Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
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Wave Nature of Light Objective: The purpose of this lab is to investigate interference‚ otherwise known as the diffraction of light. A beam of light acts a wave‚ and we are able to use equations so calculate the wavelength of the light used. The diffraction of a straight edge demonstrates that light waves bend around straight edges‚ allowing light to enter an area of shadow. When waves are superposed‚ they reinforce each other when crests are in phase and cancel out when they are not in phase
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PEES 4200W- Physiology of Exercise Lab #2: Metabolism and Energy Expenditure 1. The Respiratory exchange ratio is the ratio of the volume of carbon dioxide produced and oxygen consumed. The ratio indicates the energy that the subject is expending for indirect calorimetry‚ how efficient the subject ’s body is at utilizing the oxygen inhaled‚ as well as the main substrate being used for energy during varying intensities of exercise. During rest the volume of carbon dioxide was 0.73L/min‚ the volume
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LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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Nervous and Endocrine System Work Sheet View the following case studies on the Anatomy and Physiology place website: Chapter 7 –Nervous System; Chapter 8- Special Senses Chapter 9 – Endocrine Try to answer the questions in the case study and be sure to check the correct answers! However you will not need to submit your answers to these questions‚ only the questions on the worksheet ( see below). Submit your answers as a word document to the Week 4 dropbox 1. Define a reflex and list
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reasoning processes‚ memories‚ and biological activities that maintain bodily functioning 3) Psychologists describe‚ explain‚ predict‚ and improve behavior in every aspect of their jobs. 4) Behavioral neuroscience focuses on how the brain and nervous system functions as they relate to behavior. 5) Which of the following subfields in psychology focuses on the processes of sensing‚ perceiving‚ learning‚ and thinking about the world? Experimental physcology 6) Which of the following subfields in
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Nervous System I: Ion Channels 1. What structures in the cell membrane function as ion channels? Integral Proteins 2. Ion channels are selective for specific ions. What three characteristics of the ions are important for this selectivity? 1. Charge 2. Size 3. Amount of water the ion attracts 3. Channels can be classified as either gated or nongated channels. A sodium channel that is always open would be classified as a/an __________nongated______________ channel. 4. Would sodium ions
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chemicals before use. Abide by specific warnings and directions. 3. Collect all materials needed for a procedure before proceeding. 4. Perform reactions under the hood when directed. Chemicals may be weighed and prepared at balance or lab tables‚ but tests should be carried out under the hood. 5.Acids and caustic chemicals are stored in the hood. Please do not take these chemicals from the hood. Procedure: PART 1: Metathetical reactions Precipitation reactions A1. Add a
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Organic Chemistry II Lab 9 Fermentation of a Carbohydrate: Ethanol from Sucrose * Introduction Ethanol is one of the oldest alcohols and also the least toxic one. Industrially‚ ethanol is made most economically by hydration of ethylene. However‚ ethanol that is intended for human consumption must‚ by law‚ be prepared by fermentation. By either method‚ ethanol‚ of course‚ has the same formula‚ structure‚ and properties. The fermentation takes place with the assistance of enzymes from yeast
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across fittings in a closed conduit to find out the loss coefficients of these fittings. Thereafter‚ we determine the individual flow rates of the 3 different lines in the system. With this knowledge‚ sufficient understanding about the properties and characteristics of the different devices that can be used in a piping system is necessary for the choice of the appropriate devices to be used in future experiments. In the second part of the experiment‚ we observe how the flow rate of a fluid changes
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