Daniel Bergey Lab 2: Proteins and Starches Purpose The purpose of lab 2 and both tests with proteins and starches is to determine which substance contains either protein or starch. Hypothesis Proteins: I predict that any substance I test that derives from a living organism is will test positive proteins. Any substance that isn’t from a living organism more than likely will test negative for proteins. Starches: I predict that any substance that contains any level of glucose will test positive for
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3.7.2.1.5 Urea test PRINCIPLE Urea is hydrolyzed in the presence of urease to produce ammonia and CO2. The ammonia produced combines with 2 – oxoglutarate and NADH in the presence of GLDH to yield glutamate and NAD. Urea + H2O + 2H+ 2NH4+ + CO2 NH4+ + 2-Oxoglutarate +NADH H2O +NAD+ + Glutamate The decrease in absorbance due to the decrease of NADH concentration in unit time is proportional to the urea concentration. Ammonia
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10 Test Tube Mystery Introduction: The students were handed 10 test tubes with no identification. The students were supposed to design a method in determining what chemical was in each test tube. The list of chemicals is CuSO4‚ NH4Cl‚ NaOH‚ AgNO3‚ KI‚ H2SO4‚ NaBr‚ CaCl2‚ HCl‚ and Pb(NO3)2. The students are expected to determine the chemicals using physical properties‚ litmus paper‚ solubility‚ and the process of elimination. Physical properties‚ such as the color‚ can be determined by sight. Litmus
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In this lab‚ we tested the effect of flame on different chemical solutions. In order to do this‚ we used a wooden stick soaked in a metal and nonmetal solution‚ hovered it over a bunsen burner‚ and recorded the color that the flame changed to. The seven solutions we tested were lithium chloride‚ strontium chloride‚ calcium chloride‚ sodium chloride‚ barium chloride‚ copper chloride‚ and potassium chloride. However‚ we had another four additional solutions‚ which had unknown chemical composition
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Lab2: Testing for Proteins and Starches In this lab 8 total substances were tested to find out whether they are a Protein or a Starch. It is my belief that only 1 or two of each of the substances in test 1( proteins) and test 2 (starch) will test positive for either protein or starch. For this lab the following materials were needed to complete the experiments in test 1 for proteins: Di water‚ ev milk‚ 50% egg solution‚ 1% sucrose‚ 4 test tubes‚ 1 test tube rate‚ safety glasses‚ pipets and
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Purification of Green Fluorescent Protein Introduction: Transformation is used to introduce a gene coding for a foreign protein into bacteria. Hydrophobic Interaction Chromatography (HIC) is used to purify the foreign protein. Protein gel electrophoresis is used to check and analyze the pure protein. Research scientists use Green Fluorescent Protein (GFP) as a master or tag to learn about the biology of individual cells and multicultural organisms. This lab introduces a rapid method to purify
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specially. This funnel is called as V-funnel. This test is a self-compactibility test to measure the capacity of concrete to pass into the spaces. This test determines the viscosity and passing capacity of SCC. Fresh SCC is filled to the funnel without any compaction or vibration and then the sliding cover under the funnel is opened and the filling time of all concrete in the funnel to the pail is recorded. Fig. 2 V-funnel test L-box test L-box test is used to assess the passing ability of SCC to
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MEMORIAL UNIVERSITY OF NEWFOUNDLAND FACULTY OF ENGINEERING AND APPLIED SCIENCE Engineering: 4312 Mechanics of Solids I Lab Test #4 – Torsion Test OBJECTIVES: To carry out a torsion test to destruction in order to determine for a 1020 carbon steel rod specimen: 1. The modulus of rigidity‚ 2. The shear stress at the limit of proportionality‚ 3. The general characteristics of the torque‚ angle of twist relationship. REFERENCES: 1. Hibbeler‚ R. C. "Mechanics of Materials"‚ Prentice-Hall
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VO2 Max Test Objective of the test: To work out how much oxygen can be utilized during an intense exercise test‚ the maximum capacity of oxygen used in the body Background of the test: The aim of this study was to create and evaluate a submaximal cycle ergometry test based on change in heart rate ( HR) between a lower standard work rate and an individually chosen higher work rate. In a mixed population ( n = 143) with regard to sex (55% women)‚ age (21-65 years)‚ and activity status (inactive to
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Zebrafish Embryotoxicity Test (ZET) The ZET will be done following the method proposed by Beekhuijzen et al. (2015) with modifications. Six embryos will be exposed to each of the following treatments: 0.1‚ 0.05‚ 0.025‚ 0.0125‚ and 0.00625 mg/ml. Embryos will be incubated in 24-well plates with built-in lids. Each well will contain one embryo and 2 ml of the respective treatment. All test solutions will be renewed every 24h to maintain the integrity of the test concentrations. The embryos will
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