Methicillin Resistant Staphylococcus Aureus Staphylococcus aureus is an important and common pathogen in humans. It is found in the nose or on the skin of many healthy‚ asymptomatic persons (i.e.‚ carriers) and can cause infections with clinical manifestations ranging from pustules to sepsis and death. Most transmission occurs through the contaminated hands of a person infected with or carrying S. aureus. MRSA infections frequently are encountered in health-care settings (Lowy‚ 1998). A common
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Cellular and Molecular Techniques - Lab #1 BIO 349 Lab Report #1 Microscopy and Staining Abstract The primary focus of this lab was on microscopy and simple stains. Microscopy that was used were magnification‚ slide preparation‚ and staining. Methylene blue‚ a simple staining component‚ was used to stain the slide in order to see the different microbes and determine their cellular shape. Introduction The purpose of this lab was to become familiar with the light microscope and how
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The acid-fast stain is performed on samples to demonstrate the characteristic of acid fastness in certain bacteria. Acid fastness is a characteristic that is shared by just a few organisms‚ so staining to determine if organisms possess this trait is useful in microbial identification schemes. The Ziehl-Neelsen method has endured as a reliable and effective way to demonstrate the acid-fast bacteria. Materials: 18-20 nutrient hour agar slant culture of Staphyloccus aureus 4 day old nutrient
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Method • Methodology is performed through 3 successive days: First Day: Trypan Blue cell viability assay: Description Trypan blue staining is a simple way to evaluate cell membrane integrity (and thus assume cell proliferation or death) but the method is not sensitive and cannot be adapted for high through put screening. Short 96 well assay: EACH condition should be done in triplicate or more. Steps 1. Trypsinize one T-25 flask and add 5 ml of complete media to trypsinized cells. Centrifuge
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Lab Report: Acid and Base Indicator Lab The title of my lab report is “Acid and Base Indicator Lab” this lab is testing what household solutions area bases depending on what color they changed. You have to use the pH balance scale to determine Acid‚ Base‚ and Neutral. On this lab we used 7 different solutions to determine if it’s either base‚ acid‚ and neutral. These are ammonia‚ vinegar; drain cleaner‚ soft drink‚ baking soda‚ detergent‚ and lemon juice are the solutions that are going to be
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Acid Rain Lab Report Introduction: The problem was to see how crushed chalk and whole chalk weather differently. The independent variables are The temperature of the liquids The surface area of the chalk‚ crushed vs whole The dependant variable was the weathering of the chalk; this was calculated by measuring the foam reactant. Procedure: Part B- For the two partners recording data with the room temperature liquids‚ the materials where collected first. Two of the graduated
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Lab Report Acids and Alkalis: Chemistry of Neutralization and Salt Formation Introduction: An acid is a group of chemicals. Acids are positively charged ions‚ they are liquid and are solutions of pure compounds in water. If you want to know if something is an acid‚ you can test it by using litmus paper. Acids will turn litmus paper red‚ whilst alkalis will turn it blue. Alkalis are negatively charged ions and are usually solid. Aim: To find out how much of different acids is needed to neutralize
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Acid Rain Lab #1 Chem. 1 20 February 2016 Concordia University Texas Angelina Luna angelinaluna97@yahoo.com Chem 1- Sect C Acid Rain Lab #1 Professor Koeck Abstract In this experiment our main objective was to create three gaseous oxides CO₂‚ SO₂ and NO₂ by using data that was collected using a pH sensor which was connected to a laptop with LoggerPro and Vernier. We created the gases by bubbling each gas into the water to create the three acidic solutions we were looking for. Once that
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from the Acid Rain (50% vinegar solution) did not allow any radish seeds to germinate. There were never signs of seed germination while observing the seeds over a three week period. In the petri dish labeled Acid Rain‚ 0 out of 10 seeds sprouted into plant growth as seen in Figure 5.2. The results from the effects of acid rain are compared on Figures 5.1 and 5.2.
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4 Strong Acid and Weak Base Lab PURPOSE: To find the molarity of an unknown acidic acid. MATERIALS: 1. 250 mL beaker 2. 10 mL graduated cylinder 3. 50 mL beaker 4. Funnel 5. 125 mL Erlenmeyer flask 6. 50 mL buret 7. Ring stand 8. Strong acid 9. Weak base 10. Phenolphthalein 11. De-ionized water. PROCEDURES: 1. Fill the buret with a weak base and place it in the ring stand. 2. Fill the 50 mL beaker with 30 mL of a strong acid. 3. Fill
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