The Kinetics of α-Chymotrypsin Introduction Chymotrypsin is a protease which cleaves proteins by a hydrolysis reaction‚ it does this by adding a molecule of water to a peptide bond. Although the hydrolysis reaction is thermodynamically favoured in the absence of a catalyst the half-life for a typical hydrolysis reaction by a protease is between 10 and 100 years‚ needless to say it is extremely slow1. Though this is true peptide bonds are hydrolysed within milliseconds in the body in the presence
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Abstract: In this Lab we used the chemical DPIP to detect the rate of succinate broken down by the mitochondrial solution. We detected the amount of DPIP in the solution with a spectrophotometer and measuring the absorbance of light at the 600nm range. DPIP is a useful chemical to use in this experiment because it goes from a blue color when oxidized to a colorless liquid (Ogura‚ 281)‚ this is due to the hydrogen ions and electrons released during the transitional step between succinate and fumarate
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blessed with biodiversity composed of prokaryotes and eukaryotes. Almost all of them have specialized systems of sensing light‚ sensory and/or motor response composed of a cell‚ tissue or organ like eyes and brain. The Pax (Paired box) genes and proteins have been observed critical during formation of such tissues or organs and Pax family transcriptional regulators remain critical to maintain their functional anatomy. The Paired Box 6 (Pax6) best exemplifies the conservation of functions through
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during the lab session Procedure Part A This lab was performed by the group members as they closed both low and high flow rotameters (Fig-2) and filled the tank with water. Then‚ the large rotameter was opened when the water in the tank had reached the internal baffle‚ so the flow from the tank was reduced by doing so. One member was in charge of adjusting the level of the water so it was always maintained over the baffle’s level during the lab (Fig-1). The first part of the lab was done
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Lab Report Marisa McKinney P. 2 Purpose: To investigate the periodic variation of density in Group 4A elements. Background: When the elements are arranged in order of increasing atomic number‚ they exhibit periodic recurrence of properties. Elements in the same group in the periodic table tend to have similar physical and chemical properties. These similarities are due‚ in large part‚ to similarities among the electron configurations of the elements in a group. You can find periodic
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"18. Dehydration of 2- Methylcyclohexanol." Organic Chemistry Lab 2040L. XU Chemistry‚ n.d. Web. 12 Feb. 2013. 2. "Material Safety Data Sheet 2-Methylcyclohexanol." Http://www.coleparmer.com/Assets/Msds/97403.htm. Coleparmer‚ 19 Mar. 1998. Web. 12 Feb. 2013. 3. "Material Safety Data Sheet Methylcyclohexane." Sciencelab.com. Science Lab‚ 09 Oct. 2005. Web. 12 Feb. 2013. 4. Helmenstine‚ Anne M.‚ Ph.D. "How to Write a Lab Report." About.com Chemistry. About.com Guide‚ n.d. Web. 23 Feb. 2013
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Experiment 2: Food Tests Objective * To study the presence of reducing sugars. * To study the presence of protein. Introduction In this experiment‚ glucose‚ maltose‚ lactose and sucrose are used for testing reducing and non-reducing sugars. Glucose is monosaccharide while maltose‚ lactose and sucrose are disaccharides of carbohydrates. Monosaccharaides are the monomers which make up all other carbohydrates and cannot be broken into smaller molecules by hydrolysis. Disaccharides are
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Crystallization: Lab Report Theory/Introduction The purpose of this lab is to understand the concept of purification through crystallization. Crystallization is the process of purifying a substance‚ with the most adequate solvent. The process of crystallization came to be based on the principle of solubility. First‚ we must choose a solvent. The solvent is crucial to crystallization because it’s what will allow impurities to dissolve. Therefore‚ when selecting a solvent it is important that it
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using a lamp and a spectrophotometer to measure the amount of light being transmitted through a test tube. Chloroplasts were placed into test tubes along with water‚ DPIP‚ and a buffer to neutralize the pH of the solution‚ and then placed under a lamp with a standard 40-watt light bulb. One test tube was used to calibrate the spectrophotometer so that each transmittance reading would be
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CO2 Lab Report Intro Alka-Seltzer tablets are used to treat cold/flu symptoms as it produces CO2 when dissolved in water. The goal of this lab is to calculate the amount of NaHCO3 is needed to produce enough CO2 gas to fill the room. Experimental One Alka-Seltzer tablet was weighed on the analytical balance. Then a piece was measured out to be between 0.21 and 0.28 grams. 500mL of distilled water was added to a 600mL beaker. 80mL of water was mixed with 20mL of 6M HCl in a 150mL beaker. A
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