University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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Results: Since the mass of the ball bearing used in this experiment was very light‚ 20 balls were measured and the result was divided by 20 for better accuracy. This procedure was repeated for the sizes and the results are as follows: Diameter (mm) Mass (kg) 2 0.04 3 0.075 4 0.11 To find out the densities of the three balls‚ the following formula was used: Density= (mass of the ball)/(Volume of the ball)= mass/(4/3 π r^3 ) Density of 2mm ball = (0.04⁄1000)/(4/3 π 〖(0.001)〗^3
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Unknown Report Emily Juhl Section – 3 April 15‚ 2013 Unknown #8 Family: Enterobacteriaceae Genus: Serratia Species: Marcescens Abstract Microorganisms are constantly finding ways to resists to antibiotics. For this reason‚ it is important to test and observe unknown organisms in the lab to continually improve the health and well being of society. The objective of this report was to first isolate a single colony of an unknown culture on a LB agar plate. From
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Radial Immunodiffussion (RID) Christian Crespo 18 October 2013 Immunology Lab Report Purpose of the Experiment: The objective of this experiment is to quantitatively observe the foundational reaction in our Immune system; the Antigen-Antibody interactions. The Ouchterlony procedure is what will be used in this lab to detect nature of the antibody interaction. The orientations of the band will provide more information about the interaction of antibody and antigen
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4/29/2013 5.1 Lab Report - Identification of Metallic Ions TITLE: LAB 5.1 IDENTIFICATION OF METALLIC IONS PURPOSE: In this lab we are learning how to observe and perform “flame tests”‚ of alkali and alkaline earth metal ions. We will use basic lab equipment provided in our Labpaq‚ and follow all safety precautions in this lab. From reading the discussion and review‚ we understand that the normal configuration of the electrons is called “the ground state”. We understand that they are
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Gut Bacteria In today’s world‚ many people have a very negative connotation when they hear the word “bacteria”. Bacteria have been the cause to many diseases‚ however bacteria do have some benefits and uses. There are thousands and thousands of different types of bacteria in the world and one group of bacteria‚ Gut Flora‚ has become evident in the bacterial community for being beneficial. Gut Flora is a group of bacteria that consists of microorganisms that live in the digestive tracts of animals
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Planarian Lab Report Hanna Klein Bishop Fenwick High School Planarian Lab Report Introduction Planarian worms are free-living flatworms that are not parasitic. Planarians have a three-cavity digestive system. Planarians are usually either carnivorous or scavengers. Planarians may eat other living‚ as well as dead‚ invertebrates‚ detritus or decaying organic matter. Planarians do not realize that are eating a lot‚ and can over eat resulting in death. Most planarians are freshwater forms that can
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bacterial inhibitors abolish the most bacteria. In the future‚ people will choose the most effective bacterial inhibitor; therefore‚ they save both money and time. If the disinfectants are applied to the bacteria‚ then the zone of inhibition will increase because disinfectants are antimicrobial agents that are applied to non-living objects‚ and a petri dish is a nonliving
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is researching a newly-discovered species of bacteria. At time t = 0 hours‚ he puts one hundred bacteria into what he has determined to be a favorable growth medium. Six hours later‚ he measures 450 bacteria. a. Assuming exponential growth‚ what is the growth constant "k" for the bacteria? (Round k to two decimal places.) b. After 10 hours‚ how many bacteria will there be? c. When will there be 10‚000 bacteria? 10. A certain type of bacteria‚ given a favorable growth medium‚ doubles in
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Chromatography lab Purpose: To separate food colorings into their component dyes using paper chromatography. Materials: Chromatography paper‚ Food coloring‚ Ruler‚ Pencil‚ Solvent solution‚ Test tubes‚ Test tube rack. Safety precaution: wear aprons‚ to make sure that you don’t get any of the alcohol on your clothes‚ and if you break a test tube you don’t get glass on you. Procedure: See-attached handout. Results: See chromatography with Audrey’s lab report.
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