DENATURATION OF PROTEINS Abstract The experiment was done to be able to understand how various denaturants such as HCl and NaOH affects proteins. It was observed that different denaturants act upon or denature protein differently. This was determined using the principle of viscometry. An Ostwald viscometer was used to measure the viscosity of the prepared native‚ blank‚ denatured native and blank with denaturant solutions. The time required for the said solutions to pass through the viscometer
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Objective: 1) To test the presence of reducing sugars which is glucose in the Benedict’s test. 2) To test the presence of non-reducing sugars which is sucrose by using Hydrolysis then Benedict’s test. 3) To test the presence of the starch by using iodine test. 4) To test the presence of lipids in corn oils when using the Sudan Ш and Emulsion tests. 5) To test the presence of proteins in an egg albumen in the Biuret’s test. 6) To test the presence of Vitamin C and ascorbic acid in the DCPIP
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carbohydrates‚ proteins‚ lipids‚ nucleic acids. In this exercise we shall concentrate on carbohydrates‚ lipids‚ proteins‚ and we shall estimate the concentration of Vitamin C‚ an organic compound‚ in a solution‚ by the iodometric technique. • Carbohydrates include simple sugars‚ disaccharides and polysaccharides. They are the most important source of energy for most organisms. Polysaccharides change color in the presence of iodine solution: Glycogen gives a red-brown color and starch a dark blue–violet
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Abstract: This report presents the reason why the ocean does not freeze in the winter. Since the ocean consists of salt water‚ an experiment was done to test the effect of freezing on salt water compared to pure water. The experiment was conducted three times in order to obtain accurate results and eliminate errors. In addition to the salt and pure water experiment‚ a variation of this experiment was created to test other options. In the variation experiment the effect of freezing on sugar water was conducted
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Lab Report Abstract: The purpose of this lab was to see how magnesium reacts with oxygen. This reaction must be forced with heat. As magnesium changes to magnesium oxide the mass increased. Procedure: 1. Set up the stand and bunsen burne. 2. Go to crucible heat oven to obtain a crucible. 3. Weigh the crucible and lids mass. 4. Break up the magnesium ribbon on the crucible and weigh the mass of the magnesium‚ crucible‚ and lid. 5. Place the crucible on the ring stand over the flame. Allow
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Polypeptides‚ better known as proteins‚ carry out nearly all of a cell’s functions. A protein is made up of at least one or more amino acid polymers. The necessity of proteins for life is unequivocal‚ not only do they help to form the structures of cells but they act as enzymes in reactions of the body1. Enzymes are biological catalysts that lower the amount of activation energy needed in carrying out biochemical reactions1. Enzymes are responsible for almost every reaction that occurs in a cell
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beaker‚ the obvious answer is at the beginning of the experiment where the water was pure in nature and hypotonic. While the lowest concentration of free water would be at the end of the experiment where the glucose mixed with the distilled water; reducing the amount of free water concentration. One can also see that all the water did not move through to the tubing as the there would have been no water left in the
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of carbohydrates begin in the mouth‚ where an enzyme‚ salivary amylase (α-amylase;ptyalin) starts to breaking the polysaccharides (starch) into short polysaccharides (dextrin). Dextrin is a partial degradation of starch‚ shorter chains of maltose units. Salivary amylase is inactivated by stomach acid in the stomach and to a small extent‚ it continues breaking down starch (but there’s no enzymatic activities on carbohydrates in the stomach). An intestinal enzyme‚ pancreatic amylase‚ continues the activity
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Lab Report Janice Chisholm SCI207: Dependence of Man on the Environment Instructor: Lee Ott April 28‚ 2013 1. What patterns do you observe on the information table 4? While observing the information in table 4‚ it appears that the number of fish changes from time to time and the oxygen increases and / or decreases when this occurs. 2. Develop a hypothesis relating to the amount of dissolved oxygen measured in the water sample and the number of fish observed in the body of water?
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Potato Osmosis Biology SL - ATh INVESTIGATION “Potato Osmosis” INTRODUCTION Osmosis is a process that occurs at a cellular level that entails the spontaneous net movement of water through a semi-permeable membrane from a region of low solute concentration to an area of high solute concentration in order to equalize the level of water in each region. Involved in this process are hypotonic‚ hypertonic and isotonic solutions. A hypotonic solution is one with a lower osmotic pressure‚ indicating
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