Title: Rate of Photosynthesis Introduction: Photosynthesis occurs in plants where the light energy changes to chemical energy. This process occurs in the chloroplast of the plant cell in order to have gas exchange in the organism. Purpose: To measure the effect of light intensity on the rate of photosynthesis in the leaf cells. Hypothesis: The higher the concentration of the sodium bicarbonate solution‚ the faster the leaf of the plant will rise to the surface of the water in the beaker‚ the
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between light intensity and photosynthesis that could normally be seen had the experiment taken place without errors. The graph should show an increase in arbitrary units the further away from the light source the beaker is because the greater the distance from the light source‚ the lower the light intensity and so less photosynthesis would take place in the algal balls. This would result in more CO2 being present because it wouldn’t be being used up during photosynthesis. The decrease in CO2 in the
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BIOLOGY DCN PHOTOSYNTHESIS LAB # 6 Effect of varying coloured filters on the photosynthetic rate of spinach chloroplasts The data below is just representative of what trends and relationships you were supposed to see. Numbers can vary. Absorbance at 620 nm for each treatment DCPIP + chloroplasts t=0 min. 0.93 0.945 0.905 0.915 t=3 min. 0.95 0.731 0.83 0.816 change in A620 -0.02 0.214 0.075 0.099 t=0 min. t=3 min. change in A620 t=0 min. t=3 min. change
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The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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Method: as in practical guide results: Experiment 1 calibration curve- Table showing the absorbance and concentration of the 10 dilutions of p-nitrophenol at 405nm Tube no | blank | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 150 µM
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The results of the food coloring exercise are seen in Figure 1 of the group report. The red food coloring displayed the highest percent transmittance in the long wavelengths closest to red light‚ which was expected. With the same logic‚ it was expected that the blue food coloring would have the highest percent transmittance in
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Misael Acosta Photosynthesis Lab Report Ms. Rocha AP Biology 4th Period II. Intro/Background 1. In order for oxygen to be present photosynthesis must occur. Photosynthesis takes in light and CO2‚ and through a series of steps produces oxygen. Photosynthesis can be measured by either the disappearance of substrate or the accumulation of product. Examples include the production of O2 or the consumption of CO2. All the oxygen within the leaf disks will be removed before being placed
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This experiment is concerned with identifying photosynthetic pigments found in spinach plants and determining the spectrum of light each absorbs. By using paper chromatography‚ different pigments of spinach leaves can be separated. The knowledge gained in this experiment is relevant to understanding how the process of photosynthesis works. A Real-world application for this includes the harvesting of clean energy sources‚ as scientific advances have led the way to artificial photosynthesis on the
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Photosynthesis is a crucial energy conversion process that occurs in the chloroplasts of eukaryotic cells. The rate of photosynthetic activity holds importance in environmental‚ horticultural and agricultural situations. (Boardman‚ N.K. 1977). Pigments in the chloroplasts thylakoid membrane absorb the electromagnetic radiation from a light source and release an electron into the electron transport chain. DCPIP’s chemical ability to favourably accept electrons‚ and undergo a colour change from
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The purpose of this lab was to investigate whether veins affect photosynthesis rate. It was predicted that having veins would decrease the rate because chloroplast might be less frequent due to the vascular tissue. For this experiment‚ 4 groups were set up: veins and no veins in CO2 solution‚ and veins and no veins in distilled water‚ the distilled water groups served as controls. These 4 groups were placed under a light bulb in order for photosynthesis to occur. No disks experience movement in the
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