LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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Robert Lawrence and Edmund Stephens‚ two graduate students of geology from Columbia University‚ organized the Saline Salt Company in 1974. Keeping in mind the fact that every 1‚000 grams of seawater contains approximately 35 grams of various salts‚ the two men pictured limitless wealth for them by extracting these salts. Common table salt comprises 27 grams out of each 35 grams and is easy to extract; so‚ the men decided to begin producing it. They picked New Iberia‚ Louisiana as the ideal location
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Theoretical Background 4 D. Experimental Procedures 9 E. Results and Calculations 12 F. Discussion 50 G. Error Analysis 58 H. Conclusion 61 Part II: Centrifugal Pump Characteristics I. Introduction 62 J. Objectives 63 K. Theoretical Background 63 L. Experimental Procedures 68 M. Safety Precautions 68 N. Results and Calculations 69 O. Discussion 75 P. Error Analysis 79 Q. Conclusion 80 References 81 Appendix I.A Appendix II.A Appendix II.B Summary There are two parts
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The Charge/Mass (e/m) Ratio of the Electron PHYS 0212: Introduction to Laboratory Physics Fall 2012 Abstract The experiment conducted demonstrated correlation between the charge and mass of an electron and the behavior of magnetic fields. The lab was divided into four parts. The first three parts were conducted with a compass that was used to locate the magnetic field lines around a bar magnet‚ a solenoid and then a pair of Helmholtz coils. As a result of these trials‚ our observations found
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Organic Chemistry II Lab 9 Fermentation of a Carbohydrate: Ethanol from Sucrose * Introduction Ethanol is one of the oldest alcohols and also the least toxic one. Industrially‚ ethanol is made most economically by hydration of ethylene. However‚ ethanol that is intended for human consumption must‚ by law‚ be prepared by fermentation. By either method‚ ethanol‚ of course‚ has the same formula‚ structure‚ and properties. The fermentation takes place with the assistance of enzymes from yeast
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If sodium chloride is added to a solution with Saccharomyces cerevisiae‚ then sodium chloride should hinder the growth and reproduction of those cells because the salt concentrations will limit the overall efficiency of the cell’s reproductive processes as well as decrease overall colony size. In our experiment‚ we plan to note the effects of salt on S. cerevisiae by both spot plating to note overall cell colony size and the number of total cells and through the use of electro photometry to also
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Separation of the Components of a Mixture General Chemistry 1 (Chem 101)‚ ISP SCUHS Report 2 January 26‚ 2014 Abstract The analyses of mixture were to distinguish and identify homogeneous mixture by using the techniques of decantation and sublimation. By performing these techniques‚ we examined our solutions such as SiO2 (sand)‚ NH4Cl (ammonium chloride)‚ and NaCl (sodium chloride) and mixed H2O (water) with each solution after being heated. After examining our solutions‚ we made calculations
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concentrations of solutes in and out of the cell. Filtration is what is used to remove solid particles and they can be removed by passing through a liquid and gas. The information above is very important because it is exactly what everything in this lab will be about. It explains in detail what every single little definition and important
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Neurophysiology Lab Report Anatomy & Physiology Lab Report Exercise 3 Activities 1-4‚ 8 By Laurence Blake 2/27/12 A. Objective I. Activity 1-4: Eliciting a Nerve Impulse • Investigate what kinds of stimuli stimulate action potential. II. Activity 8: Nerve Conduction Velocity • Determine and compare the conduction velocities of different types of nerves. B. Introduction I. Activity 1-4: Eliciting a Nerve Impulse • In this experiment‚ we
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Job Safety Analysis or Job Hazard Analysis All employers have duty of care to individuals‚ staff‚ contractors or third parties in the workplace. It is also a legal requirement that organizations should document any health and safety risks that have been identified in the workplace. Equally‚ it is necessary to record the actions taken to reduce those risks What is a Job Safety Analysis or Job Hazard Analysis? One way to increase the knowledge of hazards in the workplace is to conduct
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