Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
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filters‚ allowing water‚ glucose‚ salt and waste materials to pass through to the Bowman’s capsule but preventing and red blood cells and plasma proteins to pass through. Reabsorption occurs in the proximal tubules of the nephron. Water‚ glucose‚ amino acids‚ sodium and other nutrients are reabsorbed into the bloodstream in the capillaries surrounding the tubules. Water moves via the process of osmosis: movement of water from an area of higher concentration to one of lower concentration. Tubular secretion
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Laboratory Report The Plasma Membrane I. Introduction The Plasma membrane is the edge of life‚ the boundary that separates the cell from its surroundings. It controls the traffic of materials in and out of the cell. (Reece‚ 2011). It is incredibly thin that is very vital in maintaining the integrity of the cell. Not only does the plasma membrane bind the other organelles‚ it also forms a dynamic structure which gives them their remarkable activity and selectivity. (Hickman‚ 2008). Diffusion
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listed below. You must observe and analyze these as part of the lab. The Iced Tea Debate The Salty Soup The Fire Bug 2 Use the DATA chart provided for recording observation based on three demonstrations you will watch 3 Watch each part of the experimental demonstration and make preditions about wht will happen in each scenario . Record your preditions and observations in the Data an Observations section of your labatory report format. Problem: How can matter and energy be described in
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Abstract In vitro studies show that tea tree oil can be used as a topical methicillin-resistant Staphylococcus aureus (MRSA) eradication regimen in a laboratory setting. This report has been researched using a wide range of journals and internet sources to find out whether tea tree oil can be used as an alternative to Mupirocin for those suffering from MRSA colonisation. The journals that were searched contained useful information about tea tree oil as an antiseptic‚ however‚ many of the studies
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Lab 3 : Techniques in Microscopy Title : Preparation of light microscope observe cell organisms. Objective : To observe and investigate the cell structure under the light microscope. Introduction A microscope is an instrument used to magnify and resolve a specimen. It is important to know several important features of microscopy which involves magnification‚ resolution and contrast. Magnification is the enlargement of a specimen while resolution is the ability to distinguish detail or the
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different microscopic visualization techniques as well as the different stages of mitosis. Then after we examined the crucial relationship between structure and function. Finally‚ the most efficient way of cleaning your hands and prevent bacterial growth‚ using was analyzed. The CHO cell samples were fixed; one with formaldehyde and another sample with maceration solution (ethanol/hydrochloric acid) and stained with orcein solution . In order to evaluate the levels of hygiene resulting from different
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Solute Concentration of Potatoes Lab #1 Purpose: To find the molarity/concentration of potato cytoplasm. Materials: As on page 1 in the lab handout. Procedure: As on page 1 in the lab handout. Data and Observations: Test Tube # | Concentration of sucrose solution (mol/L) | Initial Mass (g) | Final Mass | Percentage change in mass | 1 | 1.0 mol/L | 3.00g | 2.25g | -25.0% | 2 | 0.9 mol/L | 2.70g | 2.07g | -23.3% | 3 | 0.8 mol/L | 2.92g | 2.25g | -22.9% | 4 | 0.7 mol/L | 2.60g
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macromolecule content. The four macromolecules – lipids‚ proteins‚ carbohydrates‚ and nucleic acids – can be quantified and analyzed.
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Proteins are a diverse group of macromolecules with many different functions (Mbuthia‚ 2012). Many are structural components of bone‚ muscle‚ hair‚ tissue‚ etc. Others are enzymes that speed up cellular reactions. All proteins are made up of amino acids (Johnson‚ 2010). The purpose of this experiment is to determine whether peptide bonds are present in various food substances. The color violet will indicate the presence of proteins. The more intense the color is‚ the greater number of peptide bonds
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