LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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PEES 4200W- Physiology of Exercise Lab #2: Metabolism and Energy Expenditure 1. The Respiratory exchange ratio is the ratio of the volume of carbon dioxide produced and oxygen consumed. The ratio indicates the energy that the subject is expending for indirect calorimetry‚ how efficient the subject ’s body is at utilizing the oxygen inhaled‚ as well as the main substrate being used for energy during varying intensities of exercise. During rest the volume of carbon dioxide was 0.73L/min‚ the volume
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Instructor Biology 1111 4-5 Lab Topic 4: Microscopy Elodea Cells at ___X Elodea Cells at ___X Report Sheet—Lab Topic 4 1. Draw and label each of the organisms available. Cheek Cells at ___X Cheek Cells at ___X Name _______________________________ Date_____________ Instructor ___________________________ Section___________ _________________________ 4-6 Lab Topic 4: Microscopy 2. Fill in the following table: Compound Microscope Dissecting Microscope Types of Light Available Powers
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chemicals before use. Abide by specific warnings and directions. 3. Collect all materials needed for a procedure before proceeding. 4. Perform reactions under the hood when directed. Chemicals may be weighed and prepared at balance or lab tables‚ but tests should be carried out under the hood. 5.Acids and caustic chemicals are stored in the hood. Please do not take these chemicals from the hood. Procedure: PART 1: Metathetical reactions Precipitation reactions A1. Add a
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Organic Chemistry II Lab 9 Fermentation of a Carbohydrate: Ethanol from Sucrose * Introduction Ethanol is one of the oldest alcohols and also the least toxic one. Industrially‚ ethanol is made most economically by hydration of ethylene. However‚ ethanol that is intended for human consumption must‚ by law‚ be prepared by fermentation. By either method‚ ethanol‚ of course‚ has the same formula‚ structure‚ and properties. The fermentation takes place with the assistance of enzymes from yeast
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contained in a liter of water. If you put these organisms in a bottle and turn on the lights‚ you get photosynthesis. If you turn off the lights‚ you turn off the primary production. Darkness has no effect on respiration. This is because cellular respiration is actually the reverse process of photosynthesis. Oxygen is a necessity of life requirements for basically all living organisms.* In this lab we are testing how different levels of salinity in the water indirectly affects the gross primary productivity
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Introduction There are about a million different kinds of seeds in our world with all of the different types of plants that we have. One type of seed that takes between three to five days to germinate is a daikon radish. The radish (Raphanus sativus ) originated from China. It was commonly seen and eaten by the people of Egypt long before the Egyptians started to construct their very own pyramids. Later on‚ radishes became a common vegetable to eat in Greece‚ and since the radish meant so much worth
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concentrations of solutes in and out of the cell. Filtration is what is used to remove solid particles and they can be removed by passing through a liquid and gas. The information above is very important because it is exactly what everything in this lab will be about. It explains in detail what every single little definition and important
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“MALUNGGAY (Moringa oelifera) AND SPINACH (Spinacia oleracea) POWDER AS FOOD SUPPLEMENTS” RATIONALE He gives us that vast goodness of nature and vegetation is among those. As man propagates for abundance; he deserves to harbour tremendous benefits from it. Man inhabited earth with all its richness laid before him. With his gladness‚ ingenuity and constant search for knowledge he discovered numerous ways to enrich his mind of endless ideas‚ body for health and longevity and spirit to fulfil his
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Biology 160 Lab Manual Tacoma Community College Last update 06/03/2013 This page left intentionally blank Table of Contents Table of Contents i Laboratory 1: Principles of the Scientific Method 4 In-Lab Report Sheet 12 This page has been left intentionally blank 27 Laboratory 2: Scientific Methodology & Enzyme Activity 28 In-Lab Report Sheet 28 Objectives 33 Introduction 33 Parts of the Swift M5 Microscope 33
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