7 7.2 mg H2O ‚ 14.4 mg (NH4) 6MO7O24 H2O ‚ 23 mg of ZnCl2 ‚ 21 mg CoCl2 H2O ‚ 10 mg CuCl2•2H2O and 30 mg of MnCl2•4H2O . The reaction
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and Gram Staining Method Results Table 1. Gram stain reaction and cellular features of the culture. Gram staining methods were applied on the given mixture of Bacillus cereus‚ Escherichia coli and Staphylococcus aureus and then examined microscopically. Results were recorded in Table 1. Gram Reactions Cell shapes Cell Ends and Arrangement Size Distinctive Characters Predicted Bacteria Bacteria 1 Gram positive (purple) Cocci Rounded‚ clusters‚ singly Small - Staphylococcus aureus Bacteria 2 Gram
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Experimental Part 1: Reaction First the glassware apparatus for the reaction was set up. 3.861-grams of isoborneol‚ 2.21-mL of glacial acetic acid‚ and 4.39-mL of 6% NaOCl solution were mixed in a 125-mL Erlenmeyer flask. Another 35-mL of 6% NaOCl solution was added to a separatory funnel and supported over the flask. The NaOCl in the sep funnel was slowly added into the Erlenmeyer flask with vigorous swirling‚ approximately 2-mL every 30 seconds until all the NaOCl from the sep funnel had been
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Lab: Sampling Bacteria Purpose: Refer to handout sheet. Materials: Refer to handout sheet. Procedure: Refer to handout sheet. Pre-Lab Questions: 1. Why is one dish being reserved for the class as a "control"? Having a controlled variable is important in order to be able to look at what the bacteria would look like if it hadn’t been contaminated and just left as agar. Having a sample of agar that wasnt exposed to any bacteria will provide a clear picutre of what grew on the agar
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1. Obtain 1 gram of an impure‚ unknown substance solid. Make sure to stir the mixture before measuring the sample; record the mixture’s code in the data section. 2. Add approx. 25-50 mL of water and several boiling chips to a 125 mL Erlenmeyer flask and heat the water to a gentle boil using a hotplate. 3. On a second hotplate‚ place a 125 mL Erlenmeyer flask containing the one gram of unknown solid along with a boiling chip. 4. Using a ring clamp‚ slowly pour approx. 5-10 mL of boiling water into
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lifetime (47 years of full-time work) this gap amounts to a loss in wages for a woman of $700‚000 for a high school graduate; $1.2 million for a college graduate and $2 million for a professional school graduate. (Census Bureau reports and data‚ Current Population Reports‚ Median Earning of Workers 15 Years Old and Over by Work Experience and Sex. Updated September 2009) from a feminist prospective‚ I think this discrimination exists‚ because women’s role is considered to the homemaker and caregiver
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