University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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Makenzie Ann Cox October 22‚ 2014 Biology 1112.317 Lab Report Amounts of Carbon Dioxide/ Oxygen Animals are Producing/ Consuming Introduction Every cell needs a source of energy in order to perform their daily functions. However the process to get this energy is different for plant and animal cells. Plants cells undergo a process called photosynthesis where light energy from the sun is used to convert carbon dioxide and water into glucose and oxygen. For animal cells‚ the process is known as cellular
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Factors Limiting Photosynthesis The rate of photosynthesis increases with increasing light intensity. But too much light intensity can slow down the rate of photosynthesis because the light damages chloroplasts in the leaf. The higher the temperature the greater the rate of photosynthesis as photosynthesis is a chemical reaction and chemical reactions increase with temperature. But at temperatures above 40 C the rate slows down because the enzymes involved are destroyed
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Radial Immunodiffussion (RID) Christian Crespo 18 October 2013 Immunology Lab Report Purpose of the Experiment: The objective of this experiment is to quantitatively observe the foundational reaction in our Immune system; the Antigen-Antibody interactions. The Ouchterlony procedure is what will be used in this lab to detect nature of the antibody interaction. The orientations of the band will provide more information about the interaction of antibody and antigen
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OVERVIEW OF PHOTOSYNTHESIS Autotrophs are plants that can feed themselves by making their own food. Photo-autotrophs are organisms that produce organic molecules from inorganic molecules using the energy of light. Producers are when plants make their own organic molecules. A redox reaction is oxidation and reduction. Photosynthesis takes water and carbon dioxide to make glucose and oxygen. Cellular respiration takes glucose and oxygen to make water and carbon dioxide. ● Chloroplasts have an outer
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Planarian Lab Report Hanna Klein Bishop Fenwick High School Planarian Lab Report Introduction Planarian worms are free-living flatworms that are not parasitic. Planarians have a three-cavity digestive system. Planarians are usually either carnivorous or scavengers. Planarians may eat other living‚ as well as dead‚ invertebrates‚ detritus or decaying organic matter. Planarians do not realize that are eating a lot‚ and can over eat resulting in death. Most planarians are freshwater forms that can
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Bacterial Transformation Lab Report Backround: The plasmid pGLO contains an antibiotic-resistance gene‚ ampR‚ and the GFP gene is regulated by the control region of the ara operon. Ampicillin is an antibiotic that kills E. coli‚ so if E. coli‚ so if E. coli cells contain the ampicillin-resistance gene‚ the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. Thus‚ transformed E. coli cells containing ampicillin-resistance
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Chromatography lab Purpose: To separate food colorings into their component dyes using paper chromatography. Materials: Chromatography paper‚ Food coloring‚ Ruler‚ Pencil‚ Solvent solution‚ Test tubes‚ Test tube rack. Safety precaution: wear aprons‚ to make sure that you don’t get any of the alcohol on your clothes‚ and if you break a test tube you don’t get glass on you. Procedure: See-attached handout. Results: See chromatography with Audrey’s lab report.
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Student Name: Amaan Rushdi Lab Partner Name: Dylan Course: BIOL 123 Lab Instructor: Megan Grandinetti Sowbug behavior on environment -lab report What area does the sowbugs prefer to choose for their dwelling? Abstract By working with physical isopods‚ ordinarily known as pill bugs‚ sow bugs or roly- polies‚ we tried whether these sowbugs favored a dark spot to a light place. Included in the lab are a few outlines and tables sketching out our aftereffects of the test. We measured recurrence
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[Name] [Teacher] [Course] [Date] Catalase Lab Report An enzyme is something that helps to speed up a chemical reaction. The enzyme changes from reaction to reaction‚ but it always has the same impact. However‚ certain variables may cause the enzyme to have a more or less significant impact on the speed of each reaction. One of these variables that changes the effectiveness of an enzyme is temperature. There is an optimal functioning temperature for each enzyme in each reaction‚ depending on
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