If pH > pI‚ then the protein will have a negative charge and if pH < pI‚ the protein will have a positive charge. Buffer I has a pH >5‚ meaning both proteins carry a negative charge and bind to the DEAE (a positively charged resin). (b) pH = pKa + log10(Base/Acid) [Base = mM of sodium acetate; Acid = mM of acetic acid] = 4.7 + log10 (40/40) = 4.7 In order for the catalase to elute from the column‚ it must have lost its negative charge and stopped binding to the DEAE. Lowering the pH
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Task 1 • Describe the structure of an enzyme as a protein‚ in terms of tertiary/ quaternary structures. 1) Primary Structure This is in reference to the order of way that amino acids are connected to form a protein. These are built up from 20 amino acids‚ and follow these structures o A carbon (the alpha carbon) bonded to the four groups below: o A hydrogen atom (H) o A Carboxyl group (-COOH) o An Amino group (-NH2) o A "variable" group or "R" group 2) Secondary Structure This is in reference
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Identifying Biological Molecules with Colorimetric Chemical Tests Objectives Perform a series of accurate tests on biological molecules to detect the presence of carbohydrates and proteins‚ as well as the action of an enzyme on specific molecules. Introduction Unknown biological molecules can be correctly identified using various tests‚ including colorimetric chemical tests. Colorimetric tests are when a chemical reagent‚ such as Benedict’s‚ is used on a substance or solution and a certain
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A protein is a Biological polymer made up of as different amino acids joined by peptide bonds. A protein is formed by amino acids which are the basic building block of a protein components of an amino acid include a :C-carbon atom‚ H-Hydrogen atom‚ H3N+ - Amino Group‚ COOH- - Carboxyl Group‚ R- this group varies from amino acid to amino acid. There are 20 amino acids‚ each differing in its R group. Two examples are: Glycine‚ its R group being H(Hydrogen) and Phenylalanine its R group being CH2 (Carbon
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Module 2 Section 2 EXPERIMENT: DNA & Protein Synthesis Exercise 1 – Modeling DNA 1. List the four bases which are found in DNA. (1 pt) The four bases found in DNA are cytosine‚ adenine‚ guanine and thymine. 2. Fit any six nucleotides together to form a row‚ then list the six nucleotides in the order you used them. Work with your model pieces and try fitting the bases together to make a double strand as shown in Figure 9 of the lab manual. Which nucleotides form pairs
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our lab we investigated alcoholic fermentation by using yeast‚ which can flourish in an low energy environment in anaerobic conditions. In this lab our goal was to discover the rate at which yeast will ferment different sized molecules of carbohydrates. In order to perform our experiment we made use of water‚ glucose‚ sucrose‚ and starch. It was hypothesized that glucose‚ sucrose‚ then starch would all be used to produce energy during fermentation. Being that glucose is a simple sugar‚ or monosaccharide
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Lab Report 2 Biological Media Meva Tinsley Georgia State University Lab Report 2 Introduction: Biological Media Microorganisms require nutrients and certain environmental conditions in order to thrive. In the lab‚ we use a Biological Media to aid in growth reproduction. Also referred to as a culture medium‚ a Biological Media is a substance used to support the growth of microorganisms. The two types of media most commonly used in Microbiology are selective media and differential
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DATE: 11/10/12 COURSE CODE: BIOL 2365 Comparative Biochemistry TITLE: Proteins and Amino Acids RESULTS: Table 1: The results of experiment 1; the Lowry Test Volume of Standard Protein/ Unknown (mL) Absorbance at 750 nm 0 0.000 0.1 0.017 0.3 0.135 0.3 0.155 0.5 0.230 0.7 0.323 0.7 0.310 1.0 0.457 1.0 Unknown 1a 0.463 1.0 Unknown 1b 0.433 1.0 Unknown 2a 0.237 1.0 Unknown 2b 0.159 Table 2: The results of Experiment 2; Ninhydrin Test Amino acid Color X
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The primary objective of this experiment is to determine the stress that various alcohols have on biological membranes. Membranes within cells are composed mainly of lipids and proteins and often serve to help maintain order within a cell by containing cellular materials. Different membranes have a variety of specific functions. One type of membrane-bound vacuole found in plant cells‚ the tonoplast‚ is quite large and usually contains water. In beet plants‚ this membrane-bound vacuole also contains
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see looking at the Total Protein column on Table 3‚ the most effective step with regard to the percent of remaining protein removed was affinity chromatography because it was able to remove 98.6% of the remaining proteins. In comparison to 81.93% removed during the 65% ammonium sulfate precipitation and 81.3% during the size exclusion. This means that the affinity chromatography removed a big percentage of contaminating proteins. However‚ removing this huge amount of protein left us with a small amount
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