This is a documentary released by BBC titled “Sugar VS Fat”‚ starred by Alexander Van Tulleken and Chris Van Tulleken. It is obvious that this is a program about the disadvantage and benefit of sugar as compared to fat. Yet‚ one should not judge the book by its cover‚ for the highlight of this documentary is the deadly combination of processed food with a ratio of 1:1 fat and sugar in it. Obesity is an anciently known problem. Over hundreds of years‚ scientists have estimated some possible cause
Premium
body fat percentage. It is typically the most accurate means of determining body fact percentage‚ and is based on measuring an individual’s body density. Archimedes’ principle explains that the upward buoyant force exerted on a body immersed in a fluid is equal to the weight of the fluid that the body displaces. Due to the fact that body fat is less dense than water‚ it increases one’s buoyancy‚ while the fat-free mass is denser than water‚ and which makes one sink. This allows percent fat to be
Premium Force Density Archimedes
In order to purify CelB2 protein bound to maltose-binding-protein‚ amylose affinity chromatography was performed. The amylose resin present in 20% ethanol was first diluted by adding 10 mL of 20 mM TrisHCl‚ pH 7.4‚ 0.2 M NaCl‚ 1 mM EDTA and centrifuged at 700 rpm for 5 minutes. After decanting the buffer‚ another 10 mL of TrisHCl‚ pH 7.4‚ 0.2 M NaCl‚ 1 mM EDTA was added to this resin solution and centrifuged at 700 rpm for another 5 minutes to further dilute the ethanol concentration in the resin
Premium Ethanol Enzyme Chromatography
BIOLOGY LABORATORY Biologically Important Molecules Carbohydrates‚ Proteins‚ Lipids‚ and Nucleic Acids Objectives In this lab you will learn to: 1. Perform tests to detect the presence of carbohydrates‚ lipids‚ proteins‚ and nucleic acids 2. Recognize the importance of a control in a biochemical test 3. Use biochemical tests to identify an unknown compound Introduction Organic molecules are those primarily made up of carbon‚ hydrogen and oxygen. The common organic compounds of living
Premium Amino acid Glucose Protein
Specificity of Protein-Ligand Binding 1). One can conclude that Orange G. has the lowest affinity for the albumin. In the experiment the concentration of Orange G that binds to protein was a lot less than either Ponceau S. or Bromophenol blue. The ligand with the highest affinity to BSA was a little more difficult to decipher. The experiment shows that all of the 2 µL of both Ponceau S. and Bromophenol blue bind to the BSA. However‚ when 5 µL of the ligand is added the BSA is saturated and cannot
Premium Protein Amino acid DNA
Molecular shapes play a part in how close two molecules can come to each other as when water molecules provide a solvent for other organic molecules. Cells are dynamic structures with constant activity The carbohydrates are unique in forming long chain polymer and also branching these chains. This is more complicated than protein where only 20 amino acids can form a long single chain. Here there can be numerous kinds of bonding forming a straight chain or long chain polymer. More over the number
Premium Oxygen Carbohydrate Polysaccharide
In the lab‚ three experiments were completed: the Iodine experiment‚ Benedict’s experiment and the Biuret experiment to test for starch and glycogen‚ reducing sugars and protein in the respective order. 12 solutions were given where 11 were known and the 12th was an unknown. The solutions that were supplied can be found in the results and observations section of this report. When completing such experiments‚ there are always controls used to make sure the procedure is followed and that no cross
Premium Chemistry Starch Glucose
Determining the sugar concentration of food samples is very important especially in industries where quality control is monitored. One method to determine the sugar concentration of reducing sugars is by heating with 3‚5 dinitrosalicylic acid(DNS) which produce a red-brown product Miller(1959)The reaction is direct‚thus the method is preferred over the Benedict’s test method. The concentration of the coloured complex can be determined with the spectrophotometer at Absorbance 540.The sugar concentration
Premium Water Boiling Sugar
brown sugar on the amount of CO2. To do this‚ white sugar was replaced with brown sugar and the amount of CO2 produced was calculated with a vernier gas pressure sensor. The control group was yeast and white sugar and the experimental group was yeast and brown sugar. The hypothesis that if brown sugar and yeast are mixed‚ then it will produce more CO2 than a mixture of white granulated sugar and yeast was accepted. The white sugar and yeast mixture had a slope of .003254 kPa/s. The brown sugar and
Premium Glucose Sugar Nutrition
Separation of Proteins and Mass Analysis Using SDS PAGE Biology 00-01L Abstract This experiment consisted of separating proteins into polypeptides using a method called SDS PAGE which is a type of electrophoresis. The polypeptides had different masses‚ so each polypeptide traveled a different distance and this was an essential part of the lab which demonstrated that there exists a relationship between the distance traveled by the protein and the mass of the protein. This relationship was graphed
Premium Protein Molecular biology Gel electrophoresis