bacteria. Learning how to gram stain‚ use specific media such as MacConkey agar‚ and test antibiotics to see which antibiotic would react best against a specific organism. All these techniques helped me prepare for the final lab‚ identification of an unknown bacterium. For the final lab‚ I received the organism “6A”. To start identifying this organism‚ I did a gram-stain to identify if the organism is gram positive or negative. I created two slides to ensure that I did the stain correctly with similar
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The microbiology lab up to this point has been used to teach the students how to stain and identify bacteria. There are several types of staining through which the bacteria can be identified based on the color and shape. The staining methods used in the lab are Gram Staining‚ Capsule Staining‚ Endospore Staining‚ and Acid Fast staining. One of the most significant method of staining is the Gram Staining‚ as it is highly dependent (McCarthy‚ 25). In the specific experiment that was done‚ Gram Staining
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UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. This is important in the medical field because identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease. Also knowledge of different bacteria helped others
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eosin Y and methylene blue found in the medium inhibit the growth of gram-positive bacteria but not the growth of gram-negatives. Lactose fermenters metabolize the lactose in the media and produce acid byproducts‚ causing a color change in the colony which is dark purple‚ almost black. Strong acid productions by organisms result in a metallic green sheen. Weaker fermentation of
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Gram Staining Lab Introduction Gram staining is a very important technique used in biology labs all over the world. It is a technique used to differentiate types of bacteria using certain physical and chemical characteristics of their cell walls. Gram positive bacteria (which show up purple after the gram staining process) have a very thick layer of peptidoglycan where gram negative bacteria (which show up pink after the gram staining process) have a much thinner layer of peptidoglycan. One thing
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Page I - Cover sheet In the middle f the page give name and number of your microorganism In the right lower corner provide - your name - Lab section number (Biol 108-005) - Date submitted ( 4/18/2013) - the unknown tube # is 5 Page II table of result - This page will have your table of results include the following information - Name of the test - Medium used - Indicator used - your results Part III - All the test done As many pages as needed to do a complete job. in this section
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INTRODUCTION The Gram Negative lab had two parts; the aim of the first part is to determine the concentration of gram negative bacteria in a water sample collected from a creek near Providence Road‚ Strickling. Gram negative bacteria have a cytoplasmic membrane‚ a thin peptidoglycan layer‚ and an additional outer membrane composed of phospholipids and lipopolysaccharides. Because gram negative bacteria have a relatively thin cell wall when compared to gram positive organisms‚ they are consequently
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bacterium was handed out by the lab instructor. The methods that have been learned so far in identifying bacteria were applied to this unknown. Procedures were followed as stated in the lab manual and biochemical test handouts. The first procedure that was done was a gram stain followed by a streak of the unknown on a TSA plate in order to determine the gram reaction and observe the colony morphology. After that‚ specific biochemical tests were performed for gram positive‚ since unknown number
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WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY GENERAL Unknown reports in microbiology are written in scientific format. Scientific writing is written differently from other types of writing. The results of the exercise or experiment are what are being showcased‚ not the writing. The purpose of scientific writing is not to entertain‚ but to inform. The writing should be simple and easy to understand. There is a specific style that must be followed when writing scientific reports. Scientific
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Lab12-Medical Microbiology- Part1– Differential Media/Biochemical Tests‚ Sp2012 (Set all of your margins to ½”) Purpose: The purpose of this lab is to help you become a little familiar with some of the tests that can be typically performed in a clinical or research lab facility. These tests may help in determining a particular pathogen’s growth needs. There are several sections to this lab. Find each section and complete the “Preparing for Class” sections. Preparing for class - Day 1 Read
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