LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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The Virtual Lab – ELISA Test Lab: Immunology 09/04/2013 Instructors: Dr. Charlie Wilson Written by: Dipen Patel I. Objective: The purpose of the lab was to learn the procedure of performing an ELISA test to determine whether a particular antibody is present in a patient’s blood sample. ELISA is an abbreviation for “Enzyme-linked Immunosorbent Assay." II. Introduction: The interaction of antigen and antibody outside the body can be used to determine if patient
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A buffer stock scheme is a form of government intervention designed to stabilize price. Governments apply buffer stock schemes to unstable markets‚ such as agriculture and commodities‚ where the ability and willingness of producers to produce fluctuates sharply. A buffer stock scheme stabilizes the price of a good by setting a ceiling/maximum and floor/minimum price for a good‚ e.g. rice. (Fig. 1). Price Band for Rice (Fig. 1) P S pmax
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1) Natural buffers are chemicals that the body releases into the blood stream to help maintain a healthy pH level. Carbon dioxide (CO2) acts as an acid by donating hydrogen ions when needed and forms carbonic acid when it dissolves in water. Carbonic acid bicarbonate is important for maintaining an acid base balance in the blood as it equalizes the pH (7.5) of the blood. All body fluids have buffers that defend the body against pH changes. A process that affects buffers in the blood is exercise
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Buffer Preparation (Gozani Lab) 1. 1 M Tris-HCl Buffers pH Volume (L) TrisBase (g) HCl (ml) pH 7.0 2 242.2 150-155 pH 7.5 2 242.2 120-125 pH 8.0 2 242.2 80-85 Autoclavable. 2. EDTA 0.5 M (pH8.0) 0.5M‚ 1L: 148 g EDTA + ~30-40 g NaOH to adjust pH (or 186 g EDTA-Na.2H2O + ~20 g NaOH) Note: pH adjusted by NaOH is essential for solubility. Autoclavable. 3. TAE DNA Electrophoresis Buffer (50 X) (2 M Tris‚ 50 mM EDTA) 2L 484 g Tris 114.2 ml glacial acetic acid 200 ml 0.5 M EDTA 8.0 To make
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Chem 105 Guide to the Formal Laboratory Report The purpose of a formal report is to communicate effectively to another person the goal‚ procedure‚ data analysis method‚ and results of your laboratory work. The report is divided into several well-defined sections. Each section must be present in a complete report. To earn an outcome point for the laboratory report‚ a student must submit a formal lab report that earns a score of at least 90/100. Each error (factual‚ grammatical‚ typographical
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Lab report is one way we used during of after an experiment in a laboratory to subtly record and discuss the experiment. During a lab‚ we sometimes can observe only the physical part of the experiment‚ or may be some visible chemical changes. These changes indicate that the experiment we do is successful or not. However‚ in order to understand and achieve more from just simply doing the experiment‚ we write lab report to more profoundly understand the internal meanings of the experiment we do‚ and
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Using ten pine boards 1.905 cm. by 2.54 cm. leaving 0.3175 cm. gaps between the boards forming a rectangular base held together with 1.27 cm. brad nails installed with a nail gun. Adding 4 boards on the outer perimeter with the nail gun. Forming the edge sides. Using a skill saw cut a 38.1 cm by 25.4 cm. rectangle out of your 0.635 cm. luan board (plywood). Repeat another time so that you have two of these boards. Using a skill saw cut a gable end at a 6-12 pits. Using a square to square the building
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Measurement of Capacitance using Arduino – An Interrupt-based Approach: Part I SAIKAT PATRA‚ SHIBENDU MAHATA Introduction Capacitive sensors are widely employed to measure various physical and chemical process parameters such as displacement‚ acceleration‚ thickness‚ force‚ pressure‚ stress‚ level‚ and humidity. The measured value of capacitance is then calibrated in terms of the process parameter for indication and/or control applications. This project presents an interrupt-based approach by
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CHEMICAL REACTIONS: ACID-BASE BUFFERS Short Overview Acids and bases represent two of the most common classes of compounds. Many studies have been done on these compounds‚ and their reactions are very important. Perhaps the most important reaction is the one in which an acid and base are combined‚ resulting in the formation of water (in aqueous solution) and a salt; this reaction is called neutralization. A buffer solution is a solution that contains both an acid and a salt
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