LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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Membrane Processing MEMBRANEFILTRATION A membrane or‚ more properly‚ a semipermeable membrane‚ is a thin layer of material capable of separating substances when a driving force is applied across the membrane. Membrane filtration enables to separate particles with a diameter smaller than the pore diameter in the membrane from the liquid feed‚ by applying a driving force (pressure) over the membrane. The membrane technology consists principally of Microfiltration (MF)‚ Ultrafiltration
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Lab Report An enzyme is a protein that speeds up the rates of chemical reactions. They recognize‚ bind‚ and change specific reactants. They do not change so they can catalyze the same reaction again and again. Activation energy is the amount of energy needed in order to begin a chemical reaction. A Catalyst is a substance that increases the rate of a chemical reaction without itself undergoing any permanent chemical change. Catalysts are substances or a substance that configures another substance
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surrounded by a plasma membrane. The plasma membrane functions somewhat like a wall‚ as it keeps the internal contents from the external environment. Just like a wall‚ the membrane is also somewhat permeable‚ except that the membrane takes a much more active role in determining what is allowed in to the cell and what is kept out. The plasma membrane is a very thin structure‚ which has some very important tasks. One of the main tasks that it performs is to control the movement of foreign substances
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There are many branches of food science‚ all of which study different aspects of food such as safety‚ microbiology‚ preservation‚ chemistry‚ engineering‚ physics and the like. Until the advent of molecular gastronomy‚ there was no formal scientific discipline dedicated to studying the processes in regular cooking as done in the home or in a restaurant. The aforementioned have mostly been concerned with industrial food production and while the disciplines may overlap with each other to varying degrees
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processes that account for the movement of substances across the plasma membrane‚ and to indicate the driving force for each. This may also be applied to the study of transport mechanisms in living membrane-bounded cells. Also‚ understanding of which way substances will move passively through a deferentially permeable membrane depending on the concentration differences. We used PhysioEx software to examine diffusion. In these experiments we used different sized membranes as well as NaCl‚ urea‚ glucose
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Osmosis is a passive movement of water through a selectively permeable membrane moves from an area of higher concentration to an area of lower concentration. In this lab‚ we can determine the direction of diffusion of small molecules by measuring the diffusion of small molecules through dialysis tubing. This tubing acts as a selectively permeable membrane‚ and allow to pass larger molecules slowly. Introduction: Cells have kinetic energy and it causes the molecules of the cell to move around
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Exercise 1: Cell Transport Mechanisms and Permeability: Activity 1: Simulating Dialysis (Simple Diffusion) Lab Report Pre-lab Quiz Results You have not completed the Pre-lab Quiz. 09/20/11 page 1 Experiment Results Predict Question: Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through the 20 MWCO membrane? Your answer : c. No‚ not at all. Predict Question 2: Recall that glucose is a monosaccharide‚ albumin is a protein with 607 amino acids‚ and
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Lab Report 1 Introduction: Proper chemical formulas entitle many challenges such as the Law of Multiple proportions that states that there may be more than one plausible mole ratio for the elements in that compound. However if we determine the mass of each element in the compound we will be able to get the true chemical formula. In this experiment‚ we used the law of definite proportions to find the chemical formula for a hydrated compound containing copper‚ chlorine‚ and water molecules
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4.1 Aim The aim of this lab session is to demonstrate the effect of different conditions on membrane permeability. 4.2 Learning Objectives In this Lab Session‚ students will acquire the skills to: - analyze the effect of different temperatures on membrane integrity. - analyze the effect of the organic solvent acetone on membrane integrity. 4.3 Introduction Even though membranes separate cells from their surrounding environment and different organelles within the cells from the
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