LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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PEES 4200W- Physiology of Exercise Lab #2: Metabolism and Energy Expenditure 1. The Respiratory exchange ratio is the ratio of the volume of carbon dioxide produced and oxygen consumed. The ratio indicates the energy that the subject is expending for indirect calorimetry‚ how efficient the subject ’s body is at utilizing the oxygen inhaled‚ as well as the main substrate being used for energy during varying intensities of exercise. During rest the volume of carbon dioxide was 0.73L/min‚ the volume
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Unknown Lab Report #1 Unknown #1 April 25‚ 2012 Microbiology Spring 2012 MCB2010C Unknown #1 Introduction Identity of a microorganism has proven to be very significant. Doing so can help identify diseases and created treatment and cures for such diseases. As a result‚ various laboratory tests were performed to an unknown microbe (Unknown #1) found in the water of a nearby pond. By identify the microbe‚ the safety of the water will be known to those around it. Materials and Methods
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Unknown Lab Report Dr. Nathan Cahoone Microbiology 204 December 9‚ 2010 Introduction There are many reasons for knowing the identity of microorganisms. The study and test was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium which I was using unknown #25. Results Unknown #25 had the following morphology on a streak plate: medium sized butyrous cream colored colony. Gram-staining was utilized
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-------------------------------- 1 A scientific paper submitted in partial fulfillment of the requirements in HNF 12 laboratory‚ 2nd sem.‚ 2012-2013. OBJECTIVES 1. To enumerate the principle and methods in fermentation; and 2. to identify the factors that affect the efficiency of fermentation. INTRODUCTION Vinegar has been traditionally used as a food preservative. Vinegar retards microbial growth and contributes sensory properties to a number of foods. The wide diversity of products containing
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absorbance of salt‚ dead yeast cells‚ contamination‚ plate overgrowth‚ and the yeast cells drying from being under the vent in the incubator. In the future‚ we can do this experiment more effectively by having more than three weeks to perform the experiment as well as letting the yeast grow for more than twenty-four hours. As a result of having a longer amount of time to do the study and letting the yeast grow for longer than twenty four hours‚ we would get better results; yeast multiplies and divides
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Department of biology Faculty of Science and Mathematics Universiti Pendidikan Sultan Idris LABORATORY REPORT SBT 1013: INTRODUCTION TO BIOTECHNOLOGY Semester 4 Sessions 2013/2014 ID NUMBER AND NAME 1. SITI NURFATINI BINTI MOHAMAD ROSLAN (E20131007560) 2.NURHAFIZAH BINTI SAMSUDDIN (E20131007584) 3.MUNIRAH BINTI HARON (E20131007556) 4.NORSYAWANI BINTI SUPIAN (E20131007577) LECTURER : CIK FATIMAH EXPERIMENT NO. : EXPERIMENT NO.1 TITLE : EXTRACTION OF DNA
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chemicals before use. Abide by specific warnings and directions. 3. Collect all materials needed for a procedure before proceeding. 4. Perform reactions under the hood when directed. Chemicals may be weighed and prepared at balance or lab tables‚ but tests should be carried out under the hood. 5.Acids and caustic chemicals are stored in the hood. Please do not take these chemicals from the hood. Procedure: PART 1: Metathetical reactions Precipitation reactions A1. Add a
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with the lab equipment such as the microscope‚ square glass‚ cover slips‚ etc.; to facilitate students to observe the pond water; and enable them to discern and identify the microbes as bacteria‚ algae‚ fungi‚ or protozoa within the pond water. Additionally‚ this lab experiment will permit students to observe and distinguish the yeast suspension from the other microbes. Data/Results: (Attached) Conclusion: The student acquired the fundamental skills in which to properly handle lab equipment
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concentrations of solutes in and out of the cell. Filtration is what is used to remove solid particles and they can be removed by passing through a liquid and gas. The information above is very important because it is exactly what everything in this lab will be about. It explains in detail what every single little definition and important
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