In this lab we employed various assays utilizing a biuret reagent‚ coomassie brilliant blue reagent‚ and ultraviolet light in order to determine the identity of six unknown solutions and the concentration of a bovine serum albumin sample. We were given three samples that lacked protein‚ and three samples containing proteins‚ and using a spectrophotometer we assessed the amount of light absorbed versus the light transmitted‚ based on the principles of the Beer-Lambert Law. The three proteins used included
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that diatomic molecules make up majority of the Earth’s atmosphere? The prefix “di” means ‘’two’’ in Greek‚ so these diatomic molecules are made up of two atoms. In this lesson‚ we will discuss diatomic molecules and go over some examples. !!!What is a Diatomic Molecule? Diatomic molecules are all around us. The Earth’s atmosphere is mainly composed of about 78% oxygen and 21% nitrogen. The oxygen and nitrogen present in the atmosphere are both in the form of ‘’diatomic molecules’’‚ as shown below:
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T2:BIOCHEMISTRY AND ANALYSIS:DETECTION OF FATS‚PROTEINS AND CARBOHYDRATES OBJECTIVE The main purpose of the experiment is to understand some general tests that detect fats‚proteins and carbohydrates in foods. INTRODUCTION Carbohydrates are also known as sacharides. There are 4 main groups of carbohydrates‚which are monosaccharides‚ disaccharides‚ oligosaccharides and polysaccharides. Carbohydrates play an important role in living organism as it is the energy storage‚ and it also plays
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When fat is stored in the body‚ while were losing weight‚ the fat gets burned off. First‚ in activity 1.1‚ the lab data about jogging shows there was an -6 difference in calories before then after exercising. Also‚ on page 11‚ it states‚ “You know that when you go from sitting to running‚ you increase the amount of energy your body needs. This increase in amount of activity causes the changes you observed when you exercised in class.” This evidence means to me that‚ when we work out‚ we burn the
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Title : FOOD TESTS Aim : To determine whether given unknown solutions A‚B‚C‚D‚E‚F and G contain Reducing/NonReducing Sugars‚ Lipids‚ Proteins or Starch. Apparatus/Materials: - Bunsen burner - Solutions A‚ B‚ C‚ D‚ E‚ F and G (unknown) - Measuring Syringes - Stirring Rod - Beakers - Test tubes (7)plus holder and tray - Copper sulphate solution (CuSO4) - White dropping ray - tripod stand and mesh -stopwatch - Biuret͛s solution - Sodium Hydroxide solution (NaOH) (or Potassium Hydroxide
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To purify the protein in the cell lysate from lab 1 through nickel affinity chromatography. Protein purification should result in only one type of protein ideally‚ which is the protein of interest‚ wt-DHFR and mut-DHFR in this case. A pure protein allows for further analysis on the protein to be conducted‚ such as its concentration (Bradford assay)‚ its molecular weight‚ and its biological activity. 2. Overview of experiments Buffer Preparation Add the liquid sodium phosphate‚ solid sodium chloride
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What amount of amylase is the best to produce a better reaction with starch? A 1% solution of amylase‚ made from 0.15g of amylase added to 15ml of water‚ a 2% solution of amylase made of 0.3g of amylase added to 15ml of water‚ and a 0.5% solution of amylase made of 0.75g of amylase to 15ml of water were created. A 0.25% starch solution was also created‚ and 3ml was added to each of the 4 test tubes. 1 drop of iodine was added to each test tube. 4ml of the 1% solution was added to the first test
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compounds are soluble in water. This is due to the fact that water molecules are polar. Meaning that they have dipole bonds‚ which have a slightly positive and slightly negative end due to electronegativity differences between atoms. When ionic bonds are placed in water‚ they dissociate into their ions (positive and negative) due to the process of dissociation. These ions are then attracted to different dipoles present in the water molecule‚ in other words the negative ions are attracted to the positive
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reactions there are enzymes‚ biological catalysts‚ which help speed up metabolic reactions by lowering the energy barriers without being used up or altered in the reaction. (Campball‚ 2008) Every enzyme has an optimum pH at which it is most active. An increase or decrease in the pH of the solution will cause the enzyme to have a change in its three dimensional shape. If an enzyme is placed in an environment that is to basic or acidic the reaction will take longer to digest the starch because the enzyme shape
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Biological Macromolecules Lab Cells‚ the fundamental units of life‚ are composed of various combinations of organic macromolecules: carbohydrates‚ lipids‚ proteins‚ and nucleic acids. This lab exercise is designed to show you the qualitative tests commonly used to detect their presence. Objectives Test for the presence of monosaccharides by using the Benedict’s test Test for the presence of starch‚ a polysaccharide‚ by using the Lugol’s iodine test Test for the presence of lipids by using the
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